Anna Rosa Garbuglia, Gjergji Koja, Umbertina Villano, Claudia Minosse, Michele Equestre, Silvia Pauciullo, Antonio Coppola, Elisabetta Madonna, Giovanna Picchi, Jessica Di Biase, Margherita Dalessandro, Anna Rughetti, Katia Casinelli, Barbara Camilloni, Rinalda Mariani, Alessandro Grimaldi, Anna Rita Ciccaglione, Roberto Bruni
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Aim of the study was the molecular characterization of HEV from autochthonous hepatitis E cases occurred in Abruzzo and Lazio between 2015 and 2023.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>Samples from 118 cases collected as part of virological surveillance in Abruzzo and Lazio from 2015 to 2023 were subjected to HEV sequencing and phylogenetic analysis.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>The main observed subtype was 3f, followed by 3c and 3e. The annual subtype distribution was quite stable over the observation period, but 3f cases tended to concentrate in winter/early spring whereas 3e cases in summer. Phylogenetic clusters of highly related sequences (a) highlighted unrecognized “point source outbreaks”, (b) provided molecular support to temporally and/or geographically linked cases and (c) provided evidence for transmission of identical/highly related strains up to months/years following their first detection.</p><h3 data-test=\"abstract-sub-heading\">Conclusions</h3><p>The data provide an overview of the HEV strains responsible for human infections over eight years in Central Italy. The observed subtype distribution appears to agree better with the subtype distribution reported in Italy in pigs rather than in geographically matched wild boars, suggesting pig and its derivate food was a more frequent source of infection than wild boar in Abruzzo and Lazio. 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引用次数: 0
摘要
目的 HEV 是欧洲一种新出现的病原体，以前曾在意大利中部阿布鲁佐和拉齐奥地区出现过高流行率。以前没有报道过对意大利中部地区数年来造成人类感染的 HEV 株系进行系统分析。该研究的目的是对 2015 年至 2023 年期间阿布鲁佐和拉齐奥地区发生的自源性戊型肝炎病例中的 HEV 进行分子定性。在观察期间，每年的亚型分布相当稳定，但 3f 病例往往集中在冬季/早春，而 3e 病例则集中在夏季。高度相关序列的系统发育群(a)突显了未被发现的 "点源爆发"，(b)为时间和/或地理上相关的病例提供了分子支持，(c)为相同/高度相关菌株在首次发现后长达数月/数年的传播提供了证据。观察到的亚型分布似乎更符合意大利报告的猪亚型分布，而不是地理匹配的野猪亚型分布，这表明在阿布鲁佐和拉齐奥，猪及其衍生食物是比野猪更常见的感染源。分子特征描述对于识别 "点源爆发 "和监测 HEV 循环至关重要。
HEV-3 subtypes and strains detected in cases of HEV infection in central Italy from 2015 to 2023
Purpose
HEV is an emerging pathogen in Europe and was previously shown to be hyperendemic in areas of Abruzzo and Lazio, Central Italy. No systematic analysis of the HEV strains responsible for human infections over several years in Central Italy has previously been reported. Aim of the study was the molecular characterization of HEV from autochthonous hepatitis E cases occurred in Abruzzo and Lazio between 2015 and 2023.
Methods
Samples from 118 cases collected as part of virological surveillance in Abruzzo and Lazio from 2015 to 2023 were subjected to HEV sequencing and phylogenetic analysis.
Results
The main observed subtype was 3f, followed by 3c and 3e. The annual subtype distribution was quite stable over the observation period, but 3f cases tended to concentrate in winter/early spring whereas 3e cases in summer. Phylogenetic clusters of highly related sequences (a) highlighted unrecognized “point source outbreaks”, (b) provided molecular support to temporally and/or geographically linked cases and (c) provided evidence for transmission of identical/highly related strains up to months/years following their first detection.
Conclusions
The data provide an overview of the HEV strains responsible for human infections over eight years in Central Italy. The observed subtype distribution appears to agree better with the subtype distribution reported in Italy in pigs rather than in geographically matched wild boars, suggesting pig and its derivate food was a more frequent source of infection than wild boar in Abruzzo and Lazio. Molecular characterization is essential to recognize “point source outbreaks” and to monitor HEV circulation.
期刊介绍：
Infection is a journal dedicated to serving as a global forum for the presentation and discussion of clinically relevant information on infectious diseases. Its primary goal is to engage readers and contributors from various regions around the world in the exchange of knowledge about the etiology, pathogenesis, diagnosis, and treatment of infectious diseases, both in outpatient and inpatient settings.
The journal covers a wide range of topics, including:
Etiology: The study of the causes of infectious diseases.
Pathogenesis: The process by which an infectious agent causes disease.
Diagnosis: The methods and techniques used to identify infectious diseases.
Treatment: The medical interventions and strategies employed to treat infectious diseases.
Public Health: Issues of local, regional, or international significance related to infectious diseases, including prevention, control, and management strategies.
Hospital Epidemiology: The study of the spread of infectious diseases within healthcare settings and the measures to prevent nosocomial infections.
In addition to these, Infection also includes a specialized "Images" section, which focuses on high-quality visual content, such as images, photographs, and microscopic slides, accompanied by brief abstracts. This section is designed to highlight the clinical and diagnostic value of visual aids in the field of infectious diseases, as many conditions present with characteristic clinical signs that can be diagnosed through inspection, and imaging and microscopy are crucial for accurate diagnosis. The journal's comprehensive approach ensures that it remains a valuable resource for healthcare professionals and researchers in the field of infectious diseases.
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