异源表达硫：醌氧化还原酶（Sqr）以提高硫代卤化弧菌 D301 的脱硫性能。

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2024-09-11 DOI:10.1093/jambio/lxae237

Zhuang Tian,Chenxi Yu,He Li,Bin Song,Jianwei Lv,Chuang Liu,Wanting Sun,Xiao Hu,Yixi Xiong,Jinlong Liu,Xiyu Cheng,Maohua Yang,Jianmin Xing

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引用次数: 0

摘要

AIMSHeterologous expression of sulfur:quinone oxidoreductase (Sqr) from Halomonas mongoliensis JS01, which is responsible to oxidizing sulfide to elemental sulfur, in Thioalkalivibrio versutus (T. versutus)D301 improves desulfurization.Method and RESULTS We expressed sqr in T. versutus D301 by conjugative transfer.方法与结果我们通过共轭转移法在全氟弧菌 D301 中表达了 sqr，然后在空运反应器中测定了其脱硫能力，并分析了其在 -380 mV ORP 条件下的转录组。我们的研究结果表明，D301-sqr+ 菌株在最佳 ORP 条件（-380mV）下利用硫化钠作为硫源，元素硫产量达到 95%。这比 T. versutus D301 提高了 8%。此外，D301-sqr+的硫化钠利用率也有显著提高（0.741 vs. 0.651 mmol∙(L-h)-1），与T. versutus D301（0.716 vs. 0.518 mmol∙(L-h)-1）相比，元素硫的产生率也同时提高。结论异源表达 sqr 基因改变了 T. versutus D301 硫氧化途径中相关基因的转录，提高了元素硫的产量和硫氧化速率，使 D301-sqr+ 更具工业应用潜力。

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Heterologous expression of sulfur:quinone oxidoreductase (Sqr) to improve Thioalkalivibrio versutus D301 desulfurization performance.

AIMS Heterologous expression of sulfur:quinone oxidoreductase (Sqr) from Halomonas mongoliensis JS01, which is responsible for oxidizing sulfide to elemental sulfur, in Thioalkalivibrio versutus (T. versutus)D301 improves desulfurization. METHODS AND RESULTS We expressed sqr in T. versutus D301 by conjugative transfer and then assayed its desulfurization capacity in an airlift reactor and analyzed its transcriptome at -380 mV ORP. Our findings demonstrate that the D301-sqr+ strain, utilizing sodium sulfide as a sulfur source under optimal ORP conditions (-380mV), achieved an elemental sulfur yield of 95%. This represents an 8% increase over the T. versutus D301. Moreover, the sodium sulfide utilization rate for D301-sqr+ showed a marked improvement (0.741 vs. 0.651 mmol∙(L·h)-1), with a concurrent increase in the rate of elemental sulfur production when compared to the T. versutus D301 (0.716 vs. 0.518 mmol∙(L·h)-1). Transcriptome analysis revealed that the flavocytochrome c (fcc) and the sox system were differentially transcriptionally down-regulated in D301-sqr+ compared with the T. versutus D301. CONCLUSIONS Heterologous expression of the gene sqr altered the transcription of related genes in T. versutus D301 sulfur oxidation pathway, increasing the yield of elemental sulfur and the rate of sulfur oxidation, and making D301-sqr+ more potential for industrial applications.

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.