Saithip Pakapongpan, Yingyot Poo-arporn, Sunatcha Ninket, Rungtiva P. Poo-arporn
{"title":"基于分子印迹聚合物与掺氮碳点-石墨烯纳米杂化技术的淀粉样β42 蛋白一次性电化学传感器","authors":"Saithip Pakapongpan, Yingyot Poo-arporn, Sunatcha Ninket, Rungtiva P. Poo-arporn","doi":"10.1016/j.microc.2024.111559","DOIUrl":null,"url":null,"abstract":"This work presented a molecularly imprinted polymer (MIP) electrochemical sensor for determination of amyloid-β42 (Aβ42) as an Alzheimer’s disease (AD) biomarker. A nitrogen-doped carbon-dot-graphene (NCD-G) nanohybrid was synthesized through a simple ultrasonic method. Polypyrrole (PPY) was used as an imprinted polymer with an Aβ42 template for the electropolymerization process. The NCD-G and MIP were modified on a screen-printed carbon electrode (SPCE) without binding agent. The morphology and electrochemical properties of MIP/NCD-G modified SPCE were studied by scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV–Vis spectroscopy, X-ray photoelectron spectroscopy (XPS), cyclic voltammetry (CV) and square wave voltammetry (SWV). The developed sensor exhibited good linear response in a range from 5 to 70 pg/mL with a limit of detection (LOD) of 1 pg/mL under optimal conditions. This sensor shows excellent performance due to the enhancement of effective surface area, electrical conductivity, and electrocatalytic activity. This MIP sensor takes advantage of molecularly imprinted technique combined with nanohybrid to achieve high selectivity and sensitivity. Moreover, it possessed good reproducibility and miniaturization that could be applied as a platform for portable devices in healthcare in the future. This work provides the combination of a zero-dimensional and two-dimensional materials, nitrogen doped carbon dots-graphene nanohybrid (NCD-G) by self-assembly through a simple ultrasonication method and applied as a molecularly imprinted polymer (MIP) sensor for determination of amyloid-β42 protein. We propose a minimum number of preparation steps which NCD-G could enhance the MIP sensor performance, owing to the synergistic effects with high selectivity and sensitivity. The hybrid formation of the carbon dots with graphene using their π-π interactions in carbon nanostructures. NCD-G nanohybrid and MIP modified on screen printed carbon electrode (SPCE) for the determination of amyloid-β42 have not been previously investigated. Moreover, this disposable strip is a single-use tool that might be essential for on-site devices.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.9000,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A disposable electrochemical sensor for amyloid-β42 protein based on molecular imprinted polymers with nitrogen doped carbon dots-graphene nanohybrid\",\"authors\":\"Saithip Pakapongpan, Yingyot Poo-arporn, Sunatcha Ninket, Rungtiva P. Poo-arporn\",\"doi\":\"10.1016/j.microc.2024.111559\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This work presented a molecularly imprinted polymer (MIP) electrochemical sensor for determination of amyloid-β42 (Aβ42) as an Alzheimer’s disease (AD) biomarker. A nitrogen-doped carbon-dot-graphene (NCD-G) nanohybrid was synthesized through a simple ultrasonic method. Polypyrrole (PPY) was used as an imprinted polymer with an Aβ42 template for the electropolymerization process. The NCD-G and MIP were modified on a screen-printed carbon electrode (SPCE) without binding agent. The morphology and electrochemical properties of MIP/NCD-G modified SPCE were studied by scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV–Vis spectroscopy, X-ray photoelectron spectroscopy (XPS), cyclic voltammetry (CV) and square wave voltammetry (SWV). The developed sensor exhibited good linear response in a range from 5 to 70 pg/mL with a limit of detection (LOD) of 1 pg/mL under optimal conditions. This sensor shows excellent performance due to the enhancement of effective surface area, electrical conductivity, and electrocatalytic activity. This MIP sensor takes advantage of molecularly imprinted technique combined with nanohybrid to achieve high selectivity and sensitivity. Moreover, it possessed good reproducibility and miniaturization that could be applied as a platform for portable devices in healthcare in the future. This work provides the combination of a zero-dimensional and two-dimensional materials, nitrogen doped carbon dots-graphene nanohybrid (NCD-G) by self-assembly through a simple ultrasonication method and applied as a molecularly imprinted polymer (MIP) sensor for determination of amyloid-β42 protein. We propose a minimum number of preparation steps which NCD-G could enhance the MIP sensor performance, owing to the synergistic effects with high selectivity and sensitivity. The hybrid formation of the carbon dots with graphene using their π-π interactions in carbon nanostructures. NCD-G nanohybrid and MIP modified on screen printed carbon electrode (SPCE) for the determination of amyloid-β42 have not been previously investigated. Moreover, this disposable strip is a single-use tool that might be essential for on-site devices.\",\"PeriodicalId\":391,\"journal\":{\"name\":\"Microchemical Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.9000,\"publicationDate\":\"2024-09-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchemical Journal\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.microc.2024.111559\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchemical Journal","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.microc.2024.111559","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
A disposable electrochemical sensor for amyloid-β42 protein based on molecular imprinted polymers with nitrogen doped carbon dots-graphene nanohybrid
This work presented a molecularly imprinted polymer (MIP) electrochemical sensor for determination of amyloid-β42 (Aβ42) as an Alzheimer’s disease (AD) biomarker. A nitrogen-doped carbon-dot-graphene (NCD-G) nanohybrid was synthesized through a simple ultrasonic method. Polypyrrole (PPY) was used as an imprinted polymer with an Aβ42 template for the electropolymerization process. The NCD-G and MIP were modified on a screen-printed carbon electrode (SPCE) without binding agent. The morphology and electrochemical properties of MIP/NCD-G modified SPCE were studied by scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV–Vis spectroscopy, X-ray photoelectron spectroscopy (XPS), cyclic voltammetry (CV) and square wave voltammetry (SWV). The developed sensor exhibited good linear response in a range from 5 to 70 pg/mL with a limit of detection (LOD) of 1 pg/mL under optimal conditions. This sensor shows excellent performance due to the enhancement of effective surface area, electrical conductivity, and electrocatalytic activity. This MIP sensor takes advantage of molecularly imprinted technique combined with nanohybrid to achieve high selectivity and sensitivity. Moreover, it possessed good reproducibility and miniaturization that could be applied as a platform for portable devices in healthcare in the future. This work provides the combination of a zero-dimensional and two-dimensional materials, nitrogen doped carbon dots-graphene nanohybrid (NCD-G) by self-assembly through a simple ultrasonication method and applied as a molecularly imprinted polymer (MIP) sensor for determination of amyloid-β42 protein. We propose a minimum number of preparation steps which NCD-G could enhance the MIP sensor performance, owing to the synergistic effects with high selectivity and sensitivity. The hybrid formation of the carbon dots with graphene using their π-π interactions in carbon nanostructures. NCD-G nanohybrid and MIP modified on screen printed carbon electrode (SPCE) for the determination of amyloid-β42 have not been previously investigated. Moreover, this disposable strip is a single-use tool that might be essential for on-site devices.
期刊介绍:
The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field.
Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.