基于蛋白质组学、生物化学和肽组学的分析揭示了芥蓝幼苗的热响应变化

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Reema Rani, Ibandalin Mawlong, Balbeer Balbeer, M.S. Sujith Kumar , Pramod Kumar Rai, Vijay Veer Singh
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引用次数: 0

摘要

幼苗期的热胁迫对印度芥菜的生长和产量有着至关重要的影响。鉴定热胁迫响应蛋白对了解热胁迫适应机制至关重要。在这项工作中,研究了耐热基因型 BPR 543-2 在芥菜早期幼苗热胁迫处理下的生化、蛋白质组和肽组学响应。通过基于 LC-MS/MS 的分析,发现在热胁迫的 0、4 和 8 小时内分别有 403、328 和 369 个蛋白质专门表达,而在 0-4 小时、4-8 小时和 0-8 小时内分别有 89、119 和 81 个蛋白质差异积累。值得注意的是,BPR 543-2 表达的热休克蛋白、伴侣蛋白、参与碳水化合物和能量代谢的酶、细胞壁修饰和转录因子水平升高。此外,利用 MALDI-TOF-MS,还发现在热胁迫的不同时间点,参与 DNA 修复、信号转导和代谢适应的蛋白质过量表达。此外,生化分析表明,受胁迫样品中的光合色素TAC、TF较高,湍流较小。通过生化、蛋白质组学和肽组学方法的综合分析,观察到 BPR 543-2 对热胁迫的抗逆性更强,在胁迫样品中经历的显著代谢紊乱更少,表明其在幼苗期对热胁迫的适应性更强。对丰度不同的蛋白质进行了功能注释,以确定其亚细胞定位、生物和分子功能。这项工作证明了基于蛋白质组学和肽组学方法的实用性,为了解印度芥菜的热胁迫适应机制提供了新的视角。所发现的关键蛋白为开发热敏性黄铜作物的抗逆性提供了令人感兴趣的目标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Proteomic, biochemical and peptidomics based analysis reveals heat responsive changes in the seedlings of Brassica juncea

Proteomic, biochemical and peptidomics based analysis reveals heat responsive changes in the seedlings of Brassica juncea

Heat stress at seedling stage has a crucial impact on Indian mustard growth and productivity. Identifying heat stress responsive proteins can be crucial to understand the heat stress adaptive mechanisms. In this work, biochemical, proteomic, and peptidomics response of the thermotolerant genotype, BPR 543-2 was investigated in the early seedlings of mustard under heat stress treatment. A total of 403, 328 and 369 number of proteins were identified to be expressed exclusively during 0, 4 and 8 h of heat-stress while 89, 119 and 81 were differentially accumulated during 0–4 h, 4 h-8 h and 0–8 h using LC–MS/MS based analysis. Notably, BPR 543-2 expressed elevated levels of heat shock proteins, chaperones, enzymes involved in the metabolism of carbohydrate and energy, cell wall modifications and transcription factors. In addition, using MALDI-TOF-MS, overexpressed proteins involved in DNA repair, signal transduction and metabolic adaptation were identified during different time point of heat stress. Moreover, biochemical analysis revealed high TAC, TF and less turbulence in photosynthetic pigments in stressed samples. Through combined analysis of biochemical, proteomics and peptidomics approaches, it was observed that BPR 543-2 was more resilient to heat stress and experienced fewer significant metabolic disruptions in stressed samples, demonstrating its adaptability to heat stress at early seedling stage. The proteins with differential abundance were functionally annotated in-silico for their subcellular localization, biological and molecular functions. This work demonstrates the usefulness of proteomics and peptidomics-based approaches by providing fresh insights into the mechanism behind the heat-stress adaption mechanisms in Indian mustard. The identified critical proteins provide intriguing targets for developing stress tolerance in heat-sensitive brassica crops.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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