427 妊娠晚期缓释富氮日粮对小牛骨骼肌能量代谢的影响:蛋白质组和转录组方法

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Mateus P Gionbelli, Thais C Costa, Diana C Cediel-Devia, Karolina B Nascimento, Tathyane R S Gionbelli, Marcio S Duarte
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引用次数: 0

摘要

本研究旨在通过蛋白质组和转录组数据,确定妊娠晚期母体缓释氮日粮相关的丰富生物过程及其对后代骨骼肌的影响。从妊娠 180 天到 268 天,16 头妊娠婆罗门奶牛被分为两组:对照组(CON,n = 12)为低粗蛋白质(CP)基础日粮(6% CP,自由采食)加矿物质混合物(130 gּ牛-1ּd-1),CON 处理组为添加缓释 N(SRN,n = 9)的蛋白质精料、n = 9)为基础的蛋白质精料补充剂[40% CP,每公斤体重(BW)/日早晨饲喂 2 克],由玉米、豆粕、尿素和 SRN 源(Timafeed Boost,Roullier 集团,法国圣马洛)组成。在小牛45日龄时进行肌肉活检,以进行RNA测序(RNA-seq)和蛋白质组(HPLC-MS/MS)分析。使用 FASTQc 软件评估 RNAseq 原始数据的质量控制,并使用 Trimmomatic 对低质量读数进行修剪。使用 STAR 将读数与金牛参考基因组进行映射。使用 R 环境中的 limma 软件包和 Cuffdiff 工具分别鉴定了调整后 P 值≤ 0.05 的差异表达基因和同工酶。蛋白质组数据在 MaxQuant 中根据金牛参考蛋白质组进行处理,并使用 R 环境中的 MSqRob 软件包进行统计分析。值得注意的是,虽然实验处理并不影响转录本丰度,但却观察到了蛋白质水平的差异。SRN组小牛骨骼肌中丰富的生物过程包括丙酮酸乙酰-CoA的生物合成、线粒体呼吸链复合物的组装、钙离子跨膜转运的调控以及与能量和氮代谢有关的其他一些过程。蛋白质-蛋白质相互作用网络分析揭示了 ATP 和葡萄糖代谢、三羧酸循环和肌节组织等关键过程。总之,我们的研究结果强调了妊娠晚期缓释富氮饮食对小牛骨骼肌能量代谢的有益影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
427 Impact of late gestation slow-release nitrogen-enriched diets on energy metabolism in calf skeletal muscle: A proteomic and transcriptomic approach
The current study aimed to determine the enriched biological processes, through proteomic and transcriptomic data, associated with maternal slow-release nitrogen diets received during late gestation and their effects on the skeletal muscle of offspring. From d 180 to d 268 of gestation, a total of 16 pregnant Brahman cows were divided into two groups: a control (CON, n = 12) low crude protein (CP) basal diet (6% of CP, ad libitum) plus mineral mixture (130 gּ cow-1ּ d-1), or the CON treatment supplemented with a slow-released N (SRN, n = 9) based protein concentrate supplement [40% CP, fed 2 g/kg of body weight (BW)/d in the morning) composed by corn, soybean meal, urea and a SRN source (Timafeed Boost, Roullier Group, Saint-Malo, France). Muscle biopsies were performed on d 45 of age in calves for RNA sequencing (RNA-seq) and proteomic (HPLC-MS/MS) analyses. The quality control of RNAseq raw data was assessed using FASTQc software, with low-quality reads trimmed by Trimmomatic. Reads were mapped against the Bos taurus reference genome using STAR. Differentially expressed (DE) genes and isoforms, significant at an adjusted P-value ≤ 0.05, were identified using the limma package and Cuffdiff tool, respectively, in the R environment. Proteomic data was processed in MaxQuant against the Bos taurus reference proteome, with statistical analysis performed using the MSqRob package in R. Network analyses identified significant biological processes (adjusted P-value ≤ 0.05) among differentially abundant proteins (DAPs). Notably, while the experimental treatment did not affect transcript abundance, protein-level differences were observed. Enriched biological processes in SRN group skeletal muscle of calves included acetyl-CoA biosynthesis from pyruvate, mitochondrial respiratory chain complex assembly, regulation of calcium ion transmembrane transport, and several others related to energy and nitrogen metabolism. Protein-protein interaction network analyses revealed key processes such as ATP and glucose metabolism, tricarboxylic acid cycle, and sarcomere organization. Overall, our findings underscore the beneficial impact of slow-release nitrogen-enriched diets during late gestation on energy metabolism in calf skeletal muscle.
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来源期刊
Journal of animal science
Journal of animal science 农林科学-奶制品与动物科学
CiteScore
4.80
自引率
12.10%
发文量
1589
审稿时长
3 months
期刊介绍: The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.
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