G. da Silva Dias, J. T. L. Chaves, T. R. S. Santos, Q. S. Garcia, M. A. S. Artur, E. M. Bicalho
{"title":"做好准备:Tabebuia heterophylla 种子中的非连续水合作用如何诱导幼苗的抗逆性?","authors":"G. da Silva Dias, J. T. L. Chaves, T. R. S. Santos, Q. S. Garcia, M. A. S. Artur, E. M. Bicalho","doi":"10.1111/plb.13719","DOIUrl":null,"url":null,"abstract":"<jats:list list-type=\"bullet\"> <jats:list-item>Discontinuous hydration and dehydration (HD) cycles refer to controlled imbibition followed by dehydration before seed germination. Here, we investigated whether the level of imbibition before HD cycles affects the physiology of <jats:italic>Tabebuia heterophylla</jats:italic> seeds and seedlings.</jats:list-item> <jats:list-item>Seeds were imbibed for 10 h (T1; phase I of imbibition) or 35 h (T2; phase II), dehydrated, and progressively rehydrated one to four times (HD cycles). Germination and biochemical parameters (membrane integrity; total soluble, reducing, and nonreducing (NRS) sugars; proteins, amino acids, proline, H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>, catalase, ascorbate peroxidase, and glutathione reductase activity) were quantified at the last rehydration step of each cycle. Biometric and biochemical parameters (including pigments) were analysed in seedlings 60 days after germination.</jats:list-item> <jats:list-item>HD cycles at T1 led to reduced seed germination and greater plasma membrane damage, higher enzyme activity (catalase and glutathione reductase) and accumulation of NRS, total amino acids, and proline compared to the controls and T2 treatment. Cellular damage became more severe with more HD cycles. HD cycles at T2 synchronized germination regardless of the number of cycles and also had a priming effect. T2 seeds had less NRS, total amino acids, and proline content than T1. HD cycles at T1 produced seedlings with higher carotenoid and total chlorophyll content than controls and T2, while seedlings from HD cycles at T2 had higher amounts of osmoprotectants.</jats:list-item> <jats:list-item>HD cycles at T2 benefited seeds and seedlings more than at T1. This suggests that the physiological and biochemical effects of HD cycles in seeds modulate seedling plasticity, depending on water availability, potentially promoting increased tolerance to recurrent droughts that will be intensified with ongoing climate changes.</jats:list-item> </jats:list>","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":null,"pages":null},"PeriodicalIF":4.2000,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Be prepared: how does discontinuous hydration in Tabebuia heterophylla seeds induce stress tolerance in seedlings?\",\"authors\":\"G. da Silva Dias, J. T. L. Chaves, T. R. S. Santos, Q. S. Garcia, M. A. S. Artur, E. M. Bicalho\",\"doi\":\"10.1111/plb.13719\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<jats:list list-type=\\\"bullet\\\"> <jats:list-item>Discontinuous hydration and dehydration (HD) cycles refer to controlled imbibition followed by dehydration before seed germination. Here, we investigated whether the level of imbibition before HD cycles affects the physiology of <jats:italic>Tabebuia heterophylla</jats:italic> seeds and seedlings.</jats:list-item> <jats:list-item>Seeds were imbibed for 10 h (T1; phase I of imbibition) or 35 h (T2; phase II), dehydrated, and progressively rehydrated one to four times (HD cycles). Germination and biochemical parameters (membrane integrity; total soluble, reducing, and nonreducing (NRS) sugars; proteins, amino acids, proline, H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>, catalase, ascorbate peroxidase, and glutathione reductase activity) were quantified at the last rehydration step of each cycle. 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Be prepared: how does discontinuous hydration in Tabebuia heterophylla seeds induce stress tolerance in seedlings?
Discontinuous hydration and dehydration (HD) cycles refer to controlled imbibition followed by dehydration before seed germination. Here, we investigated whether the level of imbibition before HD cycles affects the physiology of Tabebuia heterophylla seeds and seedlings.Seeds were imbibed for 10 h (T1; phase I of imbibition) or 35 h (T2; phase II), dehydrated, and progressively rehydrated one to four times (HD cycles). Germination and biochemical parameters (membrane integrity; total soluble, reducing, and nonreducing (NRS) sugars; proteins, amino acids, proline, H2O2, catalase, ascorbate peroxidase, and glutathione reductase activity) were quantified at the last rehydration step of each cycle. Biometric and biochemical parameters (including pigments) were analysed in seedlings 60 days after germination.HD cycles at T1 led to reduced seed germination and greater plasma membrane damage, higher enzyme activity (catalase and glutathione reductase) and accumulation of NRS, total amino acids, and proline compared to the controls and T2 treatment. Cellular damage became more severe with more HD cycles. HD cycles at T2 synchronized germination regardless of the number of cycles and also had a priming effect. T2 seeds had less NRS, total amino acids, and proline content than T1. HD cycles at T1 produced seedlings with higher carotenoid and total chlorophyll content than controls and T2, while seedlings from HD cycles at T2 had higher amounts of osmoprotectants.HD cycles at T2 benefited seeds and seedlings more than at T1. This suggests that the physiological and biochemical effects of HD cycles in seeds modulate seedling plasticity, depending on water availability, potentially promoting increased tolerance to recurrent droughts that will be intensified with ongoing climate changes.
期刊介绍:
Plant Biology is an international journal of broad scope bringing together the different subdisciplines, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, and mycology.
Plant Biology publishes original problem-oriented full-length research papers, short research papers, and review articles. Discussion of hot topics and provocative opinion articles are published under the heading Acute Views. From a multidisciplinary perspective, Plant Biology will provide a platform for publication, information and debate, encompassing all areas which fall within the scope of plant science.