低频正弦电磁场通过调节 miR-34b-5p/STAC2 促进大鼠骨髓间充质干细胞的成骨分化

IF 5.2 1区 生物学 Q1 BIOLOGY
Xuan Fang, Changyu Liu, Kang Wei, Zixing Shu, Yi Zou, Zihao Zhang, Qing Ding, Shaoze Jing, Weigang Li, Tianqi Wang, Hao Li, Hua Wu, Chaoxu Liu, Tian Ma
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引用次数: 0

摘要

电磁场已成为治疗骨质疏松症的一种有效方法。然而,其治疗功效的具体机制仍存在争议。在这里,我们证实了 15 Hz 和 0.4-1 mT 低频正弦波电磁场(SEMFs)对大鼠骨髓间充质干细胞(BMSCs)的促骨生成作用。随后的 miRNA 测序发现,在 0.4 mT 和 1 mT SEMFs 刺激组中,miR-34b-5p 均出现下调。为明确 miR-34b-5p 在成骨过程中的作用,分别用 miR-34b-5p 模拟物和抑制剂转染 BMSCs。结果表明,miR-34b-5p模拟物转染抑制成骨分化,而抑制miR-34b-5p则促进BMSCs的成骨分化。利用微型计算机断层扫描、H&E 染色和马森染色进行的体内评估表明,注射 miR-34b-5p 抑制剂可减轻卵巢切除术(OVX)大鼠的骨量损失和骨小梁微结构恶化。进一步的验证表明,miR-34b-5p 是通过调节 STAC2 的表达来发挥其作用的。调节 miR-34b-5p/STAC2 轴可减轻低频 SEMF 对 BMSCs 的促成骨作用。这些研究表明,SEMFs的促成骨作用部分是由于调控了miR-34b-5p/STAC2通路,这为骨质疏松症提供了一种潜在的候选疗法。低频正弦电磁场通过调节miR34b-5p/STAC2轴增强了小鼠的骨生成,为潜在的骨质疏松症治疗提供了启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Low frequency sinusoidal electromagnetic fields promote the osteogenic differentiation of rat bone marrow mesenchymal stem cells by modulating miR-34b-5p/STAC2

Low frequency sinusoidal electromagnetic fields promote the osteogenic differentiation of rat bone marrow mesenchymal stem cells by modulating miR-34b-5p/STAC2
Electromagnetic fields (EMFs) have emerged as an effective treatment for osteoporosis. However, the specific mechanism underlying their therapeutic efficacy remains controversial. Herein, we confirm the pro-osteogenic effects of 15 Hz and 0.4-1 mT low-frequency sinusoidal EMFs (SEMFs) on rat bone marrow mesenchymal stem cells (BMSCs). Subsequent miRNA sequencing reveal that miR-34b-5p is downregulated in both the 0.4 mT and 1 mT SEMFs-stimulated groups. To clarify the role of miR-34b-5p in osteogenesis, BMSCs are transfected separately with miR-34b-5p mimic and inhibitor. The results indicate that miR-34b-5p mimic transfection suppress osteogenic differentiation, whereas inhibition of miR-34b-5p promote osteogenic differentiation of BMSCs. In vivo assessments using microcomputed tomography, H&E staining, and Masson staining show that miR-34b-5p inhibitor injections alleviate bone mass loss and trabecular microstructure deterioration in ovariectomy (OVX) rats. Further validation demonstrates that miR-34b-5p exerts its effects by regulating STAC2 expression. Modulating the miR-34b-5p/STAC2 axis attenuate the pro-osteogenic effects of low-frequency SEMFs on BMSCs. These studies indicate that the pro-osteogenic effect of SEMFs is partly due to the regulation of the miR-34b-5p/STAC2 pathway, which provides a potential therapeutic candidate for osteoporosis. Low-frequency sinusoidal electromagnetic fields enhance osteogenesis in mice by regulating the miR34b-5p/STAC2 axis, providing insights into potential osteoporosis treatments.
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来源期刊
Communications Biology
Communications Biology Medicine-Medicine (miscellaneous)
CiteScore
8.60
自引率
1.70%
发文量
1233
审稿时长
13 weeks
期刊介绍: Communications Biology is an open access journal from Nature Research publishing high-quality research, reviews and commentary in all areas of the biological sciences. Research papers published by the journal represent significant advances bringing new biological insight to a specialized area of research.
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