亲水性金属有机框架诱导碱性磷酸酶的界面活化

IF 9.5 2区 材料科学 Q1 CHEMISTRY, PHYSICAL
Dongyan Chen, Yi Xu, Jie Wei, Munetaka Oyama, Quansheng Chen, Xiaomei Chen
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引用次数: 0

摘要

将天然酶封装在金属有机框架(MOFs)中,可以在恶劣环境中保持酶原有的生物功能。然而,目前尚不清楚 MOF 与酶之间界面相互作用的性质,因此难以有效调节酶@MOF 复合材料的生物催化活性。MOF 载体亲水性的差异与酶的构象变化和催化特性密切相关。本研究利用实验方法和分子动力学模拟系统研究了亲水性沸石咪唑酸盐框架-90(ZIF-90)和疏水性 ZIF-8 封装的碱性磷酸酶(ALP)的催化活性、稳定性和构象变化。结果表明,亲水性 ZIF-90 包封的 ALP 具有更高的稳定性,在使用 20 个周期后,其催化活性的保持率是疏水性 ALP@ZIF-8 的 2.22 倍。此外,ZIF-90 提供的亲水界面有效调节了 ALP 的结构,使其活性中心保持最佳催化构象。通过将高生物活性 ALP@ZIF-90 应用于自校准比色/荧光双模式传感方法,对甲基对硫磷进行高效、可靠和准确的检测,证明了它的实际应用价值。这项研究为改进酶固定化策略和促进酶@MOF 复合材料在催化和传感应用领域的快速发展提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Interfacial activation of alkaline phosphatase induced by hydrophilic metal—organic frameworks

Interfacial activation of alkaline phosphatase induced by hydrophilic metal—organic frameworks

Encapsulating natural enzymes in metal—organic frameworks (MOFs) can maintain the original biological functions of enzymes in harsh environments. However, the nature of interfacial interactions between a MOF and enzyme is currently unclear, rendering effective regulation of the biocatalytic activity of the enzyme@MOF composite difficult. Differences in the hydrophilicity of MOF carriers are closely related to the conformational changes and catalytic properties of the enzyme. In this study, the catalytic activity, stability, and conformational changes of alkaline phosphatase (ALP) encapsulated in hydrophilic zeolite imidazolate framework-90 (ZIF-90) and hydrophobic ZIF-8 were systematically investigated using experimental methods and molecular dynamics simulations. The results demonstrated that hydrophilic ZIF-90-encapsulated ALP exhibited superior stability and was 2.22-fold more retained catalytically active than hydrophobic ALP@ZIF-8 after 20 cycles of utilization. Moreover, the hydrophilic interface provided by ZIF-90 effectively regulated the structure of ALP to maintain the optimal catalytic conformation of its active center. The practical application of highly bioactive ALP@ZIF-90 was demonstrated by employing it in a self-calibrated colorimetric/fluorescence dual-mode sensing method for the efficient, reliable, and accurate detection of methyl paraoxon. This study provides new insights for improving enzyme immobilization strategies and promoting the rapid development of enzyme@MOF composites for catalytic and sensing applications.

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来源期刊
Nano Research
Nano Research 化学-材料科学:综合
CiteScore
14.30
自引率
11.10%
发文量
2574
审稿时长
1.7 months
期刊介绍: Nano Research is a peer-reviewed, international and interdisciplinary research journal that focuses on all aspects of nanoscience and nanotechnology. It solicits submissions in various topical areas, from basic aspects of nanoscale materials to practical applications. The journal publishes articles on synthesis, characterization, and manipulation of nanomaterials; nanoscale physics, electrical transport, and quantum physics; scanning probe microscopy and spectroscopy; nanofluidics; nanosensors; nanoelectronics and molecular electronics; nano-optics, nano-optoelectronics, and nano-photonics; nanomagnetics; nanobiotechnology and nanomedicine; and nanoscale modeling and simulations. Nano Research offers readers a combination of authoritative and comprehensive Reviews, original cutting-edge research in Communication and Full Paper formats. The journal also prioritizes rapid review to ensure prompt publication.
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