Yetong Qi, Jiahui Wu, Zhu Yang, Hongjun Li, Lang Liu, Haixia Wang, Xinyuan Sun, Xinya Wu, Jiahui Nie, Jing Zhou, Meng Xu, Xintong Wu, Susan Breen, Ruimin Yu, Dong Cheng, Qingguo Sun, Huishan Qiu, Yingtao Zuo, Petra C. Boevink, Paul R. J. Birch, Zhendong Tian
{"title":"叶绿体伸长因子同时促进产量和防御,打破了生长与免疫之间的平衡","authors":"Yetong Qi, Jiahui Wu, Zhu Yang, Hongjun Li, Lang Liu, Haixia Wang, Xinyuan Sun, Xinya Wu, Jiahui Nie, Jing Zhou, Meng Xu, Xintong Wu, Susan Breen, Ruimin Yu, Dong Cheng, Qingguo Sun, Huishan Qiu, Yingtao Zuo, Petra C. Boevink, Paul R. J. Birch, Zhendong Tian","doi":"10.1038/s41477-024-01793-x","DOIUrl":null,"url":null,"abstract":"Chloroplasts regulate plant development and immunity. Here we report that potato chloroplast elongation factors StTuA and StTuB, targeted by Phytophthora infestans RXLR effector Pi22926, positively regulate immunity and growth. Plants expressing Pi22926, or silenced for TuA/B, show increased P. infestans susceptibility and decreased photosynthesis, plant growth and tuber yield. By contrast, StTuA/B overexpression reduces susceptibility, elevates chloroplast-derived reactive oxygen species production and increases photosynthesis and potato tuber yield by enhancing chloroplast protein translation. Another plant target of Pi22926, StMAP3Kβ2, interacts with StTuB, phosphorylating it to promote its translocation into chloroplasts. However, Pi22926 attenuates StTuB association with StMAP3Kβ2 and phosphorylation. This reduces StTuB translocation into chloroplasts, leading to its proteasome-mediated turnover in the cytoplasm. We uncover new mechanisms by which a pathogen effector inhibits immunity by disrupting key chloroplast functions. This work shows that StTuA/B break the growth–immunity trade-off, promoting both disease resistance and yield, revealing the enormous potential of chloroplast biology in crop breeding. Enhanced expression of chloroplast elongation factors StTuA/B increases potato disease resistance and photosynthesis, while oomycete effector Pi22926 inhibits their entry into the chloroplasts by disrupting their phosphorylation by StMAP3Kβ2.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8000,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chloroplast elongation factors break the growth–immunity trade-off by simultaneously promoting yield and defence\",\"authors\":\"Yetong Qi, Jiahui Wu, Zhu Yang, Hongjun Li, Lang Liu, Haixia Wang, Xinyuan Sun, Xinya Wu, Jiahui Nie, Jing Zhou, Meng Xu, Xintong Wu, Susan Breen, Ruimin Yu, Dong Cheng, Qingguo Sun, Huishan Qiu, Yingtao Zuo, Petra C. Boevink, Paul R. J. Birch, Zhendong Tian\",\"doi\":\"10.1038/s41477-024-01793-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Chloroplasts regulate plant development and immunity. Here we report that potato chloroplast elongation factors StTuA and StTuB, targeted by Phytophthora infestans RXLR effector Pi22926, positively regulate immunity and growth. Plants expressing Pi22926, or silenced for TuA/B, show increased P. infestans susceptibility and decreased photosynthesis, plant growth and tuber yield. By contrast, StTuA/B overexpression reduces susceptibility, elevates chloroplast-derived reactive oxygen species production and increases photosynthesis and potato tuber yield by enhancing chloroplast protein translation. Another plant target of Pi22926, StMAP3Kβ2, interacts with StTuB, phosphorylating it to promote its translocation into chloroplasts. However, Pi22926 attenuates StTuB association with StMAP3Kβ2 and phosphorylation. This reduces StTuB translocation into chloroplasts, leading to its proteasome-mediated turnover in the cytoplasm. We uncover new mechanisms by which a pathogen effector inhibits immunity by disrupting key chloroplast functions. This work shows that StTuA/B break the growth–immunity trade-off, promoting both disease resistance and yield, revealing the enormous potential of chloroplast biology in crop breeding. Enhanced expression of chloroplast elongation factors StTuA/B increases potato disease resistance and photosynthesis, while oomycete effector Pi22926 inhibits their entry into the chloroplasts by disrupting their phosphorylation by StMAP3Kβ2.\",\"PeriodicalId\":18904,\"journal\":{\"name\":\"Nature Plants\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":15.8000,\"publicationDate\":\"2024-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nature Plants\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.nature.com/articles/s41477-024-01793-x\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Plants","FirstCategoryId":"99","ListUrlMain":"https://www.nature.com/articles/s41477-024-01793-x","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Chloroplast elongation factors break the growth–immunity trade-off by simultaneously promoting yield and defence
Chloroplasts regulate plant development and immunity. Here we report that potato chloroplast elongation factors StTuA and StTuB, targeted by Phytophthora infestans RXLR effector Pi22926, positively regulate immunity and growth. Plants expressing Pi22926, or silenced for TuA/B, show increased P. infestans susceptibility and decreased photosynthesis, plant growth and tuber yield. By contrast, StTuA/B overexpression reduces susceptibility, elevates chloroplast-derived reactive oxygen species production and increases photosynthesis and potato tuber yield by enhancing chloroplast protein translation. Another plant target of Pi22926, StMAP3Kβ2, interacts with StTuB, phosphorylating it to promote its translocation into chloroplasts. However, Pi22926 attenuates StTuB association with StMAP3Kβ2 and phosphorylation. This reduces StTuB translocation into chloroplasts, leading to its proteasome-mediated turnover in the cytoplasm. We uncover new mechanisms by which a pathogen effector inhibits immunity by disrupting key chloroplast functions. This work shows that StTuA/B break the growth–immunity trade-off, promoting both disease resistance and yield, revealing the enormous potential of chloroplast biology in crop breeding. Enhanced expression of chloroplast elongation factors StTuA/B increases potato disease resistance and photosynthesis, while oomycete effector Pi22926 inhibits their entry into the chloroplasts by disrupting their phosphorylation by StMAP3Kβ2.
期刊介绍:
Nature Plants is an online-only, monthly journal publishing the best research on plants — from their evolution, development, metabolism and environmental interactions to their societal significance.