用于输送羊膜间充质干细胞代谢物产品的可溶解微针贴片,促进紫外线老化诱导小鼠的皮肤再生

IF 4.4 2区 医学 Q1 PHARMACOLOGY & PHARMACY
Andang Miatmoko , Berlian Sarasitha Hariawan , Devy Maulidya Cahyani , Qonita Kurnia Anjani , Febri Annuryanti , Rifda Tarimi Octavia , Djoko Legowo , Kusuma Eko Purwantari , Noorma Rosita , Purwati , Ryan F. Donnelly , Dewi Melani Hariyadi
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引用次数: 0

摘要

羊膜间充质干细胞代谢产物(AMSC-MP)含有高分子量的亲水性蛋白质成分,微针为增强其真皮输送提供了一种前景广阔的解决方案,从而达到嫩肤和改善人类健康的目的。本研究旨在评估AMSC-MP微针贴片的理化特性和在紫外线老化诱导的小鼠体内有效再生皮肤组织的功效。研究人员采用双层浇注法制作了由分子量为 9-10 kDa 的聚乙烯醇和分子量为 56 kDa 的聚乙烯吡咯烷酮组成的可溶解微针贴片,AMSC-MP 的浓度分别为 30% (MN30)、25% (MN25) 和 20% (MN20)。然后对微针贴片的形态、机械阻力和插入性能进行了评估。此外,还使用弗兰茨细胞法进行了体内外释放研究,并通过胶原密度评分、成纤维细胞计数和紫外线老化诱导小鼠皮肤刺激性研究确定了体内疗效和刺激性。AMSC-MP 微针呈金字塔形,针尖锋利 500 微米。机械测试显示,MN30 最深插入 Parafilm® M(447.44 ± 37.21 µm),而 MN25 最深插入全厚猪皮(717.92 ± 25.40 µm)。研究显示,MN20 的 EGF 释放受控时间长达 24 小时,沉积率最高(55.94 ± 12.34 %)。这些研究结果表明,微针能成功穿透角质层和有活力的表皮。所有微针配方的胶原密度评分和成纤维细胞计数都明显高于对照组,其中 MN30 的数值最高。炎症细胞计数显示,在体内研究中,炎症细胞极少,表明无刺激性。可溶解微针贴片具有良好的特性,能有效地输送 AMSC-MP,刺激性极低,为输送生物抗衰老剂促进皮肤再生提供了潜在的技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Dissolving microneedle patches for delivery of amniotic mesenchymal stem cell metabolite products for skin regeneration in UV-aging induced mice

Dissolving microneedle patches for delivery of amniotic mesenchymal stem cell metabolite products for skin regeneration in UV-aging induced mice

Microneedles offer a promising solution to enhancing dermal delivery of amniotic mesenchymal stem cell metabolite product (AMSC-MP), which contains hydrophilic protein components with high molecular weight, for the purposes of skin rejuvenation and improving human health. This study aimed to evaluate the physicochemical characteristics and in vivo efficacy of AMSC-MP-loaded microneedle patches for effectively regenerating skin tissues in UV-aging induced mice. Dissolving microneedle patches, composed of polyvinyl alcohol with an MW of 9–10 kDa and polyvinylpyrrolidone with an MW of 56 kDa, were fabricated using the double-casting method at three AMSC-MP concentrations: i.e., 30 % (MN30), 25 % (MN25), and 20 % (MN20). The microneedles patches were then evaluated for morphological, mechanical resistance, and insertion properties. An ex vivo release study was also conducted using the Franz cell method, and in vivo efficacy and irritation were then determined through collagen density scores, fibroblast cell counts, and skin irritation studies of UV-aging induced mice. The AMSC-MP microneedles displayed a pyramidal shape with 500 µm sharp tips. Mechanical testing revealed that MN30 achieved its deepest insertion into Parafilm® M (447.44 ± 37.21 µm), while MN25 achieved its deepest insertion into full-thickness porcine skin (717.92 ± 25.40 µm). The study revealed a controlled EGF release for up to 24 h, with MN20 exhibiting the highest deposition (55.94 ± 12.34 %). These findings demonstrate the successful penetration of microneedles through the stratum corneum and viable epidermis. Collagen density scores and fibroblast cell counts were significantly higher in all microneedle formulations than the control, with MN30 having the highest values. Inflammatory cell counts indicated minimal presence suggesting non-irritation in the in vivo study. Dissolving microneedle patches exhibited favorable characteristics and efficiently delivered AMSC-MP with minimal potential for irritation, providing potential technology for delivering biological anti-aging agents for the purposes of fostering skin regeneration.

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来源期刊
CiteScore
8.80
自引率
4.10%
发文量
211
审稿时长
36 days
期刊介绍: The European Journal of Pharmaceutics and Biopharmaceutics provides a medium for the publication of novel, innovative and hypothesis-driven research from the areas of Pharmaceutics and Biopharmaceutics. Topics covered include for example: Design and development of drug delivery systems for pharmaceuticals and biopharmaceuticals (small molecules, proteins, nucleic acids) Aspects of manufacturing process design Biomedical aspects of drug product design Strategies and formulations for controlled drug transport across biological barriers Physicochemical aspects of drug product development Novel excipients for drug product design Drug delivery and controlled release systems for systemic and local applications Nanomaterials for therapeutic and diagnostic purposes Advanced therapy medicinal products Medical devices supporting a distinct pharmacological effect.
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