基于萘吡喃的光开关,利用相敏模式实现更简单的化学传感和成像

IF 7.9 2区 综合性期刊 Q1 CHEMISTRY, MULTIDISCIPLINARY
Yu Cheng, Yifu Wang, Jingying Zhai, Xiaojiang Xie
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引用次数: 0

摘要

基于强度的荧光成像受到光谱重叠和光学背景干扰的影响。作为替代方法,纳秒级的荧光寿命测量在很大程度上也受到限制。在此,我们提出了对含有萘并吡喃和荧光供体的光开关探针进行相敏检测的方法。该方法的特点是反应动力学在毫秒到秒的范围内,可使用经济高效的设备进行频域检测。探针荧光中的相移(Δj)是通过快速傅立叶变换提取的,它与供体和受体的摩尔比有关。因此,Δj 被认为是选择性突出荧光探针的自参照量,也是化学传感中的动态信号读数。基于该平台,成功实现了对原胺的相敏检测,原胺是一种多阳离子蛋白质,在手术中常被用作抗凝剂肝素的中和剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Naphthopyran-based photoswitching for simpler chemical sensing and imaging using phase-sensitive mode

Naphthopyran-based photoswitching for simpler chemical sensing and imaging using phase-sensitive mode

Intensity-based fluorescence imaging suffers from spectral overlap and optical background interference. As an alternative, fluorescence lifetime measurements on the nanosecond level are also largely constrained. Herein, we propose phase-sensitive detection of photoswitchable probes containing naphthopyran and fluorescent donors. The method features reaction kinetics in the millisecond-to-second regime, allowing frequency domain detection with cost-effective equipment. A phase shift (Δϕ) in the fluorescence of the probes is extracted by fast Fourier transform, establishing a dependence on the molar ratio of donor to acceptor. Thus, Δϕ is proposed as a self-referencing quantity for selective highlighting of fluorescent probes and a dynamic signal readout in chemical sensing. Phase-sensitive detection of protamine, a polycationic protein often used as a neutralizer of the anticoagulant heparin during surgery, is successfully realized based on the platform.

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来源期刊
Cell Reports Physical Science
Cell Reports Physical Science Energy-Energy (all)
CiteScore
11.40
自引率
2.20%
发文量
388
审稿时长
62 days
期刊介绍: Cell Reports Physical Science, a premium open-access journal from Cell Press, features high-quality, cutting-edge research spanning the physical sciences. It serves as an open forum fostering collaboration among physical scientists while championing open science principles. Published works must signify significant advancements in fundamental insight or technological applications within fields such as chemistry, physics, materials science, energy science, engineering, and related interdisciplinary studies. In addition to longer articles, the journal considers impactful short-form reports and short reviews covering recent literature in emerging fields. Continually adapting to the evolving open science landscape, the journal reviews its policies to align with community consensus and best practices.
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