{"title":"微孢子虫-杯状病毒在同时感染树木落叶虫西伯利亚斛(鳞翅目:褐翅蝉科)过程中的相互作用","authors":"","doi":"10.1016/j.jip.2024.108199","DOIUrl":null,"url":null,"abstract":"<div><p>The Siberian moth, <em>Dendrolimus sibiricus</em> is a dangerous forest defoliator, the number one pest of boreal forests in Asia. Search for effective and ecologically friendly control measures drives attention to microbial pathogens. Viruses and microsporidia are obligate intracellular parasites widespread in insect populations causing either chronic or acute infections. Interactions of these pathogens vary from antagonistic to synergistic. The goal of the work was to test a recently discovered cytoplasmatic polyhedrosis virus (cypovirus) strain DsCPV-1 isolated from <em>D.sibiricus</em>, combined with a microsporidium, against <em>D. sibiricus,</em> by feeding the inoculum (viral polyhedral and microsporidian spores)<em>.</em> Three different microsporidian parasites of lepidopterans were tested against <em>D. sibiricus</em> as monoinfection: <em>Nosema bombycis</em> from silkworm<em>, N. pyrausta</em> from corn borer<em>,</em> and <em>Tubulinosema loxostegi</em> from beet webworm. <em>Nosema bombycis</em> was the most virulent, with a median lethal time of 7 days in the first and second instars treated with 100,000 and 1 million spores/larva, respectively. <em>Nosema bombycis</em> (dose 100,000 spores/larva) was chosen to test it as mixed infection in combination with an extremely low dose of DsCPV-1 (1 polyhedron/larva) against two races of <em>D. sibiricus</em> second instar larvae (the fir-feeding race and the larch-feeding race). The mixed infection demonstrated the most prominent negative effect on larval lethal time and weight for the both tested races. Mixed infections showed a synergistic effect for the fir-feeding larvae but additive effect only for the larch feeding larvae. Both pathogens co-developed successfully in the larvae with equal ratio of producing inoculum. The combination of these entomopathogens is therefore promising for forest protection against the Siberian moth and could be the way to significantly decrease the amount of pathogens applied in field.</p></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Microsporidia-cypovirus interactions during simultaneous infection of the tree defoliator Dendrolimus sibiricus (Lepidoptera: Lasiocampidae)\",\"authors\":\"\",\"doi\":\"10.1016/j.jip.2024.108199\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The Siberian moth, <em>Dendrolimus sibiricus</em> is a dangerous forest defoliator, the number one pest of boreal forests in Asia. Search for effective and ecologically friendly control measures drives attention to microbial pathogens. Viruses and microsporidia are obligate intracellular parasites widespread in insect populations causing either chronic or acute infections. Interactions of these pathogens vary from antagonistic to synergistic. The goal of the work was to test a recently discovered cytoplasmatic polyhedrosis virus (cypovirus) strain DsCPV-1 isolated from <em>D.sibiricus</em>, combined with a microsporidium, against <em>D. sibiricus,</em> by feeding the inoculum (viral polyhedral and microsporidian spores)<em>.</em> Three different microsporidian parasites of lepidopterans were tested against <em>D. sibiricus</em> as monoinfection: <em>Nosema bombycis</em> from silkworm<em>, N. pyrausta</em> from corn borer<em>,</em> and <em>Tubulinosema loxostegi</em> from beet webworm. <em>Nosema bombycis</em> was the most virulent, with a median lethal time of 7 days in the first and second instars treated with 100,000 and 1 million spores/larva, respectively. <em>Nosema bombycis</em> (dose 100,000 spores/larva) was chosen to test it as mixed infection in combination with an extremely low dose of DsCPV-1 (1 polyhedron/larva) against two races of <em>D. sibiricus</em> second instar larvae (the fir-feeding race and the larch-feeding race). The mixed infection demonstrated the most prominent negative effect on larval lethal time and weight for the both tested races. Mixed infections showed a synergistic effect for the fir-feeding larvae but additive effect only for the larch feeding larvae. Both pathogens co-developed successfully in the larvae with equal ratio of producing inoculum. The combination of these entomopathogens is therefore promising for forest protection against the Siberian moth and could be the way to significantly decrease the amount of pathogens applied in field.</p></div>\",\"PeriodicalId\":16296,\"journal\":{\"name\":\"Journal of invertebrate pathology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2024-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of invertebrate pathology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022201124001423\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of invertebrate pathology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022201124001423","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}
Microsporidia-cypovirus interactions during simultaneous infection of the tree defoliator Dendrolimus sibiricus (Lepidoptera: Lasiocampidae)
The Siberian moth, Dendrolimus sibiricus is a dangerous forest defoliator, the number one pest of boreal forests in Asia. Search for effective and ecologically friendly control measures drives attention to microbial pathogens. Viruses and microsporidia are obligate intracellular parasites widespread in insect populations causing either chronic or acute infections. Interactions of these pathogens vary from antagonistic to synergistic. The goal of the work was to test a recently discovered cytoplasmatic polyhedrosis virus (cypovirus) strain DsCPV-1 isolated from D.sibiricus, combined with a microsporidium, against D. sibiricus, by feeding the inoculum (viral polyhedral and microsporidian spores). Three different microsporidian parasites of lepidopterans were tested against D. sibiricus as monoinfection: Nosema bombycis from silkworm, N. pyrausta from corn borer, and Tubulinosema loxostegi from beet webworm. Nosema bombycis was the most virulent, with a median lethal time of 7 days in the first and second instars treated with 100,000 and 1 million spores/larva, respectively. Nosema bombycis (dose 100,000 spores/larva) was chosen to test it as mixed infection in combination with an extremely low dose of DsCPV-1 (1 polyhedron/larva) against two races of D. sibiricus second instar larvae (the fir-feeding race and the larch-feeding race). The mixed infection demonstrated the most prominent negative effect on larval lethal time and weight for the both tested races. Mixed infections showed a synergistic effect for the fir-feeding larvae but additive effect only for the larch feeding larvae. Both pathogens co-developed successfully in the larvae with equal ratio of producing inoculum. The combination of these entomopathogens is therefore promising for forest protection against the Siberian moth and could be the way to significantly decrease the amount of pathogens applied in field.
期刊介绍:
The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates.
The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.