水稻中的小 RNA 生物发生受 MAP 激酶介导的 OsCDKD 磷酸化调控

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-09-16 DOI:10.1111/nph.20116

Dhanraj Singh, Neetu Verma, Balakrishnan Rengasamy, Gopal Banerjee, Alok Krishna Sinha

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引用次数: 0

摘要

摘要 CDK 是细胞分裂的主调节器，其活性受调节亚基细胞周期蛋白和 CAK 磷酸化的控制。然而，MAP激酶在调控植物细胞周期或CDKs中的作用尚未得到探讨。在这里，我们报告了 MAP 激酶 OsMPK3、OsMPK4 和 OsMPK6 与水稻中的 OsCDKD 及其调控亚基 OsCYCH 发生物理相互作用并磷酸化。MAP 激酶在 OsCDKD 的 Ser-168 和 Thr-235 残基上磷酸化 CDKD。MAP 激酶介导的 OsCDKD 磷酸化是其活化以控制小 RNA 生物发生所必需的。OsCDKD的磷酸化版本不能激活RNA聚合酶II的C端结构域，从而对小RNA的转录产生负面影响。此外，野生型（WT）OsCDKD 和磷酸化拟态 OsCDKD 的过表达株系与 WT、磷酸化拟态 OsCDKD-OE 和激酶凋亡 OsCDKD-OE 株系相比，根系生长、株高、分蘖数、圆锥花序数和种子数均有所增加。总之，我们的研究发现了水稻中由 MAPK 介导的磷酸化对 OsCDKD 的新型调控。MAPKs对OsCDKD的磷酸化通过调节miRNAs的转录对水稻的生长发育产生了积极的影响。

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The small RNA biogenesis in rice is regulated by MAP kinase-mediated OsCDKD phosphorylation

CDKs are the master regulator of cell division and their activity is controlled by the regulatory subunit cyclins and phosphorylation by the CAKs. However, the role of MAP kinases in regulating plant cell cycle or CDKs have not been explored.
Here, we report that the MAP kinases OsMPK3, OsMPK4, and OsMPK6 physically interact and phosphorylate OsCDKD and its regulatory subunit OsCYCH in rice. MAP kinases phosphorylate CDKD at Ser-168 and Thr-235 residues in OsCDKD. The MAP kinase-mediated phosphorylation of OsCDKD is required for its activation to control the small RNA biogenesis. The phosphodead version of OsCDKD fails to activate the C-terminal domain of RNA Polymerase II, thereby negatively impacting small RNA transcription.
Further, the overexpression lines of wild-type (WT) OsCDKD and phosphomimic OsCDKD show increased root growth, plant height, tiller number, panicle number, and seed number in comparison to WT, phosphodead OsCDKD-OE, and kinase-dead OsCDKD-OE plants.
In a nutshell, our study establishes a novel regulation of OsCDKD by MAPK-mediated phosphorylation in rice. The phosphorylation of OsCDKD by MAPKs imparts a positive effect on rice growth and development by regulating miRNAs transcription.

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.