以非病毒为靶向将大型有效载荷植入 T 细胞

IF 26.8 1区医学 Q1 ENGINEERING, BIOMEDICAL

Nature Biomedical Engineering Pub Date : 2024-09-16 DOI:10.1038/s41551-024-01252-0

Zsuzsanna Izsvák

引用次数: 0

摘要

利用核酸酶 Cas9 和 DNA 修复途径同源物介导的末端连接，可以高效、非病毒性地将大 DNA 有效载荷整合到原代 T 细胞的基因组靶位点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Non-viral targeted insertion of large payloads into T cells

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Non-viral targeted insertion of large payloads into T cells

The nuclease Cas9 and DNA-repair pathway homology-mediated end joining can be leveraged to efficiently and non-virally integrate large DNA payloads into genomic target sites in primary T cells.

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来源期刊

Nature Biomedical Engineering Medicine-Medicine (miscellaneous)

CiteScore

45.30

自引率

1.10%

发文量

138

期刊介绍： Nature Biomedical Engineering is an online-only monthly journal that was launched in January 2017. It aims to publish original research, reviews, and commentary focusing on applied biomedicine and health technology. The journal targets a diverse audience, including life scientists who are involved in developing experimental or computational systems and methods to enhance our understanding of human physiology. It also covers biomedical researchers and engineers who are engaged in designing or optimizing therapies, assays, devices, or procedures for diagnosing or treating diseases. Additionally, clinicians, who make use of research outputs to evaluate patient health or administer therapy in various clinical settings and healthcare contexts, are also part of the target audience.