Ivana Regina da Costa, Rafaela Pires Erthal, Suellen Ribeiro da Silva Scarton, Suellen Miguez Gonzalez, Marcela Bortoletto Cerezetti, Fábio Morotti, Marcelo Marcondes Seneda, Glaura Scantamburlo Alves Fernandes
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引用次数: 0
摘要
监管部门和科学界对能够干扰生殖系统的环境物质的研究兴趣日益浓厚。其中,对羟基苯甲酸酯类物质因其广泛的使用和在人体组织和生物液体中的频繁检测而脱颖而出。因此,我们评估了对羟基苯甲酸丁酯对体外牛卵泡活力和卵泡分期的毒性影响。五头周期性雌牛的卵巢片段在最基本培养基(MEM;对照组)或添加 50 µg/mL 和 100 µg/mL 对羟基苯甲酸丁酯的 MEM(分别为 BP 50 组和 BP 100 组)中培养 44 小时。对卵巢碎片进行卵泡分期、形态分析、形态计量分析、雌二醇分析和氧化谱分析。在卵泡分期、雌二醇分析和氧化谱分析方面,各实验组之间没有观察到明显的变化。然而,BP 50 组的完整卵泡数量明显减少。此外,在暴露于苯甲酸丁酯的各组中还观察到卵泡和卵母细胞直径的下降。总之,在体外牛模型中,丁基苯甲酸酯会损害卵巢卵泡的完整性和大小,但不会影响氧化概况和类固醇生成。
In vitro exposure to butylparaben impairs the integrity and size of ovarian follicles in a bovine model
There is a growing regulatory and scientific interest in the studies of environmental substances that are capable of interfering with the reproductive system. Among them, parabens stand out due to their widespread use and frequent detection as contaminants in human tissues and biological fluids. Therefore, we evaluated the toxic effects of butylparaben on the viability and follicular staging of bovine ovarian follicles in vitro. Fragments of ovaries from five cyclic bovine females were cultured for 44 h in a minimal essential medium (MEM; control) or MEM supplemented with 50 µg/mL and 100 µg/mL of butylparaben (BP 50 and BP 100 groups, respectively). The ovarian fragments were subjected to follicular staging, morphological analysis, morphometric analysis, estradiol analysis and oxidative profiling. No significant changes were observed between the experimental groups in follicular staging, estradiol analysis and oxidative profile analysis. However, the BP 50 group showed a significant decrease in the number of intact ovarian follicles. Moreover, a decrease in the follicular and oocyte diameters was observed in the groups that were exposed to butylparaben. In conclusion, butylparaben impairs the integrity and size of ovarian follicles in an in vitro bovine model, but does not affect the oxidative profile and steroidogenesis.
期刊介绍:
Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial.
The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.