{"title":"分析阿尔及利亚食源性金黄色葡萄球菌分离物的毒力和耐药性基因特征","authors":"","doi":"10.1016/j.lwt.2024.116754","DOIUrl":null,"url":null,"abstract":"<div><p><em>Staphylococcus aureus</em>, a frequent commensal of the human microbiota, also frequently associated with various infections. This study aimed to genetically characterize foodborne methicillin resistant <em>S. aureus</em> (MRSA) in Oran, Algeria. From a total of 474 food matrices, 30 MRSA strains were isolated and characterized using whole genome sequencing (WGS) and bioinformatics. The genomes were assessed for antimicrobial resistance and virulence genes, sequence and <em>spa</em> types and single nucleotide polymorphism (SNP) to establish their relationship. The prevalence of <em>S. aureus</em> was 34.38% with MRSA accounting for 18.40%. MRSA strains showed high resistance to penicillinG, ampicillin and tetracycline. However, inconsistencies were noted between phenotypic and genotypic resistance for methicillin, aminoglycosides, and phenicolates, with <em>mecA</em> gene identified in three isolates. The isolates revealed eight different sequence types (predominantly ST97 and ST45) and eleven <em>spa</em> types (mainly t230 and t8503), correlating with SNP clusters except for isolates belonging to ST7200.86.66% of isolates harboured at least one enterotoxin gene underscores their potential public health threat. This investigation highlights the genetic heterogeneity among <em>S. aureus</em> regarding virulence and antimicrobial resistance, shedding light into food safety in Algeria and the ease of using WGS approach to rapidly characterize bacterial pathogens in community environments.</p></div>","PeriodicalId":382,"journal":{"name":"LWT - Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":6.0000,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0023643824010351/pdfft?md5=cefdaf8f7b06303ed3cc8510b459be8c&pid=1-s2.0-S0023643824010351-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Analysis of genetic signatures of virulence and resistance in foodborne Staphylococcus aureus isolates from Algeria\",\"authors\":\"\",\"doi\":\"10.1016/j.lwt.2024.116754\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Staphylococcus aureus</em>, a frequent commensal of the human microbiota, also frequently associated with various infections. This study aimed to genetically characterize foodborne methicillin resistant <em>S. aureus</em> (MRSA) in Oran, Algeria. From a total of 474 food matrices, 30 MRSA strains were isolated and characterized using whole genome sequencing (WGS) and bioinformatics. The genomes were assessed for antimicrobial resistance and virulence genes, sequence and <em>spa</em> types and single nucleotide polymorphism (SNP) to establish their relationship. The prevalence of <em>S. aureus</em> was 34.38% with MRSA accounting for 18.40%. MRSA strains showed high resistance to penicillinG, ampicillin and tetracycline. However, inconsistencies were noted between phenotypic and genotypic resistance for methicillin, aminoglycosides, and phenicolates, with <em>mecA</em> gene identified in three isolates. The isolates revealed eight different sequence types (predominantly ST97 and ST45) and eleven <em>spa</em> types (mainly t230 and t8503), correlating with SNP clusters except for isolates belonging to ST7200.86.66% of isolates harboured at least one enterotoxin gene underscores their potential public health threat. This investigation highlights the genetic heterogeneity among <em>S. aureus</em> regarding virulence and antimicrobial resistance, shedding light into food safety in Algeria and the ease of using WGS approach to rapidly characterize bacterial pathogens in community environments.</p></div>\",\"PeriodicalId\":382,\"journal\":{\"name\":\"LWT - Food Science and Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.0000,\"publicationDate\":\"2024-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0023643824010351/pdfft?md5=cefdaf8f7b06303ed3cc8510b459be8c&pid=1-s2.0-S0023643824010351-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"LWT - Food Science and Technology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0023643824010351\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"LWT - Food Science and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0023643824010351","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Analysis of genetic signatures of virulence and resistance in foodborne Staphylococcus aureus isolates from Algeria
Staphylococcus aureus, a frequent commensal of the human microbiota, also frequently associated with various infections. This study aimed to genetically characterize foodborne methicillin resistant S. aureus (MRSA) in Oran, Algeria. From a total of 474 food matrices, 30 MRSA strains were isolated and characterized using whole genome sequencing (WGS) and bioinformatics. The genomes were assessed for antimicrobial resistance and virulence genes, sequence and spa types and single nucleotide polymorphism (SNP) to establish their relationship. The prevalence of S. aureus was 34.38% with MRSA accounting for 18.40%. MRSA strains showed high resistance to penicillinG, ampicillin and tetracycline. However, inconsistencies were noted between phenotypic and genotypic resistance for methicillin, aminoglycosides, and phenicolates, with mecA gene identified in three isolates. The isolates revealed eight different sequence types (predominantly ST97 and ST45) and eleven spa types (mainly t230 and t8503), correlating with SNP clusters except for isolates belonging to ST7200.86.66% of isolates harboured at least one enterotoxin gene underscores their potential public health threat. This investigation highlights the genetic heterogeneity among S. aureus regarding virulence and antimicrobial resistance, shedding light into food safety in Algeria and the ease of using WGS approach to rapidly characterize bacterial pathogens in community environments.
期刊介绍:
LWT - Food Science and Technology is an international journal that publishes innovative papers in the fields of food chemistry, biochemistry, microbiology, technology and nutrition. The work described should be innovative either in the approach or in the methods used. The significance of the results either for the science community or for the food industry must also be specified. Contributions written in English are welcomed in the form of review articles, short reviews, research papers, and research notes. Papers featuring animal trials and cell cultures are outside the scope of the journal and will not be considered for publication.