Deeikshana T , Poorniammal R , Shandeep S G , Vijay S , Prabhu S
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To decipher the mode of action of compounds, molecular docking studies were performed with potential protein targets such as acetylcholinesterase, β-1,4-endoglucanase, glutathione S-transferase-1, cytochrome <em>c</em> oxidase, G-protein coupled receptor and Fatty acid and retinol-binding proteins of <em>M. incognita</em>. The results revealed that among eight compounds from the L19 fraction, malonate and pidopidon exhibited greater binding affinity towards the selected protein targets of <em>M. incognita</em>. <em>In vitro</em> studies with malonate and pidopidon against <em>M. incognita</em> showcased a 99% reduction in egg hatching and juvenile mortality. Moreover, greenhouse experiments revealed that malonate compounds not only reduced 94% of the <em>M. incognita</em> population but also enhanced the plant growth parameters in tomato by 60%. 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引用次数: 0
摘要
研究人员尝试评估纯化的 Xenorhabdus nematophila 生物活性化合物对 Meloidogyne incognita 的防治效果。为了提取纯化的化合物,使用了固体支撑液-液萃取系统,流速为 1 mL/min,以纯化生物活性分子。对化合物进行单独收集、浓缩并对 M. incognita 进行评估。在 25 个馏分中,L19 馏分对卵孵化和幼虫死亡率的抑制率为 98%。这些生物大分子通过液相色谱-质谱(LC-MS)技术进行了鉴定。为了破译化合物的作用模式,研究人员与潜在的蛋白靶标(如乙酰胆碱酯酶、β-1,4-内切葡聚糖酶、谷胱甘肽 S-转移酶-1、细胞色素 c 氧化酶、G 蛋白偶联受体以及麦蛾的脂肪酸和视黄醇结合蛋白)进行了分子对接研究。研究结果表明,在 L19 馏分的八种化合物中,丙二酸盐和匹多匹东对 M. incognita 的选定蛋白靶标具有更强的结合亲和力。利用丙二酸盐和pidopidon对M. incognita进行的体外研究显示,卵孵化率和幼虫死亡率降低了99%。此外,温室实验表明,丙二酸盐化合物不仅减少了 94% 的 M. incognita 数量,还提高了番茄植物生长参数的 60%。因此,本研究在利用 X. nematophila 的杀线虫化合物方面具有新颖性,为探索 pidopidon 和丙二酸盐作为新型杀线虫化合物管理 M. incognita 提供了机会。
Exploring nematicidal biomolecules from Xenorhabdus nematophila as a novel source for Meloidogyne incognita management
Attempts were made to evaluate the purified bioactive compounds of Xenorhabdus nematophila against Meloidogyne incognita. In order to extract the purified compounds, a solid-supported liquid-liquid extraction system with a flow rate (1 mL/min) was used to purify bioactive molecules. Compounds were individually collected concentrated and evaluated against M. incognita. Among 25 fractions the L19 fraction, exhibited 98% inhibition in egg hatching and mortality of juveniles. The biomolecules were identified through Liquid Chromatography- Mass Spectroscopy (LC-MS) technique. To decipher the mode of action of compounds, molecular docking studies were performed with potential protein targets such as acetylcholinesterase, β-1,4-endoglucanase, glutathione S-transferase-1, cytochrome c oxidase, G-protein coupled receptor and Fatty acid and retinol-binding proteins of M. incognita. The results revealed that among eight compounds from the L19 fraction, malonate and pidopidon exhibited greater binding affinity towards the selected protein targets of M. incognita. In vitro studies with malonate and pidopidon against M. incognita showcased a 99% reduction in egg hatching and juvenile mortality. Moreover, greenhouse experiments revealed that malonate compounds not only reduced 94% of the M. incognita population but also enhanced the plant growth parameters in tomato by 60%. Hence the present study stands novel in exploiting the nematicidal compounds from X. nematophila giving limelight to explore pidopidon and malonate as novel nematicidal compounds for the management of M. incognita.
期刊介绍:
Toxicon has an open access mirror Toxicon: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. An introductory offer Toxicon: X - full waiver of the Open Access fee.
Toxicon''s "aims and scope" are to publish:
-articles containing the results of original research on problems related to toxins derived from animals, plants and microorganisms
-papers on novel findings related to the chemical, pharmacological, toxicological, and immunological properties of natural toxins
-molecular biological studies of toxins and other genes from poisonous and venomous organisms that advance understanding of the role or function of toxins
-clinical observations on poisoning and envenoming where a new therapeutic principle has been proposed or a decidedly superior clinical result has been obtained.
-material on the use of toxins as tools in studying biological processes and material on subjects related to venom and antivenom problems.
-articles on the translational application of toxins, for example as drugs and insecticides
-epidemiological studies on envenoming or poisoning, so long as they highlight a previously unrecognised medical problem or provide insight into the prevention or medical treatment of envenoming or poisoning. Retrospective surveys of hospital records, especially those lacking species identification, will not be considered for publication. Properly designed prospective community-based surveys are strongly encouraged.
-articles describing well-known activities of venoms, such as antibacterial, anticancer, and analgesic activities of arachnid venoms, without any attempt to define the mechanism of action or purify the active component, will not be considered for publication in Toxicon.
-review articles on problems related to toxinology.
To encourage the exchange of ideas, sections of the journal may be devoted to Short Communications, Letters to the Editor and activities of the affiliated societies.