Chia-Yi Cheng, Eldhose V. Varghese, Wen-Jyun Wang, Chia-Yu Yao, Chia-Hsiang Chen, Wei-Peng Li
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Aggregation-induced emission silence-mediated pathogen detection using a rapidly degradable nanographene-embedded polymersome
Typical pathogen detection processes are time-consuming and require expensive equipment and professional operators, limiting their practical applicability. Developing a rapid and easy-to-read method of accurately sensing pathogenic bacteria is critical for reducing the spread and risk of infection in high-risk areas. Herein, the synthesis of nanographene (nanoG) that exhibits aggregation-induced emission (AIE) is described. The nanoG was embedded into a hydrophobic shell of poly(lactic-co-glycolic acid) (PLGA) polymersome in a double-emulsion process, significantly enhancing the nanoG luminescence under irradiation at 330 nm due to the enrichment of nanoG between the inner and outer PLGA shells. Both Gram-positive and Gram-negative bacteria can rapidly degrade the PLGA vesicular structure, leading to dispersal of the nanoG inside the shell and silencing the AIE effect. A linear relationship between the bacterial concentration and emissivity was established, and the detection limit was identified. Moreover, the polymersome has excellent selectivity for methicillin-resistant Staphylococcus aureus (MRSA) detection after a screening pretreatment of a bacterial mixture with suitable antibiotics. The AIE silencing could be observed with the naked eye in an MRSA-infected wound treated with the polymersome after 1 h of incubation, demonstrating a high potential for clinical rapid screening applications.
期刊介绍:
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive:
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices