有效利用皮肤微生物组特征进行指纹识别

IF 2 4区 医学 Q3 DERMATOLOGY
Seda Salman Yılmaz, Mert Ahmet Kuşkucu, Hüseyin Çakan, Gökhan Aygün
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引用次数: 0

摘要

背景最近的研究进展提高了包括皮肤微生物组在内的人类微生物组的重要性。尽管环境因素和个体差异在不断变化,但我们的目标是使用简单快速的分子方法来显示皮肤微生物群之间的人际差异。方法在非连续的 10 天内,我们从 10 个成年人身上采集了样本,并对每个皮肤样本进行了表皮葡萄球菌 16S 和 23S 基因的核型分析。此外,还对从 DNA 样本中获得的表皮葡萄球菌进行了 EcoRI 和 HindIII 酶反应以及变数串联重复(VNTR)反应。结果 在采集的环境样本中观察到,人们保留了皮肤微生物群的核心特征,并将其带到环境中。结论我们的研究表明,皮肤核心微生物群保留了其特征,并在环境中留下了痕迹。尽管存在环境差异,但个人微生物群仍保持不变,并具有特征性,这表明它可用于法医学,以区分不同的个体。这些采用简单快速方法得出的结果进一步提高了这项研究的重要性和意义。研究结果表明,个人皮肤微生物群可以提高准确性和可靠性,特别是在法医分析中,从而为刑事调查做出重大贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effective use of skin microbiome signatures for fingerprint identification
BackgroundRecent advances have increased the importance of the human microbiome, including the skin microbiome. Despite the hand microbiome research, the factors affecting the composition of the hand microbiome and their personal characteristics are incompletely known.ObjectivesDespite changing environmental factors and personal variation, we aimed to indicate the interpersonal distinction between skin microbiota using simple and rapid molecular methods.MethodsOver a non‐consecutive 10‐day period, samples were taken from 10 adult individuals, and ribotyping analysis of the 16S and 23S genes of S. epidermidis was performed on each skin sample. Additionally, EcoRI and HindIII enzyme reactions and variable number tandem repeat (VNTR) reactions of S. epidermidis obtained from DNA samples were performed. The skin microbiomes of individuals were evaluated along with the microbiome profiles left on the surfaces they touched.ResultsIn the environmental samples taken, it has been observed that people preserve their core skin microbiota characters and carry them to their environment. It was determined that the highest similarity rate was 77.14%, and the lowest similarity rate was 31.74%.ConclusionOur study showed that the core skin microbiota retains its characteristics and leaves traces in environments. The fact that the personal microbiome remains unchanged despite environmental differences and has characteristic features has shown that it can be used in forensic sciences to distinguish individuals from each other. These results with simple and rapid methods further increased the importance and significance of the study. The findings indicate that personal skin microbiota can provide a significant contribution to criminal investigations by increasing accuracy and reliability, especially in forensic analyses.
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来源期刊
Skin Research and Technology
Skin Research and Technology 医学-皮肤病学
CiteScore
3.30
自引率
9.10%
发文量
95
审稿时长
6-12 weeks
期刊介绍: Skin Research and Technology is a clinically-oriented journal on biophysical methods and imaging techniques and how they are used in dermatology, cosmetology and plastic surgery for noninvasive quantification of skin structure and functions. Papers are invited on the development and validation of methods and their application in the characterization of diseased, abnormal and normal skin. Topics include blood flow, colorimetry, thermography, evaporimetry, epidermal humidity, desquamation, profilometry, skin mechanics, epiluminiscence microscopy, high-frequency ultrasonography, confocal microscopy, digital imaging, image analysis and computerized evaluation and magnetic resonance. Noninvasive biochemical methods (such as lipids, keratin and tissue water) and the instrumental evaluation of cytological and histological samples are also covered. The journal has a wide scope and aims to link scientists, clinical researchers and technicians through original articles, communications, editorials and commentaries, letters, reviews, announcements and news. Contributions should be clear, experimentally sound and novel.
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