Xiaoqing Hu, Tong Zheng, Wenjie Chen, Huilei Duan, Zhongjia Yuan, Jiaqian An, Huihui Zhang, Xuemei Liu
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GST family genes showed collinearity with 22 genes in <i>Oryza sativa</i> L., and three genes in <i>Arabidopsis thaliana</i>; promoter <i>cis</i>-acting elements predicted that the GST gene family is functional in growth, hormone regulation, and abiotic stress response. Most members of the F subfamily of <i>GST</i> (<i>BpGSTFs</i>) were expressed in roots, stems, leaves, and petioles, with the most expression observed in leaves. On the basis of the expression profiles of F subfamily genes (<i>BpGSTF1</i> to <i>BpGSTF13</i>) during salt, mannitol and ABA stress, <i>BpGSTF</i> proteins seem to have multiple functions depending on the type of abiotic stress; for instance, <i>BpGSTs</i> may function at different times during abiotic stress. 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In this study, 71 members of the <i>BpGST</i> family were identified from the entire <i>Betula platyphylla</i> Suk. genome. Most of the members encode proteins with amino acid lengths ranging from 101 to 875 and were localized to the cytoplasm by a prediction. <i>BpGSTs</i> can be divided into seven subfamilies, with a majority of birch U and F subfamily members according to gene structure, conserved motifs and evolutionary analysis. GST family genes showed collinearity with 22 genes in <i>Oryza sativa</i> L., and three genes in <i>Arabidopsis thaliana</i>; promoter <i>cis</i>-acting elements predicted that the GST gene family is functional in growth, hormone regulation, and abiotic stress response. Most members of the F subfamily of <i>GST</i> (<i>BpGSTFs</i>) were expressed in roots, stems, leaves, and petioles, with the most expression observed in leaves. On the basis of the expression profiles of F subfamily genes (<i>BpGSTF1</i> to <i>BpGSTF13</i>) during salt, mannitol and ABA stress, <i>BpGSTF</i> proteins seem to have multiple functions depending on the type of abiotic stress; for instance, <i>BpGSTs</i> may function at different times during abiotic stress. 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引用次数: 0
摘要
谷胱甘肽-S-转移酶(GST,EC2.5.1.18)是一种多功能蛋白酶,对植物的解毒、抵御生物和非生物胁迫以及植物生长发育过程中的次生代谢物质运输具有重要作用。本研究从桦树(Betula platyphylla Suk.)的整个基因组中鉴定出 71 个 BpGST 家族成员。大多数成员编码的蛋白质氨基酸长度在 101 至 875 之间,并通过预测被定位到细胞质中。根据基因结构、保守基序和进化分析,BpGST 可分为七个亚家族,其中桦木 U 和 F 亚家族成员居多。GST家族基因与拟南芥(Oryza sativa L.)中的22个基因和拟南芥(Arabidopsis thaliana)中的3个基因具有共线性;启动子顺式作用元件预测GST基因家族在生长、激素调节和非生物胁迫响应方面具有功能。GST F 亚家族(BpGSTFs)的大多数成员在根、茎、叶和叶柄中表达,其中在叶片中的表达量最大。根据F亚家族基因(BpGSTF1至BpGSTF13)在盐胁迫、甘露醇胁迫和ABA胁迫中的表达谱,BpGSTF蛋白似乎具有多种功能,这取决于非生物胁迫的类型;例如,BpGSTs可能在非生物胁迫的不同时期发挥作用。这项研究加深了人们对 GST 基因家族的了解,为进一步探索它们在桦树中的功能奠定了基础。
Genome-wide identification and expression analysis of the GST gene family of Betula platyphylla
Glutathione-S-transferase (GST, EC2.5.1.18) multifunctional protease is important for detoxification, defense against biotic and abiotic stresses, and secondary metabolic material transport for plant growth and development. In this study, 71 members of the BpGST family were identified from the entire Betula platyphylla Suk. genome. Most of the members encode proteins with amino acid lengths ranging from 101 to 875 and were localized to the cytoplasm by a prediction. BpGSTs can be divided into seven subfamilies, with a majority of birch U and F subfamily members according to gene structure, conserved motifs and evolutionary analysis. GST family genes showed collinearity with 22 genes in Oryza sativa L., and three genes in Arabidopsis thaliana; promoter cis-acting elements predicted that the GST gene family is functional in growth, hormone regulation, and abiotic stress response. Most members of the F subfamily of GST (BpGSTFs) were expressed in roots, stems, leaves, and petioles, with the most expression observed in leaves. On the basis of the expression profiles of F subfamily genes (BpGSTF1 to BpGSTF13) during salt, mannitol and ABA stress, BpGSTF proteins seem to have multiple functions depending on the type of abiotic stress; for instance, BpGSTs may function at different times during abiotic stress. This study enhances understanding of the GST gene family and provides a basis for further exploration of their function in birch.
期刊介绍:
The Journal of Forestry Research (JFR), founded in 1990, is a peer-reviewed quarterly journal in English. JFR has rapidly emerged as an international journal published by Northeast Forestry University and Ecological Society of China in collaboration with Springer Verlag. The journal publishes scientific articles related to forestry for a broad range of international scientists, forest managers and practitioners.The scope of the journal covers the following five thematic categories and 20 subjects:
Basic Science of Forestry,
Forest biometrics,
Forest soils,
Forest hydrology,
Tree physiology,
Forest biomass, carbon, and bioenergy,
Forest biotechnology and molecular biology,
Forest Ecology,
Forest ecology,
Forest ecological services,
Restoration ecology,
Forest adaptation to climate change,
Wildlife ecology and management,
Silviculture and Forest Management,
Forest genetics and tree breeding,
Silviculture,
Forest RS, GIS, and modeling,
Forest management,
Forest Protection,
Forest entomology and pathology,
Forest fire,
Forest resources conservation,
Forest health monitoring and assessment,
Wood Science and Technology,
Wood Science and Technology.