1 型糖尿病患者外周血单核细胞的代谢紊乱

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-12 DOI:10.1007/s10517-024-06201-0

V. V. Ivanov, E. E. Buyko, A. A. Ufandeev, K. V. Nevskaya, Ya. S. Slavkina, E. V. Udut, T. V. Saprina, V. V. Udut

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引用次数: 0

摘要

流式细胞荧光测定法评估了 1 型糖尿病患者和健康志愿者外周血单核细胞中 ROS（荧光探针 2',7'-二氯二氢荧光素二乙酸酯）和脂质含量（荧光亲脂染料尼罗河红）的水平。使用特异性抗体评估 CD36+ 单核细胞的数量。在 1 型糖尿病患者中，单核细胞中的 ROS 和细胞内脂质水平以及表达 CD36 脂肪酸转运酶的细胞数量均升高。这些结果表明，碳水化合物代谢紊乱患者的外周血细胞发生了新陈代谢变化，可被视为1型糖尿病并发症发生的可能预后标志。

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Dysmetabolism of Peripheral Blood Monocytes in Type 1 Diabetes Mellitus

The level of ROS (fluorescent probe 2’,7’-dichlorodihydrofluorescein diacetate) and lipid content (fluorescent lipophilic dye Nile Red) in the peripheral blood monocyte fraction from patients with type 1 diabetes mellitus and healthy volunteers were assessed by flow cytofluorimetry. The number of CD36⁺ monocytes was assessed using specific antibodies. In patients with type 1 diabetes mellitus, the levels of ROS and intracellular lipids in monocytes and the number of cells expressing CD36 fatty acid translocase were elevated. These results indicate metabolic changes in the peripheral blood cells of patients with carbohydrate metabolism disorders and can be considered as possible prognostic markers for the development of type 1 diabetes mellitus complications.

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.