Yi Qian, Ziao Hu, Zhuoya Cheng, Jun Tao, Daqiu Zhao
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引用次数: 0
摘要
草本牡丹(Paeonia lactiflora Pall.)它喜冷凉气候,但在长江中下游地区不耐夏季高温。此前,我们发现过氧化物酶(POD)是一种重要的抗氧化酶,在乳白花椰菜耐高温过程中发挥着重要作用。本研究分离了候选基因 PlPOD45,并验证了其在抗高温胁迫中的功能。结果表明,PlPOD45的开放阅读框为978 bp,编码325个氨基酸。其蛋白质定位于细胞膜和细胞质。高温胁迫诱导了 PlPOD45 的表达。异源过表达 PlPOD45 提高了植物对高温胁迫的耐受性,降低了活性氧(ROS)积累、相对电导率和丙二醛含量,增加了可变荧光与最高荧光的比率和 POD 活性。相反,当乳花草暴露于高温胁迫时,沉默 PlPOD45 可降低其 POD 活性、ROS 清除能力和细胞膜稳定性。这些结果表明,PlPOD45通过清除ROS对耐高温性具有正向调节作用,这将为提高乳花草的耐高温性提供理论依据。
PlPOD45 positively regulates high-temperature tolerance of herbaceous peony by scavenging reactive oxygen species
Herbaceous peony (Paeonia lactiflora Pall.) is a widely used famous traditional flower in China. It prefers cold and cool climate, but is not resistant to high temperature during summer in the middle and lower reaches of the Yangtze River. Previously, we found peroxidase (POD) is an important antioxidant enzyme that played an important role in high-temperature tolerance of P. lactiflora. The present study isolated the candidate gene PlPOD45 and verified its function in resisting high-temperature stress. And the results showed that PlPOD45 had an open reading frame of 978 bp that encoded 325 amino acids. Its protein was localized to the cell membrane and cytoplasm. High-temperature stress induced PlPOD45 expression. Heterologous overexpression of PlPOD45 improved plant tolerance to high-temperature stress, decreased reactive oxygen species (ROS) accumulation, relative electrical conductivity and malondialdehyde content, and increased the ratio of variable fluorescence to highest fluorescence and POD activity. Conversely, silencing PlPOD45 in P. lactiflora could decrease POD activity, ROS scavenging capability and cell membrane stability when these plants were exposed to high-temperature stress. These results suggest that PlPOD45 positively regulates high-temperature tolerance through ROS scavenging, which would provide a theoretical basis for improving high-temperature tolerance in P. lactiflora.
期刊介绍:
Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.