UFMylation 参与血清炎症细胞因子的生成和脂多糖诱导的脾 T 细胞活化

IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
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引用次数: 0

摘要

UFMylation 是一种新型泛素样蛋白修饰系统,最近被发现在炎症中被激活。然而,UFMylation 激活对体内炎症的影响仍不清楚。在本研究中,我们利用转基因(TG)技术产生了一种 UFMylation 激活小鼠。用脂多糖(LPS)诱导 TG 和非转基因(NTG)小鼠的全身炎症。使用小鼠细胞因子阵列检测血清细胞因子,并使用流式细胞术测定脾脏 NK、B 和 T 细胞的比例。我们发现,与基线时的 NTG 小鼠相比,TG 小鼠的血清 G-CSF、TNF RII 增加,而血清 TCA-3、CD30L、bFGF、IL-15 和 MIG 减少。此外,与 NTG 小鼠相比,TG 小鼠的血清细胞因子对 LPS 的反应也不同。LPS 上调血清 TNF RII、G-CSF、MCP-5、RANTES、KC、BLC、MIG,下调 IL-1b、IL-2、IL-3、IL-4、IL-5、IL-7、IL-10、IL-12p40、IL-15、IL-17、IFN-γ、TCA-3、Eotaxin-2、LIX、MCP-1、TNFα、而在 TG 小鼠中,LPS 上调 G-CSF、MCP-5、RANTES、KC、BLC、MIG、ICAM-1、PF4、Eotaxin、CD30L、MIP-1a、TNFRI,下调 IL-1b、IL-3、LIX、MCP-1、TNFα、GM-CSF。流式细胞术数据表明,LPS 显著降低了 NTG 小鼠 NK 和 NKT 细胞的百分比,而 UFMylation 激活则抑制了 LPS 诱导的 NKT 细胞减少。TG和NTG小鼠的B细胞、总CD4+和总CD8+T细胞比例对LPS处理的反应相当,而CD4+CD69+和CD8+CD69+T细胞的百分比在TG小鼠中较低。这些发现表明,UFMylation 可能会改变 LPS 诱导的血清细胞因子谱,并参与体内脾 T 细胞的活化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
UFMylation is involved in serum inflammatory cytokines generation and splenic T cell activation induced by lipopolysaccharide

UFMylation, a novel ubiquitin-like protein modification system, has been recently found to be activated in inflammation. However, the effects of UFMylation activation on inflammation in vivo remains unclear. In the present study, we generated a UFMylation activated mice using transgenic (TG) techniques. Lipopolysaccharide (LPS) was used to induce systemic inflammation in both TG and non-transgenic (NTG) mice. Serum cytokines were detected using a Mouse Cytokine Array, and the proportions of splenic NK, B and T cells were determined by using flow cytometry. We found that TG mice showed increased serum G-CSF, TNF RII and decreased serum TCA-3, CD30L, bFGF, IL-15 and MIG compared with NTG mice at baseline. Furthermore, serum cytokines in TG mice exhibited different responses to LPS compared to NTG mice. LPS up-regulated serum TNF RII, G-CSF, MCP-5, RANTES, KC, BLC, MIG and down-regulated IL-1b, IL-2, IL-3, IL-4, IL-5, IL-7, IL-10, IL-12p40, IL-15, IL-17, IFN-γ, TCA-3, Eotaxin-2, LIX, MCP-1, TNFα, GM-CSF in NTG mice, whereas LPS up-regulated G-CSF, MCP-5, RANTES, KC, BLC, MIG, ICAM-1, PF4, Eotaxin, CD30L, MIP-1a, TNFRI and down-regulated IL-1b, IL-3, LIX, MCP-1, TNFα, GM-CSF in TG mice. Data from flow cytometry indicated that LPS significantly reduced the percentages of NK and NKT cells in NTG mice, whereas UFMylation activation inhibited LPS-induced NKT cell decrease. The proportions of B cells, total CD4+ and total CD8+ T cells were comparable between TG and NTG mice in response to LPS treatment, whereas the percentages of CD4+CD69+ and CD8+CD69+T cells were lower in TG mice. These findings suggest that UFMylation may alter LPS-induced serum cytokine profile and participate in splenic T cell activation in vivo.

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来源期刊
Cytokine
Cytokine 医学-免疫学
CiteScore
7.60
自引率
2.60%
发文量
262
审稿时长
48 days
期刊介绍: The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.
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