Késsia Fátima Cunha Pantoja, Alessandra de Jesus Boari, Bruno Rossitto De Marchi, Jorge Alberto Marques Rezende, Elliot Watanabe Kitajima, Rivadalve Coelho Gonçalves, Giselle Mariano Lessa Assis, Renate Krause-Sakate
{"title":"巴西牧草花生（Arachis pintoi）中豇豆萎黄斑驳病毒的检测和特征描述","authors":"Késsia Fátima Cunha Pantoja, Alessandra de Jesus Boari, Bruno Rossitto De Marchi, Jorge Alberto Marques Rezende, Elliot Watanabe Kitajima, Rivadalve Coelho Gonçalves, Giselle Mariano Lessa Assis, Renate Krause-Sakate","doi":"10.1007/s13313-024-00987-3","DOIUrl":null,"url":null,"abstract":"<div><p>The cowpea chlorotic mottle virus (CCMV) was detected in forage peanut plants (<i>Arachis pintoi</i>), showing mosaic, yellowing, and interveinal chlorosis. Negatively stained partially purified preparations contained isometric particles ca. 30 nm in size, also present in the cytoplasm and vacuole of leaf parenchymal cells from CCMV-infected <i>A. pintoi</i> leaves. Mechanical transmission of the CCMV leaf extracts from symptomatic <i>A. pintoi</i> plants resulted in systemic mosaic in <i>A. pintoi</i> cv. BRS Mandobi, <i>Vigna unguiculata</i> L. cv Pretinha, and <i>V. unguiculata</i> sg. <i>sesquipedalis</i> cv. De Metro plants; mottle on leaves of inoculated <i>Nicotiana occidentalis</i> and <i>N. benthamiana</i>; and local necrotic and chlorotic lesions in <i>Chenopodium quinoa</i> and <i>C. amaranticolor,</i> respectively. High-throughput sequencing (HTS) allowed us to identify the causal agent as the CCMV and obtain the complete nucleotide sequence of the three genome components. BLAST search in the nucleotide database revealed high identities of the genome components with previously reported CCMV isolates. Primer was designed based on the HTS-derived sequence and efficiently detected CCMV in the infected <i>A. pintoi</i> samples. To our knowledge, this is the first report of CCMV infecting forage peanuts.</p></div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"53 5","pages":"385 - 389"},"PeriodicalIF":0.9000,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection and characterization of the cowpea chlorotic mottle virus in forage peanut (Arachis pintoi) in Brazil\",\"authors\":\"Késsia Fátima Cunha Pantoja, Alessandra de Jesus Boari, Bruno Rossitto De Marchi, Jorge Alberto Marques Rezende, Elliot Watanabe Kitajima, Rivadalve Coelho Gonçalves, Giselle Mariano Lessa Assis, Renate Krause-Sakate\",\"doi\":\"10.1007/s13313-024-00987-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The cowpea chlorotic mottle virus (CCMV) was detected in forage peanut plants (<i>Arachis pintoi</i>), showing mosaic, yellowing, and interveinal chlorosis. Negatively stained partially purified preparations contained isometric particles ca. 30 nm in size, also present in the cytoplasm and vacuole of leaf parenchymal cells from CCMV-infected <i>A. pintoi</i> leaves. Mechanical transmission of the CCMV leaf extracts from symptomatic <i>A. pintoi</i> plants resulted in systemic mosaic in <i>A. pintoi</i> cv. BRS Mandobi, <i>Vigna unguiculata</i> L. cv Pretinha, and <i>V. unguiculata</i> sg. <i>sesquipedalis</i> cv. De Metro plants; mottle on leaves of inoculated <i>Nicotiana occidentalis</i> and <i>N. benthamiana</i>; and local necrotic and chlorotic lesions in <i>Chenopodium quinoa</i> and <i>C. amaranticolor,</i> respectively. High-throughput sequencing (HTS) allowed us to identify the causal agent as the CCMV and obtain the complete nucleotide sequence of the three genome components. BLAST search in the nucleotide database revealed high identities of the genome components with previously reported CCMV isolates. Primer was designed based on the HTS-derived sequence and efficiently detected CCMV in the infected <i>A. pintoi</i> samples. To our knowledge, this is the first report of CCMV infecting forage peanuts.</p></div>\",\"PeriodicalId\":8598,\"journal\":{\"name\":\"Australasian Plant Pathology\",\"volume\":\"53 5\",\"pages\":\"385 - 389\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2024-09-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Australasian Plant Pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13313-024-00987-3\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Australasian Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s13313-024-00987-3","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Detection and characterization of the cowpea chlorotic mottle virus in forage peanut (Arachis pintoi) in Brazil
The cowpea chlorotic mottle virus (CCMV) was detected in forage peanut plants (Arachis pintoi), showing mosaic, yellowing, and interveinal chlorosis. Negatively stained partially purified preparations contained isometric particles ca. 30 nm in size, also present in the cytoplasm and vacuole of leaf parenchymal cells from CCMV-infected A. pintoi leaves. Mechanical transmission of the CCMV leaf extracts from symptomatic A. pintoi plants resulted in systemic mosaic in A. pintoi cv. BRS Mandobi, Vigna unguiculata L. cv Pretinha, and V. unguiculata sg. sesquipedalis cv. De Metro plants; mottle on leaves of inoculated Nicotiana occidentalis and N. benthamiana; and local necrotic and chlorotic lesions in Chenopodium quinoa and C. amaranticolor, respectively. High-throughput sequencing (HTS) allowed us to identify the causal agent as the CCMV and obtain the complete nucleotide sequence of the three genome components. BLAST search in the nucleotide database revealed high identities of the genome components with previously reported CCMV isolates. Primer was designed based on the HTS-derived sequence and efficiently detected CCMV in the infected A. pintoi samples. To our knowledge, this is the first report of CCMV infecting forage peanuts.
期刊介绍：
Australasian Plant Pathology presents new and significant research in all facets of the field of plant pathology. Dedicated to a worldwide readership, the journal focuses on research in the Australasian region, including Australia, New Zealand and Papua New Guinea, as well as the Indian, Pacific regions.
Australasian Plant Pathology is the official journal of the Australasian Plant Pathology Society.
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