用于全动态时域心肌细胞膜再愈合评估的集成纳米多孔电穿孔和传感电极阵列

IF 8.1 1区 医学 Q1 ENGINEERING, BIOMEDICAL
Weiqin Sheng, Ying Li, Chunlian Qin, Zhonghai Zhang, Yuxiang Pan, Zhicheng Tong, Chong Teng, Xinwei Wei
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引用次数: 0

摘要

细胞内电生理研究对生物和医学研究至关重要。传统的平面微电极阵列(MEA)由于细胞-电极界面松散,在记录细胞内动作电位时存在缺陷。为了高灵敏度地研究细胞内电生理信号,人们采用电穿孔技术获得细胞内记录。本研究建立了一个基于纳米多孔电极阵列(NPEA)的生物传感系统,该系统集电穿孔和信号采集于一体,可长时间动态、灵敏地记录心肌细胞的胞内电位。此外,纳米多孔电极还能诱导细胞膜突起,增强细胞-电极界面耦合,从而促进有效电穿孔。整个记录过程中的电生理信号可根据信号变化进行定量和分段分析,从而等效反映电穿孔心肌细胞膜的动态演变。我们相信,本研究提出的低成本、高性能的纳米多孔生物传感平台可以动态记录细胞内动作电位,评估心肌细胞电穿孔,为心内科药理学研究提供一种新的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Integrated nanoporous electroporation and sensing electrode array for total dynamic time-domain cardiomyocyte membrane resealing assessment

Integrated nanoporous electroporation and sensing electrode array for total dynamic time-domain cardiomyocyte membrane resealing assessment

Intracellular electrophysiological research is vital for biological and medical research. Traditional planar microelectrode arrays (MEAs) have disadvantages in recording intracellular action potentials due to the loose cell–electrode interface. To investigate intracellular electrophysiological signals with high sensitivity, electroporation was used to obtain intracellular recordings. In this study, a biosensing system based on a nanoporous electrode array (NPEA) integrating electrical perforation and signal acquisition was established to dynamically and sensitively record the intracellular potential of cardiomyocytes over a long period of time. Moreover, nanoporous electrodes can induce the protrusion of cell membranes and enhance cell–electrode interfacial coupling, thereby facilitating effective electroporation. Electrophysiological signals over the entire recording process can be quantitatively and segmentally analyzed according to the signal changes, which can equivalently reflect the dynamic evolution of the electroporated cardiomyocyte membrane. We believe that the low-cost and high-performance nanoporous biosensing platform suggested in this study can dynamically record intracellular action potential, evaluate cardiomyocyte electroporation, and provide a new strategy for investigating cardiology pharmacological science.

Graphic abstract

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来源期刊
Bio-Design and Manufacturing
Bio-Design and Manufacturing Materials Science-Materials Science (miscellaneous)
CiteScore
13.30
自引率
7.60%
发文量
148
期刊介绍: Bio-Design and Manufacturing reports new research, new technology and new applications in the field of biomanufacturing, especially 3D bioprinting. Topics of Bio-Design and Manufacturing cover tissue engineering, regenerative medicine, mechanical devices from the perspectives of materials, biology, medicine and mechanical engineering, with a focus on manufacturing science and technology to fulfil the requirement of bio-design.
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