海洋微藻 Tetraselmis sp.叶绿素提取物对寨卡病毒感染的抗病毒活性

IF 4.9 2区医学 Q1 CHEMISTRY, MEDICINAL

Marine Drugs Pub Date : 2024-08-31 DOI:10.3390/md22090397

Nalae Kang, Eun-A Kim, Areumi Park, Seong-Yeong Heo, Jun-Ho Heo, Won-Kyu Lee, Yong-Kyun Ryu, Soo-Jin Heo

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引用次数: 0

摘要

最近，韩国在大规模培育四膜藻方面取得了进展，实现了这种海洋微藻的全年生产。本研究通过研究四膜藻提取物和主要成分的抗病毒特性，探索四膜藻生物质的潜在工业应用。在寨卡病毒（ZIKV）感染的细胞中评估了四膜藻提取物的抗病毒效果。经过广泛的分离和纯化，利用液相色谱-质谱（LC-MS）和核磁共振（NMR）分析对主要化合物进行了表征。这些化合物的抗病毒活性已通过体外和硅学测试得到证实。Tetraselmis sp.提取物降低了ZIKV感染细胞中传染性病毒颗粒和非结构蛋白1信使RNA的水平，而不会引起细胞毒性。此外，它们还能调节干扰素介导的免疫系统反应。Tetraselmis sp.提取物主要由四种叶绿素组成：叶绿素a、叶绿素e6-131-152-二甲基-173-phytyl酯、羟基叶绿素a和羟基叶绿素a。其中，叶绿素 a、叶绿素 e6-131-152-二甲基-173-phytyl 酯和羟基叶绿素在 ZIKV 感染细胞中显示出抗病毒活性，分子对接模拟预测了这些叶绿素与 ZIKV 之间的相互作用。我们的研究结果表明，Tetraselmis sp.叶绿素提取物对ZIKV具有抗病毒作用，可作为治疗ZIKV感染的潜在候选药物。

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Antiviral Activity of Chlorophyll Extracts from Tetraselmis sp., a Marine Microalga, Against Zika Virus Infection

Recent advancements in the large-scale cultivation of Tetraselmis sp. in Korea have enabled year-round production of this marine microalgae. This study explores the potential industrial applications of Tetraselmis sp. biomass by investigating the antiviral properties of its extracts and primary components. The antiviral effects of Tetraselmis sp. extracts were evaluated in Zika virus (ZIKV)-infected cells. Following extensive isolation and purification, the main compounds were characterized using liquid chromatography–mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) analyses. Their antiviral activities were confirmed using in vitro and in silico tests. Tetraselmis sp. extracts reduced infectious viral particles and non-structural protein 1 messenger RNA levels in ZIKV-infected cells without inducing cytotoxicity. Additionally, they modulated the interferon-mediated immune system responses. Tetraselmis sp. extracts are composed of four main chlorophylls: chlorophyll a, chlorin e6-131-152-dimethyl-173-phytyl ester, hydroxychlorophyll a, and hydroxypheophytin a. Among them, chlorophyll a, chlorin e6-131-152-dimethyl-173-phytyl ester, and hydroxypheophytin showed the antiviral activities in ZIKV-infected cells and molecular docking simulations predicted interactions between these chlorophylls and ZIKV. Our findings suggest that Tetraselmis sp. chlorophyll extracts exert antiviral effects against ZIKV and could serve as potential therapeutic candidates against ZIKV infection.

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来源期刊

Marine Drugs 医学-医药化学

CiteScore

9.60

自引率

14.80%

发文量

671

审稿时长

1 months

期刊介绍： Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.