{"title":"PdbbHLH1 转录因子提高了杨树 Davidiana × P. bolleana 的耐旱性","authors":"","doi":"10.1016/j.indcrop.2024.119683","DOIUrl":null,"url":null,"abstract":"<div><p>The basic helix-loop-helix (bHLH) family of transcription factors (TFs) plays a critical role in regulating plant resistance to various abiotic stresses, including drought, low temperature, high salinity, and iron deficiency, as well as in overseeing key processes in plant growth and development. Despite the well-documented functions of bHLH TFs in many plant species, their roles in <em>Populus davidiana</em> × <em>P. bolleana</em> under abiotic stress conditions remain largely unexplored. This study identified a bHLH gene, designated as <em>PdbbHLH1</em>, whose expression is markedly upregulated in response to PEG<sub>6000</sub> treatment. To investigate its function, the research generated transgenic Populus plants overexpressing (OE) <em>PdbbHLH1</em> through <em>Agrobacterium tumefaciens</em>-mediated transformation. Stress experiments demonstrated that OE <em>PdbbHLH1</em> significantly enhanced the drought resistance of these transgenic plants, as evidenced by increased fresh weight and chlorophyll content compared to wild-type (WT) plants under drought conditions. Physiological analyses further revealed that <em>PdbbHLH1</em>-OE plants exhibited lower levels of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA), indicating reduced cellular damage and a more robust reactive oxygen species (ROS) scavenging capability than WT plants. Furthermore, the expression of drought resistance-related genes, such as <em>PdbAAO</em>, <em>PdbGST</em>, and <em>PdbPOD</em>, was significantly upregulated in OE plants post-drought stress. Notably, <em>PdbbHLH1</em> was shown to directly bind to the promoters of <em>PdbPOD1</em> and <em>PdbPOD4</em>, activating their expression and thereby enhancing the drought resistance of transgenic <em>Populus davidiana</em> × <em>P. bolleana</em>. Collectively, these results suggest that <em>PdbbHLH1</em> is a key regulator of drought tolerance in <em>Populus</em>, modulating this response through the enhancement of antioxidant enzyme activities at the physiological level<em>.</em></p></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":null,"pages":null},"PeriodicalIF":5.6000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"PdbbHLH1 transcription factor improved drought tolerance of Populus davidiana × P. bolleana\",\"authors\":\"\",\"doi\":\"10.1016/j.indcrop.2024.119683\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The basic helix-loop-helix (bHLH) family of transcription factors (TFs) plays a critical role in regulating plant resistance to various abiotic stresses, including drought, low temperature, high salinity, and iron deficiency, as well as in overseeing key processes in plant growth and development. Despite the well-documented functions of bHLH TFs in many plant species, their roles in <em>Populus davidiana</em> × <em>P. bolleana</em> under abiotic stress conditions remain largely unexplored. This study identified a bHLH gene, designated as <em>PdbbHLH1</em>, whose expression is markedly upregulated in response to PEG<sub>6000</sub> treatment. To investigate its function, the research generated transgenic Populus plants overexpressing (OE) <em>PdbbHLH1</em> through <em>Agrobacterium tumefaciens</em>-mediated transformation. Stress experiments demonstrated that OE <em>PdbbHLH1</em> significantly enhanced the drought resistance of these transgenic plants, as evidenced by increased fresh weight and chlorophyll content compared to wild-type (WT) plants under drought conditions. Physiological analyses further revealed that <em>PdbbHLH1</em>-OE plants exhibited lower levels of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA), indicating reduced cellular damage and a more robust reactive oxygen species (ROS) scavenging capability than WT plants. Furthermore, the expression of drought resistance-related genes, such as <em>PdbAAO</em>, <em>PdbGST</em>, and <em>PdbPOD</em>, was significantly upregulated in OE plants post-drought stress. Notably, <em>PdbbHLH1</em> was shown to directly bind to the promoters of <em>PdbPOD1</em> and <em>PdbPOD4</em>, activating their expression and thereby enhancing the drought resistance of transgenic <em>Populus davidiana</em> × <em>P. bolleana</em>. Collectively, these results suggest that <em>PdbbHLH1</em> is a key regulator of drought tolerance in <em>Populus</em>, modulating this response through the enhancement of antioxidant enzyme activities at the physiological level<em>.</em></p></div>\",\"PeriodicalId\":13581,\"journal\":{\"name\":\"Industrial Crops and Products\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.6000,\"publicationDate\":\"2024-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Industrial Crops and Products\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0926669024016601\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURAL ENGINEERING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Industrial Crops and Products","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0926669024016601","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}
PdbbHLH1 transcription factor improved drought tolerance of Populus davidiana × P. bolleana
The basic helix-loop-helix (bHLH) family of transcription factors (TFs) plays a critical role in regulating plant resistance to various abiotic stresses, including drought, low temperature, high salinity, and iron deficiency, as well as in overseeing key processes in plant growth and development. Despite the well-documented functions of bHLH TFs in many plant species, their roles in Populus davidiana × P. bolleana under abiotic stress conditions remain largely unexplored. This study identified a bHLH gene, designated as PdbbHLH1, whose expression is markedly upregulated in response to PEG6000 treatment. To investigate its function, the research generated transgenic Populus plants overexpressing (OE) PdbbHLH1 through Agrobacterium tumefaciens-mediated transformation. Stress experiments demonstrated that OE PdbbHLH1 significantly enhanced the drought resistance of these transgenic plants, as evidenced by increased fresh weight and chlorophyll content compared to wild-type (WT) plants under drought conditions. Physiological analyses further revealed that PdbbHLH1-OE plants exhibited lower levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA), indicating reduced cellular damage and a more robust reactive oxygen species (ROS) scavenging capability than WT plants. Furthermore, the expression of drought resistance-related genes, such as PdbAAO, PdbGST, and PdbPOD, was significantly upregulated in OE plants post-drought stress. Notably, PdbbHLH1 was shown to directly bind to the promoters of PdbPOD1 and PdbPOD4, activating their expression and thereby enhancing the drought resistance of transgenic Populus davidiana × P. bolleana. Collectively, these results suggest that PdbbHLH1 is a key regulator of drought tolerance in Populus, modulating this response through the enhancement of antioxidant enzyme activities at the physiological level.
期刊介绍:
Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.