利用 CNC/PCL-PEG-PCL 三嵌段共聚物改善聚乳酸-聚CL 基质-液滴混合物的相容性以制备多孔三维骨诱导支架

IF 4.7 3区 工程技术 Q2 ENGINEERING, ENVIRONMENTAL
Mohammad Reza Salmani, Fazelehsadat Shirazi, Kasra Goodarzi, Fatemeh Noormohammadi, Mohammad Nourany
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引用次数: 0

摘要

人们尝试制造具有相互连接孔隙的诱导骨生成三维(3D)多孔支架。在合成聚合物中,聚(ε-己内酯)(PCL)被证明具有很高的生物相容性和骨诱导性。然而,由于结晶度高、晶体生长快,聚乳酸存在收缩问题。在这里,我们使用聚乳酸(一种刚性、低结晶度和生物相容性高的聚合物)作为主要相,PCL 作为次要相进行混合。然而,这两种聚合物极不相容,而且 PCL 小相容易形成大液滴,在整个连续相中分布不均。在此,我们使用纤维素纳米晶体(CNCs)作为亲水性和骨诱导性纳米粒子来抑制 PCL 液滴的凝聚,并使用 PCL-PEG-PCL 三嵌段共聚物(BCP)来降低两相的界面张力。利用热诱导相分离和盐浸法制备了三维泡沫,并使用 CNC 和 BCP 调整了孔隙率和孔径。MG63 细胞系对三维支架的生物相容性进行了评估,结果表明支架具有很高的生物相容性。支架在诱导 hMSCs 成骨方面也相当成功。含 10% BCP 和 1.0% CNC 的试样钙沉积最高,骨特异性基因的表达也最高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Improving Phase Compatibility of PLA-PCL Matrix-Droplet Blend Using CNC/PCL-PEG-PCL Triblock Copolymer to Prepare Porous 3D Osteoinductive Scaffolds

Improving Phase Compatibility of PLA-PCL Matrix-Droplet Blend Using CNC/PCL-PEG-PCL Triblock Copolymer to Prepare Porous 3D Osteoinductive Scaffolds

Attempts were made to produce osteoinductive three- dimensional (3D) porous scaffolds with interconnected pores. Among the synthetic polymers, poly (ε- caprolactone) (PCL) was proven to be highly biocompatible and osteoinductive. However, it suffers from shrinkage due to high crystallinity and fast crystal growth. Here, we used polylactic acid, which is a rigid, low- crystalline and biocompatible polymer, as the major phase blended with PCL comprising the minor phase. However, these two polymers are highly incompatible and the PCL minor phase tend to form large droplets, distributing it unevenly throughout the continuous phase. Here, we used cellulose nanocrystals (CNCs), as a hydrophilic and osteoinductive nanoparticle, to suppress coalescence of PCL droplets and a tri-block copolymer of PCL-PEG-PCL (BCP) to reduce the interfacial tension of the two phases. 3D foams were prepared using thermally-induced phase separation and salt leaching and the porosity and pore size was tuned using CNC and BCP. The biocompatibility of the 3D scaffolds was evaluated by MG63 cell lines and the results indicated high biocompatibility. The scaffolds were also quite successful at inducing the osteogenesis of hMSCs. The specimen containing 10% BCP and 1.0% CNC had the highest calcium deposition with the highest expression of bone- specific genes.

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来源期刊
Journal of Polymers and the Environment
Journal of Polymers and the Environment 工程技术-高分子科学
CiteScore
9.50
自引率
7.50%
发文量
297
审稿时长
9 months
期刊介绍: The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.
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