养鸭场分离出的大肠埃希菌中,ISVsa3-ORF2-abh-tet(X4)循环中间传播对替加环素的耐药性

IF 4.6 2区 医学 Q2 IMMUNOLOGY
Chao Jiang, Jie Yang, Gang Xiao, Ning Xiao, Jie Hu, Yi Yang, Zhiliang Sun, Yujuan Li
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引用次数: 0

摘要

替加环素是用于治疗耐多药细菌引起的严重感染的最后一种药物。tet(X4)是最近发现的一种质粒介导的替加环素耐药基因,可产生对替加环素和其他四环素的高级别耐药性。自2019年首次发现tet(X4)以来,它已在全球范围内迅速传播,因此,替加环素在临床治疗耐多药感染中的效果越来越差。在这项研究中,我们鉴定并分析了中国湖南省养鸭场分离出的 tet(X4)阳性大肠埃希菌。共从 9 个养鸭场采集了 976 个样本。通过抗菌药物敏感性测试和全基因组测序(WGS),确定了 tet(X4)阳性分离株的表型和基因型。此外,基于生物信息学分析和共轭,确定了 tet(X4) 的基因组特征和可转移性。因此,我们在鸭粪中检测到了一株携带 tet(X4) 和其他七个抗性基因的大肠杆菌。多焦点序列分型分析表明,该分离株属于一个新的克隆,随后的遗传分析表明,tet(X4)携带在一个4608-bp的环状中间体中,两侧有ISVsa3-ORF2-abh元件。此外,它还能以 10-5 的频率转移到大肠杆菌 C600 中。在养鸭场检测到携带 tet(X4)的大肠杆菌菌株加深了我们对替加环素耐药性动态的了解。tet(X4)循环中间体的可转移性导致了替加环素耐药基因的传播,这对医疗保健构成了巨大威胁。因此,有必要进行警惕性监测并采取积极措施防止其传播。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The ISVsa3-ORF2-abh-tet(X4) circular intermediate-mediated transmission of tigecycline resistance in Escherichia coli isolates from duck farms
Tigecycline is a last-resort drug used to treat serious infections caused by multidrug-resistant bacteria. tet(X4) is a recently discovered plasmid-mediated tigecycline resistance gene that confers high-level resistance to tigecycline and other tetracyclines. Since the first discovery of tet(X4) in 2019, it has spread rapidly worldwide, and as a consequence, tigecycline has become increasingly ineffective in the clinical treatment of multidrug-resistant infections. In this study, we identified and analyzed tet(X4)-positive Escherichia coli isolates from duck farms in Hunan Province, China. In total, 976 samples were collected from nine duck farms. Antimicrobial susceptibility testing and whole-genome sequencing (WGS) were performed to establish the phenotypes and genotypes of tet(X4)-positive isolates. In addition, the genomic characteristics and transferability of tet(X4) were determined based on bioinformatics analysis and conjugation. We accordingly detected an E. coli strain harboring tet(X4) and seven other resistance genes in duck feces. Multi-locus sequence typing analysis revealed that this isolate belonged to a new clone, and subsequent genetic analysis indicated that tet(X4) was carried in a 4608-bp circular intermediate, flanked by ISVsa3-ORF2-abh elements. Moreover, it exhibited transferability to E. coli C600 with a frequency of 10-5. The detection of tet(X4)-harboring E, coli strains on duck farms enhances our understanding of tigecycline resistance dynamics. The transferable nature of the circular intermediate of tet(X4) contributing to the spread of tigecycline resistance genes poses a substantial threat to healthcare. Consequently, vigilant monitoring and proactive measures are necessary to prevent their spread.
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来源期刊
CiteScore
7.90
自引率
7.00%
发文量
1817
审稿时长
14 weeks
期刊介绍: Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.
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