表达神经生长因子的转导嗅粘膜细胞用于治疗实验性脊髓囊肿

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-12 DOI:10.1007/s10517-024-06222-9

O. V. Stepanova, G. A. Fursa, S. S. Andretsova, E. K. Karsuntseva, V. S. Shishkina, A. V. Chadin, A. D. Voronova, A. S. Semkina, I. V. Reshetov, V. P. Chekhonin

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引用次数: 0

摘要

我们开发了一种表达神经生长因子（NGF）的新型基因细胞构建体。在获得工程化腺病毒载体Ad5-RGD-CAG-NGF和Ad5-RGD-CAG-EGFP后，研究了转导效率和转基因表达，并确定了感染倍数。转导的表达 NGF 的人嗅鞘细胞在有限的时间内恢复了大鼠的运动能力。基因-细胞构建体移植后大鼠后肢活动能力的改善和囊肿体积的缩小更有可能是由于构建体的细胞成分所致。

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Transduced Olfactory Mucosa Cells Expressing Nerve Growth Factor for the Therapy of Experimental Spinal Cord Cysts

A new gene-cell construct expressing nerve growth factor (NGF) has been developed. After obtaining engineered adenovectors Ad5-RGD-CAG-NGF and Ad5-RGD-CAG-EGFP, transduction efficiency and transgene expression were studied and multiplicity of infection was determined. The efficacy of transduced human olfactory ensheathing cells expressing NGF in restoring motor activity in rats has been shown in a limited period of time. Improved rat hindlimb mobility and cyst size reduction after gene-cell construct transplantation were more likely due to the cellular component of the construct.

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.