Fu-Long Lin , Kizerbo A. Taizoumbe , Yi-Xuan Wang , Jia-Hua Huang , Gao-Qian Wang , Guo-Dong Chen , Jian-Ming Lv , Dan Hu , Hao Gao , Jeroen S. Dickschat
{"title":"参与塔拉罗-7,13-二烯生物合成的真菌镰刀菌烷型二萜合成酶的机理特征","authors":"Fu-Long Lin , Kizerbo A. Taizoumbe , Yi-Xuan Wang , Jia-Hua Huang , Gao-Qian Wang , Guo-Dong Chen , Jian-Ming Lv , Dan Hu , Hao Gao , Jeroen S. Dickschat","doi":"10.1039/d4ob01348d","DOIUrl":null,"url":null,"abstract":"<div><div>The cyclisation mechanism of the fungal fusicoccane (FC)-type diterpene synthase (DTS) TadA was investigated by extensive isotopic labelling experiments, and the pH-dependency of the product selectivity of this enzyme was explored. These studies provide new insights into the cyclisation mechanisms of FC-type DTSs.</div></div>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mechanistic characterisation of a fungal fusicoccane-type diterpene synthase involved in the biosynthesis of talaro-7,13-diene†\",\"authors\":\"Fu-Long Lin , Kizerbo A. Taizoumbe , Yi-Xuan Wang , Jia-Hua Huang , Gao-Qian Wang , Guo-Dong Chen , Jian-Ming Lv , Dan Hu , Hao Gao , Jeroen S. Dickschat\",\"doi\":\"10.1039/d4ob01348d\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The cyclisation mechanism of the fungal fusicoccane (FC)-type diterpene synthase (DTS) TadA was investigated by extensive isotopic labelling experiments, and the pH-dependency of the product selectivity of this enzyme was explored. These studies provide new insights into the cyclisation mechanisms of FC-type DTSs.</div></div>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-08-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/org/science/article/pii/S1477052024008024\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/org/science/article/pii/S1477052024008024","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0
摘要
通过广泛的同位素标记实验研究了真菌菲卡烷（FC）型二萜合成酶（DTS）TadA的环化机制，并探讨了该酶的产物选择性与 pH 值的关系。这些研究为了解 FC 型二萜合成酶的环化机制提供了新的视角。
Mechanistic characterisation of a fungal fusicoccane-type diterpene synthase involved in the biosynthesis of talaro-7,13-diene†
The cyclisation mechanism of the fungal fusicoccane (FC)-type diterpene synthase (DTS) TadA was investigated by extensive isotopic labelling experiments, and the pH-dependency of the product selectivity of this enzyme was explored. These studies provide new insights into the cyclisation mechanisms of FC-type DTSs.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
