Junmei Jiang, Nuo Zhang, Atul Kumar Srivastava, Guandi He, Zheng Tai, Zehui Wang, Sanwei Yang, Xin Xie, Xiangyang Li
{"title":"超氧化物歧化酶通过与超氧化物歧化酶的铜伴侣蛋白相互作用,积极调节高粱对铜/锌毒性的耐受性","authors":"Junmei Jiang, Nuo Zhang, Atul Kumar Srivastava, Guandi He, Zheng Tai, Zehui Wang, Sanwei Yang, Xin Xie, Xiangyang Li","doi":"10.1016/j.jhazmat.2024.135828","DOIUrl":null,"url":null,"abstract":"<p>Heavy metal stress threatens plant growth and productivity. In this study, we investigated the effects of CuSO<sub>4</sub> and ZnSO<sub>4</sub> toxicity on sorghum seedlings, focusing on their impact on biomass, germination rates, growth parameters, antioxidant enzyme activities, gene expression profiles, and stress resistance mechanisms. As a result, eight sorghum superoxide dismutase (<em>SOD</em>) genes were identified, and their evolutionary relationships with <em>cis</em>-acting regulatory elements and their expressional patterns were evaluated. Integrating transcriptomic data revealed a key SOD member SbCSD1 that might contribute to plant abiotic stress resistance. Furthermore, <em>SbCSD1</em> overexpression enhanced plant tolerance to CuSO<sub>4</sub> and ZnSO<sub>4</sub> stress by regulating SOD activity and interacting with copper chaperone for superoxide dismutase 1 (CCS1) in the plant nucleus and cytoplasm. Meanwhile, silencing <em>CCS1</em> in <em>SbCSD1</em>-overexpressing plants revealed that SbCSD1 and CCS1 synergistically contribute to Cu stress tolerance. By integrating transcriptomic and genetic data, herein we provide novel insights into the orchestration of plant responses to heavy-metal stress in sorghum by SOD.</p>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":null,"pages":null},"PeriodicalIF":12.2000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Superoxide dismutase positively regulates Cu/Zn toxicity tolerance in Sorghum bicolor by interacting with Cu chaperone for superoxide dismutase\",\"authors\":\"Junmei Jiang, Nuo Zhang, Atul Kumar Srivastava, Guandi He, Zheng Tai, Zehui Wang, Sanwei Yang, Xin Xie, Xiangyang Li\",\"doi\":\"10.1016/j.jhazmat.2024.135828\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Heavy metal stress threatens plant growth and productivity. In this study, we investigated the effects of CuSO<sub>4</sub> and ZnSO<sub>4</sub> toxicity on sorghum seedlings, focusing on their impact on biomass, germination rates, growth parameters, antioxidant enzyme activities, gene expression profiles, and stress resistance mechanisms. As a result, eight sorghum superoxide dismutase (<em>SOD</em>) genes were identified, and their evolutionary relationships with <em>cis</em>-acting regulatory elements and their expressional patterns were evaluated. Integrating transcriptomic data revealed a key SOD member SbCSD1 that might contribute to plant abiotic stress resistance. Furthermore, <em>SbCSD1</em> overexpression enhanced plant tolerance to CuSO<sub>4</sub> and ZnSO<sub>4</sub> stress by regulating SOD activity and interacting with copper chaperone for superoxide dismutase 1 (CCS1) in the plant nucleus and cytoplasm. Meanwhile, silencing <em>CCS1</em> in <em>SbCSD1</em>-overexpressing plants revealed that SbCSD1 and CCS1 synergistically contribute to Cu stress tolerance. By integrating transcriptomic and genetic data, herein we provide novel insights into the orchestration of plant responses to heavy-metal stress in sorghum by SOD.</p>\",\"PeriodicalId\":361,\"journal\":{\"name\":\"Journal of Hazardous Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":12.2000,\"publicationDate\":\"2024-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Hazardous Materials\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jhazmat.2024.135828\",\"RegionNum\":1,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Hazardous Materials","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.jhazmat.2024.135828","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Superoxide dismutase positively regulates Cu/Zn toxicity tolerance in Sorghum bicolor by interacting with Cu chaperone for superoxide dismutase
Heavy metal stress threatens plant growth and productivity. In this study, we investigated the effects of CuSO4 and ZnSO4 toxicity on sorghum seedlings, focusing on their impact on biomass, germination rates, growth parameters, antioxidant enzyme activities, gene expression profiles, and stress resistance mechanisms. As a result, eight sorghum superoxide dismutase (SOD) genes were identified, and their evolutionary relationships with cis-acting regulatory elements and their expressional patterns were evaluated. Integrating transcriptomic data revealed a key SOD member SbCSD1 that might contribute to plant abiotic stress resistance. Furthermore, SbCSD1 overexpression enhanced plant tolerance to CuSO4 and ZnSO4 stress by regulating SOD activity and interacting with copper chaperone for superoxide dismutase 1 (CCS1) in the plant nucleus and cytoplasm. Meanwhile, silencing CCS1 in SbCSD1-overexpressing plants revealed that SbCSD1 and CCS1 synergistically contribute to Cu stress tolerance. By integrating transcriptomic and genetic data, herein we provide novel insights into the orchestration of plant responses to heavy-metal stress in sorghum by SOD.
期刊介绍:
The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.