Qiuping Yao , Longlin Pu , Boyu Dong , Dequan Zhu , Wenwen Wu , Qiong Yang
{"title":"超声降解对皂荚多糖理化性质和抗氧化性的影响","authors":"Qiuping Yao , Longlin Pu , Boyu Dong , Dequan Zhu , Wenwen Wu , Qiong Yang","doi":"10.1016/j.carres.2024.109272","DOIUrl":null,"url":null,"abstract":"<div><p>In this study, two degraded polysaccharides from <em>Gleditsia sinensis</em> seed were obtained under ultrasonic power treatments of 300 and 450 W. The physicochemical properties, structural characteristics, and antioxidant activities of the degraded and undegraded polysaccharides were studied and compared. Ion exchange chromatography and methylation analysis showed that the polysaccharides had similar basic structural features and were composed of the same monosaccharide units before and after degradation, but the ultrasonic treatment increased the total monosaccharide content and changed the Mannose/Galactose value. Furthermore, with the increase in the ultrasonic power, the molecular weight and intrinsic viscosity of polysaccharides decreased, and the micromorphology became looser. The scavenging capacities for 1,1-diphenyl-2-picrylhydrazyl and hydroxyl free radicals and the reducing ability were significantly increased by the ultrasonic treatment. In conclusion, ultrasonic treatment may be an effective way to improve the antioxidant activities of polysaccharides from <em>G</em>. <em>sinensis</em> seed, and further studies on its antioxidant mechanism are still needed.</p></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"545 ","pages":"Article 109272"},"PeriodicalIF":2.4000,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of ultrasonic degradation on physicochemical and antioxidant properties of Gleditsia sinensis seed polysaccharides\",\"authors\":\"Qiuping Yao , Longlin Pu , Boyu Dong , Dequan Zhu , Wenwen Wu , Qiong Yang\",\"doi\":\"10.1016/j.carres.2024.109272\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In this study, two degraded polysaccharides from <em>Gleditsia sinensis</em> seed were obtained under ultrasonic power treatments of 300 and 450 W. The physicochemical properties, structural characteristics, and antioxidant activities of the degraded and undegraded polysaccharides were studied and compared. Ion exchange chromatography and methylation analysis showed that the polysaccharides had similar basic structural features and were composed of the same monosaccharide units before and after degradation, but the ultrasonic treatment increased the total monosaccharide content and changed the Mannose/Galactose value. Furthermore, with the increase in the ultrasonic power, the molecular weight and intrinsic viscosity of polysaccharides decreased, and the micromorphology became looser. The scavenging capacities for 1,1-diphenyl-2-picrylhydrazyl and hydroxyl free radicals and the reducing ability were significantly increased by the ultrasonic treatment. In conclusion, ultrasonic treatment may be an effective way to improve the antioxidant activities of polysaccharides from <em>G</em>. <em>sinensis</em> seed, and further studies on its antioxidant mechanism are still needed.</p></div>\",\"PeriodicalId\":9415,\"journal\":{\"name\":\"Carbohydrate Research\",\"volume\":\"545 \",\"pages\":\"Article 109272\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2024-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Carbohydrate Research\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0008621524002519\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbohydrate Research","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0008621524002519","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Effects of ultrasonic degradation on physicochemical and antioxidant properties of Gleditsia sinensis seed polysaccharides
In this study, two degraded polysaccharides from Gleditsia sinensis seed were obtained under ultrasonic power treatments of 300 and 450 W. The physicochemical properties, structural characteristics, and antioxidant activities of the degraded and undegraded polysaccharides were studied and compared. Ion exchange chromatography and methylation analysis showed that the polysaccharides had similar basic structural features and were composed of the same monosaccharide units before and after degradation, but the ultrasonic treatment increased the total monosaccharide content and changed the Mannose/Galactose value. Furthermore, with the increase in the ultrasonic power, the molecular weight and intrinsic viscosity of polysaccharides decreased, and the micromorphology became looser. The scavenging capacities for 1,1-diphenyl-2-picrylhydrazyl and hydroxyl free radicals and the reducing ability were significantly increased by the ultrasonic treatment. In conclusion, ultrasonic treatment may be an effective way to improve the antioxidant activities of polysaccharides from G. sinensis seed, and further studies on its antioxidant mechanism are still needed.
期刊介绍：
Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects.
Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence.
Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".
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