微孢子虫-杯状病毒在同时感染树木落叶虫西伯利亚斛(鳞翅目:褐翅蝉科)过程中的相互作用

IF 3.6 3区 生物学 Q1 ZOOLOGY
Arina S. Rumiantseva , Aleksander A. Ageev , Anastasia N. Ignatieva , Maria E. Yakimova , Daria D. Kharlamova , Vyacheslav V. Martemyanov , Yuri S. Tokarev
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引用次数: 0

摘要

西伯利亚蛾(Dendrolimus sibiricus)是一种危险的森林落叶害虫,是亚洲北方森林的头号害虫。为寻求有效和生态友好的控制措施,人们开始关注微生物病原体。病毒和微孢子虫是广泛存在于昆虫种群中的细胞内寄生虫,可引起慢性或急性感染。这些病原体之间的相互作用从拮抗到协同不等。这项工作的目的是通过喂食接种体(病毒多面体和微孢子虫孢子),测试最近从西伯利亚蝇中分离出的细胞质多面体病毒(细胞病毒)株 DsCPV-1 与微孢子虫对西伯利亚蝇的作用。对寄生于鳞翅目昆虫的三种不同的微孢子虫进行了单感染 D. sibiricus 的试验:家蚕的 Nosema bombycis、玉米螟的 N. pyrausta 和甜菜网虫的 Tubulinosema loxostegi。Nosema bombycis 的毒力最强,用 10 万和 100 万个孢子/幼虫处理第一和第二龄幼虫时,中位致死时间分别为 7 天。选择 Nosema bombycis(剂量为 10 万个孢子/幼虫)与极低剂量的 DsCPV-1(1 个多面体/幼虫)混合感染,对两种西伯利亚鹅掌楸二龄幼虫(杉木取食种和落叶松取食种)进行试验。混合感染对幼虫致死时间和体重的负面影响最为显著。混合感染对以杉木为食的幼虫有协同效应,但对以落叶松为食的幼虫只有叠加效应。两种病原体以相同的接种量比例成功地在幼虫体内共同繁殖。因此,这些昆虫病原体的组合有望用于森林保护,防止西伯利亚夜蛾的危害,也是大幅减少田间病原体用量的一种方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Microsporidia-cypovirus interactions during simultaneous infection of the tree defoliator Dendrolimus sibiricus (Lepidoptera: Lasiocampidae)

Microsporidia-cypovirus interactions during simultaneous infection of the tree defoliator Dendrolimus sibiricus (Lepidoptera: Lasiocampidae)

The Siberian moth, Dendrolimus sibiricus is a dangerous forest defoliator, the number one pest of boreal forests in Asia. Search for effective and ecologically friendly control measures drives attention to microbial pathogens. Viruses and microsporidia are obligate intracellular parasites widespread in insect populations causing either chronic or acute infections. Interactions of these pathogens vary from antagonistic to synergistic. The goal of the work was to test a recently discovered cytoplasmatic polyhedrosis virus (cypovirus) strain DsCPV-1 isolated from D.sibiricus, combined with a microsporidium, against D. sibiricus, by feeding the inoculum (viral polyhedral and microsporidian spores). Three different microsporidian parasites of lepidopterans were tested against D. sibiricus as monoinfection: Nosema bombycis from silkworm, N. pyrausta from corn borer, and Tubulinosema loxostegi from beet webworm. Nosema bombycis was the most virulent, with a median lethal time of 7 days in the first and second instars treated with 100,000 and 1 million spores/larva, respectively. Nosema bombycis (dose 100,000 spores/larva) was chosen to test it as mixed infection in combination with an extremely low dose of DsCPV-1 (1 polyhedron/larva) against two races of D. sibiricus second instar larvae (the fir-feeding race and the larch-feeding race). The mixed infection demonstrated the most prominent negative effect on larval lethal time and weight for the both tested races. Mixed infections showed a synergistic effect for the fir-feeding larvae but additive effect only for the larch feeding larvae. Both pathogens co-developed successfully in the larvae with equal ratio of producing inoculum. The combination of these entomopathogens is therefore promising for forest protection against the Siberian moth and could be the way to significantly decrease the amount of pathogens applied in field.

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来源期刊
CiteScore
6.10
自引率
5.90%
发文量
94
审稿时长
1 months
期刊介绍: The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.
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