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引用次数: 0
摘要
马氏反应是糖与蛋白质氨基之间的反应。在这里，Nα-乙酰赖氨酸（Ac-Lys）和果糖是模型化合物。利用紫外可见吸收、荧光和 785 纳米激发非共振拉曼光谱获得了 pH 值为 7.4、温度为 37°C 的反应中间产物和产物的结构信息。在 6 小时的反应中，光谱数据中的三个独立成分分别是溶解的反应物（A）、它们的复合物（B）和共价键产物（C）。从 A 到 B 和从 B 到 C 的组分变化速率常数分别为 (9.9 ± 0.1) × 10-3 和 (3.3 ± 0.1) × 10-3 min-1。聚合物马氏反应产物尚未形成。根据光谱数据讨论了各状态下溶质分子的结构。
Spectroscopic Analysis of the Initial Step of the Maillard Reaction of d-Fructose and Nα-Acetyl-l-Lysine
The Maillard reaction is a reaction between sugar and the amino group of proteins. Herein, Nα-acetyl lysine (Ac-Lys) and fructose were the model compounds. Structural information of the intermediates and products of the reaction at pH 7.4 and 37°C was obtained using UV–Vis absorption, fluorescence, and 785-nm excited non-resonance Raman spectroscopy. In the 6-h reaction, three independent components in the spectroscopic data were attributed to the solvated reactants (A), their complex (B), and a covalent-bonded product (C). The rate constant of the change in the fraction from A to B and B to C were (9.9 ± 0.1) × 10−3 and (3.3 ± 0.1) × 10−3 min−1, respectively. Polymeric Maillard reaction products were not yet formed. The structures of the solute molecules in each state were discussed based on the spectroscopic data.
期刊介绍：
The Journal of Raman Spectroscopy is an international journal dedicated to the publication of original research at the cutting edge of all areas of science and technology related to Raman spectroscopy. The journal seeks to be the central forum for documenting the evolution of the broadly-defined field of Raman spectroscopy that includes an increasing number of rapidly developing techniques and an ever-widening array of interdisciplinary applications.
Such topics include time-resolved, coherent and non-linear Raman spectroscopies, nanostructure-based surface-enhanced and tip-enhanced Raman spectroscopies of molecules, resonance Raman to investigate the structure-function relationships and dynamics of biological molecules, linear and nonlinear Raman imaging and microscopy, biomedical applications of Raman, theoretical formalism and advances in quantum computational methodology of all forms of Raman scattering, Raman spectroscopy in archaeology and art, advances in remote Raman sensing and industrial applications, and Raman optical activity of all classes of chiral molecules.
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