利用超高效液相色谱-Q-轨道rap-MS/MS分析法同时定性和定量测定茜草叶中的33种化合物

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-08-28 DOI:10.2174/0115734110314497240819053300

Xican Li, Jingyuan Zeng, Chunhou Li, Hanxiao Chai, Shaoman Chen, Nana Jin, Tingshan Chen, Xiaohua Lin, Sunbal Khan, Rongxin Cai

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引用次数: 0

摘要

背景介绍鲁冰花（Rubus alceifolius Poir）叶片是一种广泛分布于东半球的药用植物材料。然而，迄今为止还没有关于其化学成分的报告。研究方法采用一套实验方案处理新鲜的茜草叶，制备冻干水提取物。随后，采用数据库亲和型超高效液相色谱-四极杆-Exactive-Orbitrap-串联质谱（UHPLC-Q-Exactive-Orbitrap-MS/MS）技术对提取物进行定性和定量分析。结果：根据 UHPLC-Q-Exactive-Orbitrap-MS/MS 仪器的质谱/质谱阐释以及与数据库的比较，定性鉴定出 33 种化合物。特别是严格区分了 12 种异构体，包括芹菜素与 2'-hydroxydaidzein 异构体、叶黄素 7-O- 葡萄糖醛酸与黄芩苷异构体、(+) 儿茶素与 (-) 表儿茶素异构体、3'-羟基芹菜素与 2'-hydroxydaidzein 异构体。(-)表儿茶素、3-O-咖啡酰奎宁酸与 4-O-咖啡酰奎宁酸与 5-咖啡酰奎宁酸、3,4-O-二咖啡酰奎宁酸与 3,5-O-二咖啡酰奎宁酸与 4,5-二咖啡酰奎宁酸。此外，在负离子或正离子模型下还发现了 21 种非同分异构化合物，如鞣花酸和没食子酸。定量分析结果表明，鞣花酸的含量最高（133.00 ± 3.50 μg/g），而（+）-4-胆甾烯-3-酮的含量最低（0.035 ± 0.0050 μg/g）。结论这些发现将有助于了解白头翁叶传统药用功能的物质基础，并找到其合适的质量标记。

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Simultaneous Qualitative and Quantitative Determination of 33 Compounds from Rubus Alceifolius Poir Leaves Using UHPLC-Q-Orbitrap-MS/MS Analysis

Background: Rubus alceifolius Poir leaves are a widely distributed medicinal plant material in the Eastern Hemisphere. However, there has been no report regarding their chemistry until now. Methods: Fresh R. alceifolius leaves were treated using a set of experimental protocols to prepare a lyophilized aqueous extract. Subsequently, the extract was qualitative and quantitatively analyzed using the database-affinity ultra-high-performance liquid chromatography-quadrupole-Exactive- Orbitrap-tandem mass spectrometry (UHPLC-Q-Exactive-Orbitrap-MS/MS) technology. Results: Based on MS/MS elucidation and comparison with the database in UHPLC-Q-Exactive- Orbitrap-MS/MS apparatus, 33 compounds were qualitatively identified. Especially, 12 isomers were strictly distinguished, including apigenin vs. 2'-hydroxydaidzein, luteolin 7-O-glucuronide vs. scutellarin, (+) catechin vs. (-) epicatechin, 3-O-caffeoylquinic acid vs. 4-O-caffeoylquinic acid vs. 5-Ocaffeoylquinic acid, and 3,4-O-dicaffeoylquinic acid vs 3,5-O-dicaffeoylquinic acid vs 4,5-Odicaffeoylquinic acid. In addition, 21 non-isomeric compounds, such as ellagic acid and gallic acid, were also found under negative or positive ion models. The quantitative analysis suggested that ellagic acid was found to be of the highest level (133.00 ± 3.50 μg/g), while (+)-4-cholesten-3-one was calculated to be of the lowest level (0.035 ± 0.0050 μg/g). Conclusion: These findings will help to understand the substance basis of the traditional medicinal functions of R. alceifolius leaves and to find their suitable quality markers.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.