将野生小鼠噬菌体移植到圈养家鼠体内后,肠道噬菌体群落趋同,而细菌群落不趋同

Dagmar Čížková, Pavel Payne, Anna Bryjová, Ľudovít Ďureje, Jaroslav Piálek, Jakub Kreisinger
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引用次数: 0

摘要

噬菌体是脊椎动物肠道微生物群落的重要组成部分,影响着细菌组的动态和进化,并直接与超级宿主相互作用。然而,关于自然条件下脊椎动物肠道噬菌体及其与细菌群相互作用的知识仅限于人类和非人灵长类动物。与野生小鼠相比,广泛使用的无特定病原体(SPF)小鼠宿主-微生物群相互作用模型的肠道细菌组发生了改变,而野生或其他非 SPF 小鼠的噬菌体数据却很缺乏。我们证明野生小鼠和人工饲养的非SPF小鼠的肠道噬菌体和细菌组存在差异,野生小鼠的噬菌体表现出更高的α多样性和个体间变异性。在这两组小鼠中,噬菌体组和细菌组的结构相互映照,在个体水平上相互关联。对 SPF 小鼠噬菌体组的先前数据进行重新分析后发现,与非 SPF 小鼠相比,SPF 小鼠富含 Suoliviridae crAss-like 噬菌体。小鼠模型中紊乱的细菌群可以通过移植健康的噬菌体来治疗,但噬菌体移植对健康成人肠道微生物群的影响仍然未知。我们的研究表明,将野生小鼠的噬菌体移植到人工饲养的小鼠体内不会导致受体噬菌体发生重大变化。然而,受体与供体噬菌体的融合证实了野生噬菌体可以整合到受体群落中。两个受体小鼠品系之间整合噬菌体子集的差异说明了噬菌体移植的影响取决于环境。移植对受体肠道细菌群没有影响。健康的成年肠道微生物组对干预的这种恢复能力对噬菌体异种移植的安全性具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Convergence of gut phage communities but not bacterial communities following wild mouse bacteriophage transplantation into captive house mice
Bacteriophages are abundant components of vertebrate gut microbial communities, impacting bacteriome dynamics, evolution, and directly interacting with the superhost. However, knowledge about gut phageomes and their interaction with bacteriomes in vertebrates under natural conditions is limited to humans and non-human primates. Widely used specific pathogen-free (SPF) mouse models of host-microbiota interactions have altered gut bacteriomes compared to wild mice, and data on phageomes from wild or other non-SPF mice are lacking. We demonstrate divergent gut phageomes and bacteriomes in wild and captive non-SPF mice, with wild mice phageomes exhibiting higher alpha-diversity and interindividual variability. In both groups, phageome and bacteriome structuring mirrored each other, correlating at the individual level. Re-analysis of previous data from phageomes of SPF mice revealed their enrichment in Suoliviridae crAss-like phages compared to our non-SPF mice. Disrupted bacteriomes in mouse models can be treated by transplanting healthy phageomes, but the effects of phageome transplants on healthy adult gut microbiota are still unknown. We show that experimental transplantation of phageomes from wild to captive mice did not cause major shifts in recipient phageomes. However, the convergence of recipient-to-donor phageomes confirmed that wild phages can integrate into recipient communities. The differences in the subset of integrated phages between the two recipient mouse strains illustrate the context-dependent effects of phage transplantation. The transplantation did not impact recipient gut bacteriomes. This resilience of healthy adult gut microbiomes to the intervention has implications for phage allotransplantation safety.
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