深海热液喷口代谢多能的氢弧菌对硫、氢和铁的氧化作用

Katja Laufer-Meiser, Malik Alawi, Stefanie Böhnke, Claus-Henning Solterbeck, Jana Schloesser, Axel Schippers, Philipp Dirksen, Thomas Brüser, Susann Henkel, Janina Fuss, Mirjam Perner
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引用次数: 0

摘要

在热液喷口,化学溶解自养型氢弧菌无处不在,数量也很多。它们能氧化硫、氢或铁,但没有一种能同时利用这三种能源。不过,这种能力在以高度动态的环境条件为特征的喷口中是非常有利的。我们从印度洋海脊沿岸的喷口中分离出了三种氢弧菌菌株,它们可以利用所有三种电子供体生长。我们展示了生长在铁、氢或硫代硫酸盐上的菌株的转录组数据,以及它们各自的氧化率和自养二氧化碳固定率、RubisCO 活性、扫描电镜和 EDX。铁、氢和硫代硫酸氧化作用对一株菌株氧化潜能的最大估计值分别为 10、24 和 952 毫摩尔,每通风口每小时的二氧化碳固定率分别为 0.3、1 和 84 毫摩尔,这表明它们与原位元素循环有关。根据所提供的无机电子供体的不同,一些基因上调或下调。虽然没有检测到已知的铁氧化基因,但上调的转录本表明存在铁获取和迄今未知的铁氧化途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Oxidation of sulfur, hydrogen, and iron by metabolically versatile Hydrogenovibrio from deep sea hydrothermal vents
Chemolithoautotrophic Hydrogenovibrio are ubiquitous and abundant at hydrothermal vents. They can oxidize sulfur, hydrogen or iron, but none are known to use all three energy sources. This ability though would be advantageous in vents hallmarked by highly dynamic environmental conditions. We isolated three Hydrogenovibrio strains from vents along the Indian Ridge, which grow on all three electron donors. We present transcriptomic data from strains grown on iron, hydrogen or thiosulfate with respective oxidation and autotrophic CO2 fixation rates, RubisCO activity, SEM, and EDX. Maximum estimates of one strain’s oxidation potential were 10, 24, and 952 mmol for iron, hydrogen and thiosulfate oxidation and 0.3, 1, and 84 mmol CO2 fixation, respectively, per vent per hour indicating their relevance for element cycling in-situ. Several genes were up- or downregulated depending on the inorganic electron donor provided. Although no known genes of iron-oxidation were detected, upregulated transcripts suggested iron-acquisition and so far unknown iron-oxidation-pathways.
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