Chao Jiang, Jie Yang, Gang Xiao, Ning Xiao, Jie Hu, Yi Yang, Zhiliang Sun, Yujuan Li
{"title":"养鸭场分离出的大肠埃希菌中,ISVsa3-ORF2-abh-tet(X4)循环中间传播对替加环素的耐药性","authors":"Chao Jiang, Jie Yang, Gang Xiao, Ning Xiao, Jie Hu, Yi Yang, Zhiliang Sun, Yujuan Li","doi":"10.3389/fcimb.2024.1444031","DOIUrl":null,"url":null,"abstract":"Tigecycline is a last-resort drug used to treat serious infections caused by multidrug-resistant bacteria. <jats:italic>tet</jats:italic>(X4) is a recently discovered plasmid-mediated tigecycline resistance gene that confers high-level resistance to tigecycline and other tetracyclines. Since the first discovery of <jats:italic>tet</jats:italic>(X4) in 2019, it has spread rapidly worldwide, and as a consequence, tigecycline has become increasingly ineffective in the clinical treatment of multidrug-resistant infections. In this study, we identified and analyzed <jats:italic>tet</jats:italic>(X4)-positive <jats:italic>Escherichia coli</jats:italic> isolates from duck farms in Hunan Province, China. In total, 976 samples were collected from nine duck farms. Antimicrobial susceptibility testing and whole-genome sequencing (WGS) were performed to establish the phenotypes and genotypes of <jats:italic>tet</jats:italic>(X4)-positive isolates. In addition, the genomic characteristics and transferability of <jats:italic>tet</jats:italic>(X4) were determined based on bioinformatics analysis and conjugation. We accordingly detected an <jats:italic>E. coli</jats:italic> strain harboring <jats:italic>tet</jats:italic>(X4) and seven other resistance genes in duck feces. Multi-locus sequence typing analysis revealed that this isolate belonged to a new clone, and subsequent genetic analysis indicated that <jats:italic>tet</jats:italic>(X4) was carried in a 4608-bp circular intermediate, flanked by IS<jats:italic>Vsa3</jats:italic>-ORF2-<jats:italic>abh</jats:italic> elements. Moreover, it exhibited transferability to <jats:italic>E. coli</jats:italic> C600 with a frequency of 10<jats:sup>-5</jats:sup>. The detection of <jats:italic>tet</jats:italic>(X4)-harboring <jats:italic>E, coli</jats:italic> strains on duck farms enhances our understanding of tigecycline resistance dynamics. The transferable nature of the circular intermediate of <jats:italic>tet</jats:italic>(X4) contributing to the spread of tigecycline resistance genes poses a substantial threat to healthcare. Consequently, vigilant monitoring and proactive measures are necessary to prevent their spread.","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The ISVsa3-ORF2-abh-tet(X4) circular intermediate-mediated transmission of tigecycline resistance in Escherichia coli isolates from duck farms\",\"authors\":\"Chao Jiang, Jie Yang, Gang Xiao, Ning Xiao, Jie Hu, Yi Yang, Zhiliang Sun, Yujuan Li\",\"doi\":\"10.3389/fcimb.2024.1444031\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Tigecycline is a last-resort drug used to treat serious infections caused by multidrug-resistant bacteria. <jats:italic>tet</jats:italic>(X4) is a recently discovered plasmid-mediated tigecycline resistance gene that confers high-level resistance to tigecycline and other tetracyclines. Since the first discovery of <jats:italic>tet</jats:italic>(X4) in 2019, it has spread rapidly worldwide, and as a consequence, tigecycline has become increasingly ineffective in the clinical treatment of multidrug-resistant infections. In this study, we identified and analyzed <jats:italic>tet</jats:italic>(X4)-positive <jats:italic>Escherichia coli</jats:italic> isolates from duck farms in Hunan Province, China. In total, 976 samples were collected from nine duck farms. Antimicrobial susceptibility testing and whole-genome sequencing (WGS) were performed to establish the phenotypes and genotypes of <jats:italic>tet</jats:italic>(X4)-positive isolates. In addition, the genomic characteristics and transferability of <jats:italic>tet</jats:italic>(X4) were determined based on bioinformatics analysis and conjugation. We accordingly detected an <jats:italic>E. coli</jats:italic> strain harboring <jats:italic>tet</jats:italic>(X4) and seven other resistance genes in duck feces. Multi-locus sequence typing analysis revealed that this isolate belonged to a new clone, and subsequent genetic analysis indicated that <jats:italic>tet</jats:italic>(X4) was carried in a 4608-bp circular intermediate, flanked by IS<jats:italic>Vsa3</jats:italic>-ORF2-<jats:italic>abh</jats:italic> elements. Moreover, it exhibited transferability to <jats:italic>E. coli</jats:italic> C600 with a frequency of 10<jats:sup>-5</jats:sup>. The detection of <jats:italic>tet</jats:italic>(X4)-harboring <jats:italic>E, coli</jats:italic> strains on duck farms enhances our understanding of tigecycline resistance dynamics. The transferable nature of the circular intermediate of <jats:italic>tet</jats:italic>(X4) contributing to the spread of tigecycline resistance genes poses a substantial threat to healthcare. 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The ISVsa3-ORF2-abh-tet(X4) circular intermediate-mediated transmission of tigecycline resistance in Escherichia coli isolates from duck farms
Tigecycline is a last-resort drug used to treat serious infections caused by multidrug-resistant bacteria. tet(X4) is a recently discovered plasmid-mediated tigecycline resistance gene that confers high-level resistance to tigecycline and other tetracyclines. Since the first discovery of tet(X4) in 2019, it has spread rapidly worldwide, and as a consequence, tigecycline has become increasingly ineffective in the clinical treatment of multidrug-resistant infections. In this study, we identified and analyzed tet(X4)-positive Escherichia coli isolates from duck farms in Hunan Province, China. In total, 976 samples were collected from nine duck farms. Antimicrobial susceptibility testing and whole-genome sequencing (WGS) were performed to establish the phenotypes and genotypes of tet(X4)-positive isolates. In addition, the genomic characteristics and transferability of tet(X4) were determined based on bioinformatics analysis and conjugation. We accordingly detected an E. coli strain harboring tet(X4) and seven other resistance genes in duck feces. Multi-locus sequence typing analysis revealed that this isolate belonged to a new clone, and subsequent genetic analysis indicated that tet(X4) was carried in a 4608-bp circular intermediate, flanked by ISVsa3-ORF2-abh elements. Moreover, it exhibited transferability to E. coli C600 with a frequency of 10-5. The detection of tet(X4)-harboring E, coli strains on duck farms enhances our understanding of tigecycline resistance dynamics. The transferable nature of the circular intermediate of tet(X4) contributing to the spread of tigecycline resistance genes poses a substantial threat to healthcare. Consequently, vigilant monitoring and proactive measures are necessary to prevent their spread.
期刊介绍:
Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.