多尺度水凝胶调节间充质干细胞命运,促进骨再生

IF 7.9 2区 综合性期刊 Q1 CHEMISTRY, MULTIDISCIPLINARY
Ze Li, Jiayang Li, Guiwen Qu, Kang Chen, Ye Liu, Sicheng Li, Canwen Chen, Yun Zhao, Jinjian Huang, Peige Wang, Xiuwen Wu, Jianan Ren
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引用次数: 0

摘要

水凝胶通常被用作三维细胞培养平台。高硬度水凝胶可促进细胞定向分化,但也可能限制细胞活性。在此,我们报告了一种利用牺牲模板和纳米粒子制备多尺度水凝胶的方法,这种水凝胶具有大孔和局部增强的硬度特性。大孔水凝胶为细胞提供了充足的空间,有利于细胞的活动和增殖。纳米粒子的化学掺杂可在不影响其宏观机械性能的情况下产生局部刚度增强区域。这种区域硬度可促进包裹的脂肪间充质干细胞(ADSCs)的成骨分化。重要的是,在水凝胶中进行成骨分化后,ADSCs 的功能活性显著增强。值得注意的是,水凝胶能有效激活 ADSCs 的机械传导信号并影响其命运。此外,在动物实验中,ADSC负载的多尺度水凝胶促进了大鼠颅骨缺损的骨再生。总之,我们的研究结果表明,这项技术在生物医学领域有着广阔的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Multiscale hydrogel regulates mesenchymal stem cell fate for bone regeneration

Multiscale hydrogel regulates mesenchymal stem cell fate for bone regeneration

Hydrogels are commonly utilized as a three-dimensional cell culture platform. High-stiffness hydrogels promote directional cell differentiation, but they may also restrict cellular activity. Here, we report a process utilizing sacrificial templates and nanoparticles for the preparation of multiscale hydrogels with macroporous and locally enhanced stiffness properties. The macroporous hydrogels provide ample space for cells, which facilitates cell activity and proliferation. Chemical doping of the nanoparticles creates a locally stiffness-enhanced region without affecting its macroscopic mechanical properties. This regional stiffness promotes osteogenic differentiation of encapsulated adipose-derived mesenchymal stem cells (ADSCs). Importantly, the functional activity of the ADSCs increases significantly after osteogenic differentiation in hydrogels. Notably, the hydrogels efficiently activate mechanotransduction signals in the ADSCs and influence their fate. In addition, ADSC-loaded multiscale hydrogels promote bone regeneration of rat cranial defects in animal experiments. Collectively, our findings demonstrate that this technique has promising applications in the biomedical field.

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来源期刊
Cell Reports Physical Science
Cell Reports Physical Science Energy-Energy (all)
CiteScore
11.40
自引率
2.20%
发文量
388
审稿时长
62 days
期刊介绍: Cell Reports Physical Science, a premium open-access journal from Cell Press, features high-quality, cutting-edge research spanning the physical sciences. It serves as an open forum fostering collaboration among physical scientists while championing open science principles. Published works must signify significant advancements in fundamental insight or technological applications within fields such as chemistry, physics, materials science, energy science, engineering, and related interdisciplinary studies. In addition to longer articles, the journal considers impactful short-form reports and short reviews covering recent literature in emerging fields. Continually adapting to the evolving open science landscape, the journal reviews its policies to align with community consensus and best practices.
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