小反刍动物蜱虫体内阿纳普拉原虫、波莱氏阿纳普拉原虫和立克次体的分子检测

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

BMC Veterinary Research Pub Date : 2024-09-11 DOI:10.1186/s12917-024-04259-6

Zaibullah Khan, Farman Ullah, Shafi Ullah, Mohammed Ibrahim, Momin Khan, Gauhar Rehman, Tetsuya Tanaka, Mashal M. Almutairi, Abdulaziz Alouffi, Abid Ali

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引用次数: 0

摘要

阿纳普拉斯菌属和立克次体属是细胞内病媒传播的病原体，由多种蜱虫和脊椎动物宿主携带。本研究旨在对从巴基斯坦开伯尔-普赫图赫瓦（KP）九个地区的家畜宿主处收集到的不同蜱虫中的阿纳普拉斯马属和立克次体进行分子鉴定。从牛、山羊和绵羊身上共采集到 862 只蜱虫。牛的蜱虫感染率最高，为 56.14%（32/57），其次是山羊的 45.45%（40/88）和绵羊的 42.05%（45/107）。Rhipicephalus microplus（305/862，35.38%）是主要种类，其次是 Haemaphysalis sulcata（243/862，28.19%）、Hyalomma anatolicum（133/862，15.42%）、Haemaphysalis bispinosa（120/862，13.92%）和 Hyalomma kumari（61/862，7.07%）。根据 16 S rDNA 和外膜蛋白 A（ompA）部分片段的扩增结果，分别对 135 只蜱进行了阿纳普拉斯马属和立克次体的筛查。在 Ha. bispinosa 和 Ha. sulcata 中检测到的 16 S rDNA 序列与 Anaplasma bovis 的一致性为 99.98%，而在 Rh. microplus 中检测到的序列与 Candidatus Anaplasma boleense 的一致性为 99.84%。同样，在 Ha. sulcata 中检测到的 ompA 序列与 Rickettsia sp.的同一性为 100%，与 Rickettsia slovaca 的同一性为 97.93%，而在 Rh. microplus 中检测到的另一个序列与 Candidatus Rickettsia shennongii 的同一性为 100%。在系统发生树中，这些序列与巴基斯坦、中国、土耳其、韩国、南非和黑塞哥维那的相应物种聚类。这是首次研究报告在 Ha. bispinosa 和 Ha. sulcata、Ca.A.boleense，以及在 Ha. sulcata 中检测到 R. slovaca-like。我们的研究结果表明，有必要定期监测该地区家畜硬蜱中的立克次体。

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Molecular detection of Anaplasma bovis, Candidatus Anaplasma boleense and Rickettsia spp. in ticks infesting small ruminants

Anaplasma spp. and Rickettsia spp. are intracellular vector-borne pathogens and harbored by a wide range of ticks and vertebrate hosts. Aim of this study was to molecularly characterize Anaplasma spp. and Rickettsia spp. in different ticks collected from livestock hosts in nine districts of Khyber Pakhtunkhwa (KP), Pakistan. In total, 862 ticks were collected from cattle, goats and sheep. Highest tick’s infestation was observed on cattle 56.14% (32/57), followed by goats 45.45% (40/88), and sheep 42.05% (45/107). Rhipicephalus microplus (305/862, 35.38%) was predominant species, followed by Haemaphysalis sulcata (243/862, 28.19%), Hyalomma anatolicum (133/862, 15.42%), Haemaphysalis bispinosa (120/862, 13.92%), and Hyalomma kumari (61/862, 7.07%). A subset of 135 ticks were screened for Anaplasma spp. and Rickettsia spp. based on the amplification of partial 16 S rDNA and outer-membrane protein A (ompA) fragments, respectively. In total, 16 ticks (11.85%) were positive for Anaplasma spp. and Rickettsia spp. Obtained 16 S rDNA sequences for Anaplasma spp. detected in Ha. bispinosa and Ha. sulcata showed 99.98% identity with Anaplasma bovis, while other detected in Rh. microplus showed 99.84% identity with Candidatus Anaplasma boleense. Similarly, detected ompA sequence in Ha. sulcata showed 100% identity with Rickettsia sp. and 97.93% with Rickettsia slovaca, and another sequence detected in Rh. microplus showed 100% identity with Candidatus Rickettsia shennongii. In phylogenetic trees, these sequences clustered with corresponding species from Pakistan, China, Turkey, South Korea, South Africa, and Herzegovina. This is the first study reporting detection of A. bovis in Ha. bispinosa and Ha. sulcata, Ca. A. boleense in Rh. microplus collected from goats, and R. slovaca-like in Ha. sulcata. Our results enforce the need for regular surveillance of Rickettsiales in hard ticks infesting livestock in the region.

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来源期刊

BMC Veterinary Research VETERINARY SCIENCES-

CiteScore

4.80

自引率

3.80%

发文量

420

审稿时长

3-6 weeks

期刊介绍： BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.