Xuan Shi, Yanfang Liu, Teng Ma, Hao Jin, Feiyan Zhao, Zhihong Sun
{"title":"分娩方式和母体妊娠糖尿病是影响新生儿初始肠道微生物群的重要因素","authors":"Xuan Shi, Yanfang Liu, Teng Ma, Hao Jin, Feiyan Zhao, Zhihong Sun","doi":"10.3389/fcimb.2024.1397675","DOIUrl":null,"url":null,"abstract":"BackgroundThe infant gut microbiome’s establishment is pivotal for health and immune development. Understanding it unveils insights into growth, development, and maternal microbial interactions. Research often emphasizes gut bacteria, neglecting the phageome.MethodsTo investigate the influence of geographic or maternal factors (mode of delivery, mode of breastfeeding, gestational diabetes mellitus) on the gut microbiota and phages of newborns, we collected fecal samples from 34 pairs of mothers and their infants within 24 hours of delivery from three regions (9 pairs from Enshi, 7 pairs from Hohhot, and 18 pairs from Hulunbuir) using sterile containers. Gut microbiota analysis by Shotgun sequencing was subsequently performed.ResultsOur results showed that geographic location affects maternal gut microbiology (<jats:italic>P</jats:italic> &lt; 0.05), while the effect on infant gut microbiology was not significant (<jats:italic>P</jats:italic> = 0.184). Among the maternal factors, mode of delivery had a significant (<jats:italic>P</jats:italic> &lt; 0.05) effect on the newborn. Specific bacteria (e.g., <jats:italic>Bacteroides</jats:italic>, <jats:italic>Escherichia</jats:italic> spp., <jats:italic>Phocaeicola vulgatus</jats:italic>, <jats:italic>Escherichia coli</jats:italic>, <jats:italic>Staphylococcus hominis</jats:italic>, <jats:italic>Veillonella</jats:italic> spp.), predicted active metabolites, and bacteriophage vOTUs varied with delivery mode. <jats:italic>Phocaeicola vulgatus</jats:italic> significantly correlated with some metabolites and bacteriophages in the early infant gut (<jats:italic>P</jats:italic> &lt; 0.05). In the GD group, a strong negative correlation of phage diversity between mother and infants was observed (<jats:italic>R</jats:italic> = -0.58, <jats:italic>P</jats:italic>=0.04).ConclusionIn conclusion, neonatal early gut microbiome (including bacteria and bacteriophages) colonization is profoundly affected by the mode of delivery, and maternal gestational diabetes mellitus. The key bacteria may interact with bacteriophages to influence the levels of specific metabolites. Our study provides new evidence for the study of the infant microbiome, fills a gap in the analysis of the infant gut microbiota regarding the virome, and emphasizes the importance of maternal health for the infant initial gut virome.","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Delivery mode and maternal gestational diabetes are important factors in shaping the neonatal initial gut microbiota\",\"authors\":\"Xuan Shi, Yanfang Liu, Teng Ma, Hao Jin, Feiyan Zhao, Zhihong Sun\",\"doi\":\"10.3389/fcimb.2024.1397675\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"BackgroundThe infant gut microbiome’s establishment is pivotal for health and immune development. Understanding it unveils insights into growth, development, and maternal microbial interactions. Research often emphasizes gut bacteria, neglecting the phageome.MethodsTo investigate the influence of geographic or maternal factors (mode of delivery, mode of breastfeeding, gestational diabetes mellitus) on the gut microbiota and phages of newborns, we collected fecal samples from 34 pairs of mothers and their infants within 24 hours of delivery from three regions (9 pairs from Enshi, 7 pairs from Hohhot, and 18 pairs from Hulunbuir) using sterile containers. Gut microbiota analysis by Shotgun sequencing was subsequently performed.ResultsOur results showed that geographic location affects maternal gut microbiology (<jats:italic>P</jats:italic> &lt; 0.05), while the effect on infant gut microbiology was not significant (<jats:italic>P</jats:italic> = 0.184). Among the maternal factors, mode of delivery had a significant (<jats:italic>P</jats:italic> &lt; 0.05) effect on the newborn. Specific bacteria (e.g., <jats:italic>Bacteroides</jats:italic>, <jats:italic>Escherichia</jats:italic> spp., <jats:italic>Phocaeicola vulgatus</jats:italic>, <jats:italic>Escherichia coli</jats:italic>, <jats:italic>Staphylococcus hominis</jats:italic>, <jats:italic>Veillonella</jats:italic> spp.), predicted active metabolites, and bacteriophage vOTUs varied with delivery mode. <jats:italic>Phocaeicola vulgatus</jats:italic> significantly correlated with some metabolites and bacteriophages in the early infant gut (<jats:italic>P</jats:italic> &lt; 0.05). In the GD group, a strong negative correlation of phage diversity between mother and infants was observed (<jats:italic>R</jats:italic> = -0.58, <jats:italic>P</jats:italic>=0.04).ConclusionIn conclusion, neonatal early gut microbiome (including bacteria and bacteriophages) colonization is profoundly affected by the mode of delivery, and maternal gestational diabetes mellitus. The key bacteria may interact with bacteriophages to influence the levels of specific metabolites. Our study provides new evidence for the study of the infant microbiome, fills a gap in the analysis of the infant gut microbiota regarding the virome, and emphasizes the importance of maternal health for the infant initial gut virome.\",\"PeriodicalId\":12458,\"journal\":{\"name\":\"Frontiers in Cellular and Infection Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-08-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Cellular and Infection Microbiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3389/fcimb.2024.1397675\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Cellular and Infection Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/fcimb.2024.1397675","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Delivery mode and maternal gestational diabetes are important factors in shaping the neonatal initial gut microbiota
BackgroundThe infant gut microbiome’s establishment is pivotal for health and immune development. Understanding it unveils insights into growth, development, and maternal microbial interactions. Research often emphasizes gut bacteria, neglecting the phageome.MethodsTo investigate the influence of geographic or maternal factors (mode of delivery, mode of breastfeeding, gestational diabetes mellitus) on the gut microbiota and phages of newborns, we collected fecal samples from 34 pairs of mothers and their infants within 24 hours of delivery from three regions (9 pairs from Enshi, 7 pairs from Hohhot, and 18 pairs from Hulunbuir) using sterile containers. Gut microbiota analysis by Shotgun sequencing was subsequently performed.ResultsOur results showed that geographic location affects maternal gut microbiology (P < 0.05), while the effect on infant gut microbiology was not significant (P = 0.184). Among the maternal factors, mode of delivery had a significant (P < 0.05) effect on the newborn. Specific bacteria (e.g., Bacteroides, Escherichia spp., Phocaeicola vulgatus, Escherichia coli, Staphylococcus hominis, Veillonella spp.), predicted active metabolites, and bacteriophage vOTUs varied with delivery mode. Phocaeicola vulgatus significantly correlated with some metabolites and bacteriophages in the early infant gut (P < 0.05). In the GD group, a strong negative correlation of phage diversity between mother and infants was observed (R = -0.58, P=0.04).ConclusionIn conclusion, neonatal early gut microbiome (including bacteria and bacteriophages) colonization is profoundly affected by the mode of delivery, and maternal gestational diabetes mellitus. The key bacteria may interact with bacteriophages to influence the levels of specific metabolites. Our study provides new evidence for the study of the infant microbiome, fills a gap in the analysis of the infant gut microbiota regarding the virome, and emphasizes the importance of maternal health for the infant initial gut virome.
期刊介绍:
Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.