世界卫生组织 HPV6 HPV11 HPV31 HPV33 HPV45 HPV52 和 HPV58 抗体国际标准

IF 6.9 1区 医学 Q1 IMMUNOLOGY
Troy J. Kemp, Gitika Panicker, Carina Eklund, Jianhui Nie, Youchun Wang, Simon Beddows, Peter Rigsby, Weijin Huang, Joakim Dillner, Elizabeth R. Unger, Ligia A. Pinto, Dianna E. Wilkinson
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引用次数: 0

摘要

世界卫生组织(WHO)之前制定的抗HPV16和HPV18抗体国际标准(IS)用于统一人类乳头瘤病毒(HPV)血清学检测的结果。在此,我们介绍了一项国际合作研究,旨在建立抗 HPV6(NIBSC 代码 19/298)、HPV11(20/174)、HPV31(20/176)、HPV33(19/290)、HPV45(20/178)、HPV52(19/296)和 HPV58(19/300)抗体的国际标准。候选标准品是用自然感染者的血清制备的。除了 HPV11 候选标准品对其他类型的 HPV 也有反应外,每个候选标准品对其指定的 HPV 类型都有单特异性反应。相对于相关候选 IS 的抗体水平的表达减少了实验室之间的差异,从而提高了实验室之间的可比性。根据这些结果,世卫组织生物标准化专家委员会将这 7 种候选抗血清中的每一种都确定为其所针对的 HPV 类型的第一 IS,用于基于假病毒的 HPV 中和与抗体结合测定的标准化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

WHO International Standards for antibodies to HPV6 HPV11 HPV31 HPV33 HPV45 HPV52 and HPV58

WHO International Standards for antibodies to HPV6 HPV11 HPV31 HPV33 HPV45 HPV52 and HPV58

Previously established World Health Organization (WHO) International Standards (IS) for anti-HPV16 and HPV18 antibodies are used to harmonize results across human papillomavirus (HPV) serology assays. Here, we present an international collaborative study to establish ISs for antibodies against HPV6 (NIBSC code 19/298), HPV11 (20/174), HPV31 (20/176), HPV33 (19/290), HPV45 (20/178), HPV52 (19/296) and HPV58 (19/300). The candidate standards were prepared using sera from naturally infected individuals. Each candidate was shown to be monospecific for reactivity against its indicated HPV type except for the HPV11 candidate, which was also reactive against other types. Expression of antibody levels relative to the relevant candidate IS reduced inter-laboratory variation allowing greater comparability between laboratories. Based on these results, the WHO Expert Committee on Biological Standardization established each of the 7 candidates as the 1st IS for antiserum to its indicated HPV type for use in the standardization of HPV pseudovirion-based neutralization and antibody-binding assays.

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来源期刊
NPJ Vaccines
NPJ Vaccines Immunology and Microbiology-Immunology
CiteScore
11.90
自引率
4.30%
发文量
146
审稿时长
11 weeks
期刊介绍: Online-only and open access, npj Vaccines is dedicated to highlighting the most important scientific advances in vaccine research and development.
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