核因子 Y-POLYCOMB REPRESSIVE COMPLEX2 动态协调小麦淀粉和种子贮藏蛋白的生物合成

Jinchao Chen, Long Zhao, Haoran Li, Changfeng Yang, Xuelei Lin, Yujing Lin, Hao Zhang, Mengxia Zhang, Xiaomin Bie, Peng Zhao, Shengbao Xu, David Seung, Xiansheng Zhang, Xueyong Zhang, Yingyin Yao, Dongzhi Wang, Jun Xiao
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引用次数: 0

摘要

谷物的胚乳控制着淀粉和种子贮藏蛋白(SSP)的产生,因此在决定谷物产量和种子质量方面起着重要作用。在这项研究中,我们在小麦(Triticum aestivum L.)中发现了一种特异的核因子-Y(NF-Y)三聚体复合物,由 TaNF-YA3-D、TaNF-YB7-B 和 TaNF-YC6-B 组成,在谷粒充实过程中在胚乳中表现出强劲的表达。敲除 TaNF-YA3 或 TaNF-YC6 会导致淀粉含量降低,但谷蛋白含量增加。TaNF-Y 通过抑制细胞膜小 ADP-葡萄糖焦磷酸化酶 1a(TacAGPS1a)的抑制因子 TaNAC019、蔗糖合成酶 2(TaSuS2)以及其他参与淀粉生物合成的基因,间接促进了淀粉生物合成基因的表达。相反,TaNF-Y 直接抑制麸质-γ-700(TaGli-γ-700)和低分子量-400(TaLMW-400)的表达。此外,TaNF-Y成分与多聚核糖抑制复合体2(PRC2)的组蛋白甲基转移酶亚基SWINGER(TaSWN)相互作用,通过组蛋白H3在赖氨酸27处的三甲基化(H3K27me3)修饰抑制TaNAC019、TaGli-γ-700和TaLMW-400的表达。值得注意的是,核心 PRC2 亚基 FERTILIZATION INDEPENDENT ENDOSPERM(TaFIE)的弱突变降低了淀粉含量,但提高了麦胶蛋白和 LMW-GS 的含量。有趣的是,TaNF-YB7-B 编码区内的序列变异与淀粉和 SSP 含量的差异有关。不同的 TaNF-YB7-B 单倍型会影响其与 TaSWN-B 的相互作用,从而影响对 TaNAC019 和 TaGli-γ-700 等靶标的抑制。我们的发现揭示了TaNF-Y-PRC2介导的小麦胚乳发育表观遗传调控的复杂分子机制。操纵 TaNF-Y 复合物有望优化谷物产量和提高谷物品质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
NUCLEAR FACTOR-Y–POLYCOMB REPRESSIVE COMPLEX2 dynamically orchestrates starch and seed storage protein biosynthesis in wheat
The endosperm in cereal grains is instrumental in determining grain yield and seed quality, as it controls starch and seed storage protein (SSP) production. In this study, we identified a specific nuclear factor-Y (NF-Y) trimeric complex in wheat (Triticum aestivum L.), consisting of TaNF-YA3-D, TaNF-YB7-B, and TaNF-YC6-B, and exhibiting robust expression within the endosperm during grain filling. Knockdown of either TaNF-YA3 or TaNF-YC6 led to reduced starch but increased gluten protein levels. TaNF-Y indirectly boosted starch biosynthesis genes by repressing TaNAC019, a repressor of cytosolic small ADP-glucose pyrophosphorylase 1a (TacAGPS1a), sucrose synthase 2 (TaSuS2), and other genes involved in starch biosynthesis. Conversely, TaNF-Y directly inhibited the expression of Gliadin-γ-700 (TaGli-γ-700) and low molecular weight-400 (TaLMW-400). Furthermore, TaNF-Y components interacted with SWINGER (TaSWN), the histone methyltransferase subunit of Polycomb repressive complex 2 (PRC2), to repress TaNAC019, TaGli-γ-700, and TaLMW-400 expression through trimethylation of histone H3 at lysine 27 (H3K27me3) modification. Notably, weak mutation of FERTILIZATION INDEPENDENT ENDOSPERM (TaFIE), a core PRC2 subunit, reduced starch but elevated gliadin and LMW-GS contents. Intriguingly, sequence variation within the TaNF-YB7-B coding region was linked to differences in starch and SSP content. Distinct TaNF-YB7-B haplotypes affect its interaction with TaSWN-B, influencing the repression of targets like TaNAC019 and TaGli-γ-700. Our findings illuminate the intricate molecular mechanisms governing TaNF-Y–PRC2-mediated epigenetic regulation for wheat endosperm development. Manipulating the TaNF-Y complex holds potential for optimizing grain yield and enhancing grain quality.
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