{"title":"用于灵敏检测黄曲霉毒素 B1 的光热和表面增强拉曼散射双模式免疫层析传感器","authors":"Qian Wang, Yongjiao Ren, Shijie Li, Junping Wang","doi":"10.1016/j.microc.2024.111604","DOIUrl":null,"url":null,"abstract":"Multimodal lateral flow immunoassay has displayed the great potential to improve the flexibility and practicality of point-of-care testing. Herein, this study developed a dual-mode photothermal (PT) and surface-enhanced Raman scattering (SERS) immunochromatographic sensor for sensitive detection of aflatoxin B (AFB). The bifunctional waxberry-like core-satellite nanoparticles loaded with 5,5′-Dithiobis (2-nitrobenzoic acid) (DTNB) were prepared and coupled with antibody to form PT@SERS nanoprobes for qualitative and quantitative detection of AFB. The photothermal conversion efficiency and SERS enhancement factor of the nanoprobes were 42.11 % and 1.59 × 10, respectively. Under the optimal conditions, the limit of detection of PT assay was 0.033 ng/mL with a linear range of 0.05–10 ng/mL (R = 0.997); the limit of detection of SERS assay was 0.0073 ng/mL with a linear range of 0.005–10 ng/mL (R = 0.998). The results of the specificity analysis indicated no cross-reactions with the other toxins. The recoveries of the spiked corn and peanut were from 85.39 % to 112.15 % (PT assay) and 80.04 % to 106.57 % (SERS assay), respectively. The assay demonstrated that the developed dual-mode sensor provided a promising option for achieving the rapid detection of AFB.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.9000,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A dual-mode immunochromatographic sensor with photothermal and surface-enhanced Raman scattering for sensitive detection of aflatoxin B1\",\"authors\":\"Qian Wang, Yongjiao Ren, Shijie Li, Junping Wang\",\"doi\":\"10.1016/j.microc.2024.111604\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Multimodal lateral flow immunoassay has displayed the great potential to improve the flexibility and practicality of point-of-care testing. Herein, this study developed a dual-mode photothermal (PT) and surface-enhanced Raman scattering (SERS) immunochromatographic sensor for sensitive detection of aflatoxin B (AFB). The bifunctional waxberry-like core-satellite nanoparticles loaded with 5,5′-Dithiobis (2-nitrobenzoic acid) (DTNB) were prepared and coupled with antibody to form PT@SERS nanoprobes for qualitative and quantitative detection of AFB. The photothermal conversion efficiency and SERS enhancement factor of the nanoprobes were 42.11 % and 1.59 × 10, respectively. Under the optimal conditions, the limit of detection of PT assay was 0.033 ng/mL with a linear range of 0.05–10 ng/mL (R = 0.997); the limit of detection of SERS assay was 0.0073 ng/mL with a linear range of 0.005–10 ng/mL (R = 0.998). The results of the specificity analysis indicated no cross-reactions with the other toxins. The recoveries of the spiked corn and peanut were from 85.39 % to 112.15 % (PT assay) and 80.04 % to 106.57 % (SERS assay), respectively. The assay demonstrated that the developed dual-mode sensor provided a promising option for achieving the rapid detection of AFB.\",\"PeriodicalId\":391,\"journal\":{\"name\":\"Microchemical Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.9000,\"publicationDate\":\"2024-09-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchemical Journal\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.microc.2024.111604\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchemical Journal","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.microc.2024.111604","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
A dual-mode immunochromatographic sensor with photothermal and surface-enhanced Raman scattering for sensitive detection of aflatoxin B1
Multimodal lateral flow immunoassay has displayed the great potential to improve the flexibility and practicality of point-of-care testing. Herein, this study developed a dual-mode photothermal (PT) and surface-enhanced Raman scattering (SERS) immunochromatographic sensor for sensitive detection of aflatoxin B (AFB). The bifunctional waxberry-like core-satellite nanoparticles loaded with 5,5′-Dithiobis (2-nitrobenzoic acid) (DTNB) were prepared and coupled with antibody to form PT@SERS nanoprobes for qualitative and quantitative detection of AFB. The photothermal conversion efficiency and SERS enhancement factor of the nanoprobes were 42.11 % and 1.59 × 10, respectively. Under the optimal conditions, the limit of detection of PT assay was 0.033 ng/mL with a linear range of 0.05–10 ng/mL (R = 0.997); the limit of detection of SERS assay was 0.0073 ng/mL with a linear range of 0.005–10 ng/mL (R = 0.998). The results of the specificity analysis indicated no cross-reactions with the other toxins. The recoveries of the spiked corn and peanut were from 85.39 % to 112.15 % (PT assay) and 80.04 % to 106.57 % (SERS assay), respectively. The assay demonstrated that the developed dual-mode sensor provided a promising option for achieving the rapid detection of AFB.
期刊介绍:
The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field.
Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.