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引用次数: 0
摘要
通过比较年轻样本和老年样本的转录组图谱，可以发现与衰老有关的基因，并深入了解受衰老影响的生物功能。最近的研究发现了基因表达中与性别、组织和细胞类型特异性相关的年龄变化。这项研究报告了相反衰老效应的整体情况，即衰老会增加一个细胞亚群的基因表达，而减少另一个细胞亚群的基因表达。我们利用大型公开小鼠单细胞 RNA 测序数据集 Tabula Muris Senis 数据集，比较了衰老对不同细胞亚群的影响。结果，在基因中广泛观察到了相反的衰老效应，尤其是富集在与核糖体功能和翻译相关的基因中。在已知的衰老相关基因中也观察到了相反的衰老效应。此外，通过表达基因数量和香农熵量化的转录组多样性也观察到了相反的衰老效应。这项研究强调了在干预衰老相关基因时考虑细胞亚群的重要性。
The opposite aging effect to single cell transcriptome profile among cell subsets
Comparing transcriptome profiling between younger and older samples reveals genes related to aging and provides insight into the biological functions affected by aging. Recent research has identified sex, tissue, and cell type-specific age-related changes in gene expression. This study reports the overall picture of the opposite aging effect, in which aging increases gene expression in one cell subset and decreases it in another cell subset. Using the Tabula Muris Senis dataset, a large public single-cell RNA sequencing dataset from mice, we compared the effects of aging in different cell subsets. As a result, the opposite aging effect was observed widely in the genes, particularly enriched in genes related to ribosomal function and translation. The opposite aging effect was observed in the known aging-related genes. Furthermore, the opposite aging effect was observed in the transcriptome diversity quantified by the number of expressed genes and the Shannon entropy. This study highlights the importance of considering the cell subset when intervening with aging-related genes.
期刊介绍：
The journal Biogerontology offers a platform for research which aims primarily at achieving healthy old age accompanied by improved longevity. The focus is on efforts to understand, prevent, cure or minimize age-related impairments.
Biogerontology provides a peer-reviewed forum for publishing original research data, new ideas and discussions on modulating the aging process by physical, chemical and biological means, including transgenic and knockout organisms; cell culture systems to develop new approaches and health care products for maintaining or recovering the lost biochemical functions; immunology, autoimmunity and infection in aging; vertebrates, invertebrates, micro-organisms and plants for experimental studies on genetic determinants of aging and longevity; biodemography and theoretical models linking aging and survival kinetics.
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