椰糠上适应性固定微生物群落对对硝基苯酚的降解性能增强

IF 4.1 2区 环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Bao-Ngoc T. Le , Van-Anh T. Nguyen , Nguyen-Phuong Nguyen , Hong-Nhung Nguyen , Thanh-Truc H. Phan , Thuy-Huong Nguyen , Thuy-Phuong T. Pham , Hoang-Duy P. Nguyen
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引用次数: 0

摘要

众所周知,包括对硝基苯酚(p-NP)在内的硝基苯酚污染物因其持久性、毒性和在水源中的广泛分布而对环境造成有害影响。虽然与化学或物理方法相比,生物降解通常能更有效地去除有机污染物,但降解对硝基苯酚等持久性有毒化合物仍具有挑战性。本研究将来自食品加工废水的微生物群落固定在椰糠上,使其适应对-NP,然后用于对-NP 的生物降解。光谱分析证明了适应性微生物群落的有效生物降解性能,在 38 分钟内对 50 mg L-1 p-NP 的降解率达到 99%,在 4.65 小时内对 250 mg L-1 p-NP 的降解率达到 99%。固定化细胞的降解能力是在广泛的搅拌速度、温度、pH 值和 p-NP 溶液体积范围内测定的。经处理溶液的化学需氧量(COD)测量值和原位二氧化碳生成量证实了 p-NP 的完全矿化。值得注意的是,适应性固定化微生物群落的对-NP 降解性能在最初的 40 天内保持稳定,仅在 4 °C 低温保存 47 天后略有下降。在连续 54 次运行中,p-NP 平均降解率保持在 0.75 mg L-1 min-1 的水平。通过 16S 代谢编码分析确定了微生物多样性的显著变化。未适应的微生物群落由多种多样的菌属组成,而适应的群落则显示出多样性的降低,富集了一些已知能降解 p-NP 的特定菌属,如微球菌科的不明成员、Paenarthrobacter 菌属和 Zoogloea 菌属。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhanced degradation performance toward para-nitrophenol of adapted immobilized microbial community on coconut coir

Nitrophenol pollutants, including para-nitrophenol (p-NP), are known for their harmful environmental impact due to their persistence, toxicity, and widespread distribution in water sources. While biodegradation generally offers a more effective removal of organic pollutants compared to chemical or physical methods, degrading persistent and toxic compounds like p-NP remains challenging. In this study, a microbial community derived from food processing wastewater was immobilized on coconut coir and adapted to p-NP before being employed for p-NP biodegradation. The spectroscopic analysis demonstrates the effective biodegradation performance of the adapted microbial community, achieving 99% degradation of 50 mg L⁻1 p-NP in 38 min and 250 mg L⁻1 p-NP in 4.65 h. The degradation ability of immobilized cells was determined across a broad range of stirring speeds, temperatures, pH levels, and p-NP solution volumes. Complete mineralization of p-NP was confirmed by chemical oxygen demand (COD) measurements of the treated solution and in-situ CO2 generation. Notably, the p-NP degradation performance of the adapted immobilized microbial community remained stable for the first 40 days, with only a slight decrease observed after 47 days of cold preservation at 4 °C. An average p-NP degradation rate of 0.75 mg L⁻1 min⁻1 was maintained over 54 consecutive runs. Significant alterations in microbial diversity were identified through 16S metabarcoding analysis. The unadapted microbial community comprised a diverse range of genera, while the adapted community showed reduced diversity with an enrichment of specific genera known for p-NP degradation, such as unidentified members of the Micrococcaceae family, Paenarthrobacter spp., and Zoogloea spp.

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来源期刊
CiteScore
9.60
自引率
10.40%
发文量
107
审稿时长
21 days
期刊介绍: International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.
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