城市污水处理厂的活性污泥絮凝物中棒状杆菌与 Zoogloea 之间独特的共生关系

IF 3.6 4区 生物学 Q2 ENVIRONMENTAL SCIENCES
Naoki Fujii, Kyohei Kuroda, Takashi Narihiro, Yoshiteru Aoi, Noriatsu Ozaki, Akiyoshi Ohashi, Tomonori Kindaichi
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引用次数: 0

摘要

拟杆菌(又称候选菌群辐射(CPR)),包括类级未培养支系 JAEDAM01(原为 Gracilibacteria/GN02/BD1-5 的一个亚类),在活性污泥中无处不在。然而,它们的特征以及与其他生物的关系在很大程度上还不为人所知。据信,它们是共生、内共生或捕食性的。尽管我们了解到它们的代谢能力有限,但由于难以培养和识别,它们的确切作用仍然难以捉摸。在之前的研究中,我们成功地从活性污泥絮凝物中回收了高质量的元基因组组装基因组(MAGs),其中包括 JAEDAM01 的一个成员。在这项研究中,我们设计了新的探针来观察与 JAEDAM01 相关的目标 MAG HHAS10,并利用荧光原位杂交(FISH)鉴定了其宿主。FISH 观察发现,JAEDAM01 HHAS10 样细胞位于密集的 Zoogloea 细胞簇中,在 CPR 细胞附近,Zoogloeal 细胞的荧光亮度降低。Zoogloea MAGs拥有与细胞外高分子物质生物合成、絮状物形成和营养物质清除有关的基因,包括聚羟基烷酸(PHA)积累途径。JAEDAM01 MAG HHAS10拥有与IV型纤毛、能力蛋白EC和PHA降解相关的基因,这表明活性污泥絮团中的生活方式依赖于Zoogloea。这些发现表明 JAEDAM01 与 Zoogloea 之间存在一种新的共生关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Unique episymbiotic relationship between Candidatus Patescibacteria and Zoogloea in activated sludge flocs at a municipal wastewater treatment plant

Unique episymbiotic relationship between Candidatus Patescibacteria and Zoogloea in activated sludge flocs at a municipal wastewater treatment plant

Candidatus Patescibacteria, also known as candidate phyla radiation (CPR), including the class-level uncultured clade JAEDAM01 (formerly a subclass of Gracilibacteria/GN02/BD1-5), are ubiquitous in activated sludge. However, their characteristics and relationships with other organisms are largely unknown. They are believed to be episymbiotic, endosymbiotic or predatory. Despite our understanding of their limited metabolic capacity, their precise roles remain elusive due to the difficulty in cultivating and identifying them. In previous research, we successfully recovered high-quality metagenome-assembled genomes (MAGs), including a member of JAEDAM01 from activated sludge flocs. In this study, we designed new probes to visualize the targeted JAEDAM01-associated MAG HHAS10 and identified its host using fluorescence in situ hybridization (FISH). The FISH observations revealed that JAEDAM01 HHAS10-like cells were located within dense clusters of Zoogloea, and the fluorescence brightness of zoogloeal cells decreased in the vicinity of the CPR cells. The Zoogloea MAGs possessed genes related to extracellular polymeric substance biosynthesis, floc formation and nutrient removal, including a polyhydroxyalkanoate (PHA) accumulation pathway. The JAEDAM01 MAG HHAS10 possessed genes associated with type IV pili, competence protein EC and PHA degradation, suggesting a Zoogloea-dependent lifestyle in activated sludge flocs. These findings indicate a new symbiotic relationship between JAEDAM01 and Zoogloea.

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来源期刊
Environmental Microbiology Reports
Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY
CiteScore
6.00
自引率
3.00%
发文量
91
审稿时长
3.0 months
期刊介绍: The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.
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