二极管激光和氦氖激光辐照对减轻花青素生产红肉苹果细胞悬浮液中营养缺乏引起的损伤的激发效应。

IF 2.1 4区 医学 Q2 BIOLOGY
Hashem Kazemzadeh-Beneh,Ebrahim Safari,Fariborz Zaare-Nahandi,Nasser Mahna
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引用次数: 0

摘要

目的我们探讨了在连续生产花青素的过程中,激光辐照对减轻花青素生产红肉苹果细胞悬浮液中营养缺乏引起的损伤的诱导作用。方法用 4 种强度的红色氦氖激光(RHNL)和蓝色二极管激光(BDL)辐照花青素生产红肉苹果细胞 20 分钟。结果营养素缺乏对光照对照组的总可溶性蛋白(TSP)、超氧化物歧化酶(SOD)活性和总酚含量(TPC)有负面影响,而对光照对照组的丙二醛(MDA)、总黄酮含量(TFC)、O2-、H2O2-、脂氧合酶(LOX)和多酚氧化酶(PPO)活性有正面影响,说明存在氧化应激。激光照射悬浮细胞对测量参数的影响各不相同,且与生长时间、强度水平和激光类型有关。同样,激光的激发效应依赖于 ROS 生成和抗氧化系统之间的临界阈值,该阈值决定了细胞对抗氧化应激的命运。强度为 6.46 mWcm-2 的 RHNL 和强度为 13.73 mWcm-2 的 BDL 也显示了同样的趋势。这些强度导致 SOD、APX、POD 和 CAT 活性以及 TSP、TPC、TFC、脯氨酸和甘氨酸甜菜碱积累显著增加,同时诱导 LOX 和 PPO 活性以及 MDA 和 ROS 生成减少,通过减少脂质过氧化和细胞膜氧化损伤减轻了长期营养缺乏对细胞的伤害。结论结果表明,激光在缓解营养缺乏应激方面的应用依赖于在 ROS 生成和抗氧化系统之间建立适当的平衡,从而使营养缺乏的花青素生成细胞能够持续生成花青素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The elicitation effects of diode and He-Ne laser irradiations on the alleviation of nutrient-deficiency induced damage in anthocyanin-producing red-fleshed apple cell suspension.
PURPOSE We explored the elicitation role of the laser irradiations on the alleviation of nutrient-deficiency induced damage in anthocyanin-producing red-fleshed apple cell suspension in continuous production of anthocyanin. METHODS Anthocyanin-producing red-fleshed apple cells were irradiated by 4 intensity levels of red He-Ne (RHNL) and blue diode (BDL) lasers for 20 min. RESULTS Nutrient deficiency indicated negative effect on total soluble proteins (TSP), superoxidase dismutase (SOD) activity, and total phenolics content (TPC) while it displayed a positive effect on malondialdehyde (MDA), total flavonoids content (TFC), O2-, H2O2-, and lipoxygenase (LOX) and polyphenol oxidase (PPO) activities in light controls, illustrating oxidative stress. The laser irradiations on suspension cells indicated variable effects on measured parameters and were time of growth-, levels of intensity-, and laser type-dependent. Likewise, the elicitation effects of lasers relied on a critical threshold among ROS generation and antioxidative system which determines the fate of cells against oxidative stress. The same trend was displayed by RHNL at 6.46 mWcm-2 intensity and BDL at 13.73 mWcm-2. These intensities resulted in a significant increase in SOD, APX, POD, and CAT activities and TSP, TPC, TFC, proline, and glycine betaine accumulation, while induced decrease in LOX, and PPO activities and MDA, and ROS generation, alleviating cellular injury from prolonged nutrient deficiency by diminishing lipid peroxidation and oxidative damages of cell membrane. CONCLUSION Results suggested that lasers application on mitigating nutrient deficiency stress relied on establishing a suitable balance between ROS generation and antioxidative system, which enables the nutrient-starved anthocyanin-producing cells to continuously produce anthocyanin.
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来源期刊
CiteScore
5.00
自引率
11.50%
发文量
142
审稿时长
3 months
期刊介绍: The International Journal of Radiation Biology publishes original papers, reviews, current topic articles, technical notes/reports, and meeting reports on the effects of ionizing, UV and visible radiation, accelerated particles, electromagnetic fields, ultrasound, heat and related modalities. The focus is on the biological effects of such radiations: from radiation chemistry to the spectrum of responses of living organisms and underlying mechanisms, including genetic abnormalities, repair phenomena, cell death, dose modifying agents and tissue responses. Application of basic studies to medical uses of radiation extends the coverage to practical problems such as physical and chemical adjuvants which improve the effectiveness of radiation in cancer therapy. Assessment of the hazards of low doses of radiation is also considered.
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