Mohammad Reza Salmani, Fazelehsadat Shirazi, Kasra Goodarzi, Fatemeh Noormohammadi, Mohammad Nourany
{"title":"利用 CNC/PCL-PEG-PCL 三嵌段共聚物改善聚乳酸-聚CL 基质-液滴混合物的相容性以制备多孔三维骨诱导支架","authors":"Mohammad Reza Salmani, Fazelehsadat Shirazi, Kasra Goodarzi, Fatemeh Noormohammadi, Mohammad Nourany","doi":"10.1007/s10924-024-03392-5","DOIUrl":null,"url":null,"abstract":"<p>Attempts were made to produce osteoinductive three- dimensional (3D) porous scaffolds with interconnected pores. Among the synthetic polymers, poly (ε- caprolactone) (PCL) was proven to be highly biocompatible and osteoinductive. However, it suffers from shrinkage due to high crystallinity and fast crystal growth. Here, we used polylactic acid, which is a rigid, low- crystalline and biocompatible polymer, as the major phase blended with PCL comprising the minor phase. However, these two polymers are highly incompatible and the PCL minor phase tend to form large droplets, distributing it unevenly throughout the continuous phase. Here, we used cellulose nanocrystals (CNCs), as a hydrophilic and osteoinductive nanoparticle, to suppress coalescence of PCL droplets and a tri-block copolymer of PCL-PEG-PCL (BCP) to reduce the interfacial tension of the two phases. 3D foams were prepared using thermally-induced phase separation and salt leaching and the porosity and pore size was tuned using CNC and BCP. The biocompatibility of the 3D scaffolds was evaluated by MG63 cell lines and the results indicated high biocompatibility. The scaffolds were also quite successful at inducing the osteogenesis of hMSCs. The specimen containing 10% BCP and 1.0% CNC had the highest calcium deposition with the highest expression of bone- specific genes.</p>","PeriodicalId":659,"journal":{"name":"Journal of Polymers and the Environment","volume":null,"pages":null},"PeriodicalIF":4.7000,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Improving Phase Compatibility of PLA-PCL Matrix-Droplet Blend Using CNC/PCL-PEG-PCL Triblock Copolymer to Prepare Porous 3D Osteoinductive Scaffolds\",\"authors\":\"Mohammad Reza Salmani, Fazelehsadat Shirazi, Kasra Goodarzi, Fatemeh Noormohammadi, Mohammad Nourany\",\"doi\":\"10.1007/s10924-024-03392-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Attempts were made to produce osteoinductive three- dimensional (3D) porous scaffolds with interconnected pores. Among the synthetic polymers, poly (ε- caprolactone) (PCL) was proven to be highly biocompatible and osteoinductive. However, it suffers from shrinkage due to high crystallinity and fast crystal growth. Here, we used polylactic acid, which is a rigid, low- crystalline and biocompatible polymer, as the major phase blended with PCL comprising the minor phase. However, these two polymers are highly incompatible and the PCL minor phase tend to form large droplets, distributing it unevenly throughout the continuous phase. Here, we used cellulose nanocrystals (CNCs), as a hydrophilic and osteoinductive nanoparticle, to suppress coalescence of PCL droplets and a tri-block copolymer of PCL-PEG-PCL (BCP) to reduce the interfacial tension of the two phases. 3D foams were prepared using thermally-induced phase separation and salt leaching and the porosity and pore size was tuned using CNC and BCP. The biocompatibility of the 3D scaffolds was evaluated by MG63 cell lines and the results indicated high biocompatibility. The scaffolds were also quite successful at inducing the osteogenesis of hMSCs. The specimen containing 10% BCP and 1.0% CNC had the highest calcium deposition with the highest expression of bone- specific genes.</p>\",\"PeriodicalId\":659,\"journal\":{\"name\":\"Journal of Polymers and the Environment\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2024-09-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Polymers and the Environment\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s10924-024-03392-5\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Polymers and the Environment","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s10924-024-03392-5","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Improving Phase Compatibility of PLA-PCL Matrix-Droplet Blend Using CNC/PCL-PEG-PCL Triblock Copolymer to Prepare Porous 3D Osteoinductive Scaffolds
Attempts were made to produce osteoinductive three- dimensional (3D) porous scaffolds with interconnected pores. Among the synthetic polymers, poly (ε- caprolactone) (PCL) was proven to be highly biocompatible and osteoinductive. However, it suffers from shrinkage due to high crystallinity and fast crystal growth. Here, we used polylactic acid, which is a rigid, low- crystalline and biocompatible polymer, as the major phase blended with PCL comprising the minor phase. However, these two polymers are highly incompatible and the PCL minor phase tend to form large droplets, distributing it unevenly throughout the continuous phase. Here, we used cellulose nanocrystals (CNCs), as a hydrophilic and osteoinductive nanoparticle, to suppress coalescence of PCL droplets and a tri-block copolymer of PCL-PEG-PCL (BCP) to reduce the interfacial tension of the two phases. 3D foams were prepared using thermally-induced phase separation and salt leaching and the porosity and pore size was tuned using CNC and BCP. The biocompatibility of the 3D scaffolds was evaluated by MG63 cell lines and the results indicated high biocompatibility. The scaffolds were also quite successful at inducing the osteogenesis of hMSCs. The specimen containing 10% BCP and 1.0% CNC had the highest calcium deposition with the highest expression of bone- specific genes.
期刊介绍:
The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.