PSIV-15 一过性胎牛病毒性腹泻病毒感染对产后生长、干物质消化率估计值、葡萄糖浓度和胴体特征的影响

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Alexandra C Miller, Huey Yi Loh, Erlene Chek, Octavio Guimaraes, Tyler A Thomas, Meghan P Thorndyke, Hana Van Campen, Jess N Kincade, Thomas R Hansen, Terry E Engle
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引用次数: 0

摘要

胎儿在妊娠 125 至 150 天前感染牛病毒性腹泻病毒(BVDV)会导致出生免疫耐受的持续感染(PI)犊牛。妊娠晚期感染 BVDV 会导致一过性胎儿感染(TI)。未接种 BVDV1 和 BVDV2 抗体血清阴性的一岁赫里福德小母牛(n = 25)通过人工授精与安格斯种公牛带有 X 染色体的精子进行配种,以研究 TI 对小母牛产后生长、干物质消化率、血糖浓度和胴体特征的影响。怀孕第 175 天,给小母牛鼻内接种 DMEM + 2% 马血清(假对照)以产生对照组小牛,或接种 4.0 log TCID50 非细胞病性 2 型 BVDV 以产生 TI 组小牛。所有假接种的对照母牛血清阴性,所有接种 BVDV 的母牛在接种后第 14 天血清转换。假接种对照组母鼠(n = 12)和 BVDV 接种母鼠(n = 12)均产下活犊。所有对照组的后代血清阴性,所有TI组的后代出生时2型BVDV抗体血清阳性。所有后代均在牧场饲养至断奶。断奶时,所有犊牛都被运到我们的饲养场研究设施,饲养在一组饲养栏中,并过渡到以高能量精饲料为主的日粮,直到体重达到约 600 千克。到达饲养场后,所有动物都接受了标准的小母牛生长植入物、含有 IBR-BRSV-PI3 的改良病毒活疫苗并进行了驱虫。在饲喂期的第 84 天,向 12 头年龄配对、单独饲喂的母牛(3 头对照组母牛和 3 头 TI 组母牛;约 1 岁)的日粮中添加二氧化钛,持续 28 天,用于估算干物质消化率。喂养约 280 天后,小母牛被运往美国农业部检查的屠宰场并收获。与对照组母牛相比,TI 母牛的出生体重(P & lt; 0.03)和最终体重(P & lt; 0.04)较轻。对照组母牛的平均日增重高于TI组母牛(P&P;lt; 0.01)。在所有采样时间点,对照组和TI组母牛的血糖浓度相似。单独喂养的小母牛的干物质摄入量在不同处理中相似。与对照组相比,TI 母牛的干物质消化率低 2.2% (P & lt; 0.05),热胴体重量轻 (P & lt; 0.01)。这些数据表明,TI胎儿BVDV感染可能通过影响胃肠道功能,对整个饲养期的生长产生负面影响。本研究得到了 USDA-NIFA Grant # 2019-67015-29866 的支持。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
PSIV-15 Effect of transient fetal bovine viral diarrhea virus infection on postnatal growth, estimated dry matter digestibility, glucose concentrations, and carcass characteristics
Fetal infection of bovine viral diarrhea virus (BVDV) before d 125 to 150 of gestation results in the birth of immunotolerant, persistently infected (PI) calves. Infection of BVDV during late gestation results in transient fetal infections (TI). Unvaccinated, yearling Hereford heifers (n = 25), seronegative for antibodies to BVDV1 and BVDV2, were bred by artificial insemination with X chromosome-bearing sperm from an Angus sire to examine the impact of TI on postnatal growth, estimated dry matter digestibility, blood glucose concentrations, and carcass characteristics. On d 175 of pregnancy, heifers were intranasally inoculated with either DMEM + 2% horse serum (sham control) to generate control calves or 4.0 log TCID50 noncytopathic type2 BVDV to generate TI calves. All sham-inoculated control dams remained seronegative, and all BVDV-inoculated dams seroconverted by d 14 post-inoculation. Sham-inoculated control dams (n = 12) and BVDV-inoculated dams (n = 12) gave birth to live calves. All control offspring were seronegative, and all TI offspring were seropositive for antibodies to type 2 BVDV at birth. All offspring were raised on pasture until weaning. At weaning, all calves were transported to our feedlot research facility, housed in one group feedlot pen, and transitioned to a high-energy concentrate-based diet until reaching an approximate BW of 600 kg. Upon arrival at the feedlot, all animals received a standard heifer growth implant, a modified live viral vaccine containing IBR-BRSV-PI3 and were dewormed. Heifer BW and jugular blood samples were collected every 28 d. On d 84 of the feeding period, titanium dioxide was added to the diet of 12, age-paired, individually fed, heifers (3 control and 3 TI heifers; approximately 1 yr of age) for 28 d and used to estimate dry matter digestibility. After approximately 280 d on feed heifers were transported to a USDA-inspected abattoir and harvested. The TI heifers had lighter birth weights (P < 0.03) and final BW (P < 0.04) when compared with control heifers. Average daily gain was greater (P < 0.01) in control compared with TI heifers. Blood glucose concentrations were similar between control and TI heifers at all sampling time points. Dry matter intake of individually fed heifers was similar across treatments. TI heifers had a 2.2% lesser (P < 0.05) dry matter digestibility and lighter (P < 0.01) hot carcass weights compared with controls. These data suggest that TI fetal BVDV infection negatively impacts growth throughout the feeding period, possibly by impacting gastrointestinal tract function. This research was supported by USDA-NIFA Grant # 2019-67015-29866.
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来源期刊
Journal of animal science
Journal of animal science 农林科学-奶制品与动物科学
CiteScore
4.80
自引率
12.10%
发文量
1589
审稿时长
3 months
期刊介绍: The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.
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