Lei Li, Jing Huang, Lixin Feng, Liyan Xu, Houwen Lin, Kechun Liu, Xiaobin Li, Rongchun Wang
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引用次数: 0
摘要
Altechromone A 又称 2,5-二甲基-7-羟基色酮,是一种含有一个羟基和一个酮基的羟基酮。在这项研究中,我们从海洋源真菌青霉(Penicillium Chrysogenum,XY-14-0-4)中分离出了 Altechromone A。以前的报告显示,Altechromone A 具有多种活性,包括抑制肿瘤、抗菌和抗病毒活性。然而,目前还没有关于其在炎症性肠病(IBD)中抗炎活性的研究。在此,我们利用斑马鱼模型评估了其抗炎活性,尤其是在 IBD 中的抗炎活性及其潜在机制。我们的研究结果表明,Altechromone A 在 CuSO4、尾切和 LPS 诱导的斑马鱼炎症模型中分别具有抗炎活性。此外,在 TNBS 诱导的 IBD 斑马鱼模型中,Altechromone A 还能大大减少中性粒细胞的数量,改善肠道蠕动和流出效率,减轻肠道损伤,减少活性氧的产生。转录组学测序和实时 qPCR 表明,Altechromone A 可抑制 TNF-α、NF-κB、IL-1、IL-1β、IL-6 和 NLRP3 等促炎基因的表达。因此,这些数据表明,Altechromone A 通过抑制炎症反应对 IBD 具有治疗作用。
Altechromone A Ameliorates Inflammatory Bowel Disease by Inhibiting NF-κB and NLRP3 Pathways
Altechromone A, also known as 2,5-dimethyl-7-hydroxychromone, is a hydroxyketone containing one hydroxyl and one ketone group. In this study, we isolated Altechromone A from the marine-derived fungus Penicillium Chrysogenum (XY-14-0-4). Previous reports show that Altechromone A has various activities including tumor suppression, antibacterial, and antiviral activities. However, there is no study about its anti-inflammatory activity in inflammatory bowel disease (IBD). Here, we assess the anti-inflammatory activity, especially in IBD, and its potential mechanism using the zebrafish model. Our results indicated that Altechromone A has anti-inflammatory activity in a CuSO4-, tail-cutting-, and LPS-induced inflammatory model in zebrafish, respectively. In addition, Altechromone A greatly reduced the number of neutrophils, improved intestinal motility and efflux efficiency, alleviated intestinal damage, and reduced reactive oxygen species production in the TNBS-induced IBD zebrafish model. The transcriptomics sequencing and real-time qPCR indicated that Altechromone A inhibited the expression of pro-inflammatory genes including TNF-α, NF-κB, IL-1, IL-1β, IL-6, and NLRP3. Therefore, these data indicate that Altechromone A exhibits therapeutic effects in IBD by inhibiting the inflammatory response.
期刊介绍:
Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.