结合肠道微生物群和转录组分析研究不同放养密度对太平洋南美白对虾(Litopenaeus vannamei)利用小球藻(Chlorella sorokiniana)能力的影响

IF 6.1 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Hang Yuan, Minghua Xie, Jian Chen, Naijie Hu, Honming Wang, Beiping Tan, Lili Shi, Shuang Zhang
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引用次数: 0

摘要

为了研究不同放养密度对太平洋南美白对虾()利用(CHL)能力的影响,本研究采用了 3 × 2 因式设计放养实验。具体而言,在低密度(LSD;每米 100 只)、中密度(MSD;每米 200 只)和高密度(HSD;每米 300 只)放养密度下,用两种食物蛋白源(鱼粉 [FM] 和 CHL)喂养对虾 8 周。随着放养密度的增加,对虾的生长性能和抵抗力(1.0 × 10 CFU/mL)下降,但日粮 CHL 改善了这一结果。只有在高密度条件下,CHL 组和 FM 组的抗逆性差异才显著(< 0.05)。饲养密度和蛋白质来源对过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和酚氧化酶(PO)的活性,以及肝胰腺中丙二醛(MDA)的含量和肠道淀粉酶的活性都有显著的交互作用,其中大部分只有在高饲养密度下 CHL 组和 FM 组之间才有显著差异 ( < 0.05)。16S rDNA 测序分析表明,日粮 CHL 增加了肠道微生物群的α多样性,抑制了病原菌的定植,提高了有益菌的丰度。转录组学结果显示,在高饲养密度下,FM 组与 CHL 组的差异表达基因(DEGs)大多上调,且主要富集在免疫和代谢相关通路中,包括 Toll、免疫缺陷(Imd)和糖酵解-葡萄糖生成通路。皮尔逊相关分析表明,前十种肠道细菌属水平与明显富集的 DEGs 之间存在显著相关性,而且在高密度情况下检测到的 DEGs 更多。总之,CHL 作为一种新型蛋白质来源在对虾集约化养殖中具有巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Combined intestinal microbiota and transcriptomic analysis to investigate the effect of different stocking densities on the ability of Pacific white shrimp (Litopenaeus vannamei) to utilize Chlorella sorokiniana
Aiming to investigate the impact of different stocking densities on the ability of Pacific white shrimp () to utilize (CHL), a 3 × 2 factorial design stocking experiment was used in this study. Specifically, shrimp was fed with two dietary protein sources (fishmeal [FM] and CHL) at low (LSD; 100 per m), medium (MSD; 200 per m) and high (HSD; 300 per m) stocking densities for 8 weeks. The growth performance and resistance to (1.0 × 10 CFU/mL) of shrimp decreased with the increase of stocking density, but dietary CHL improved this result. Differences between the CHL and FM groups for . resistance were significant only under high-density conditions ( < 0.05). Significant interactions between stocking density and protein source were found on the activities of catalase (CAT), superoxide dismutase (SOD) and phenol oxidase (PO), and the contents of malondialdehyde (MDA) in the hepatopancreas and the activities of intestinal amylase, most of which were significantly different between CHL and FM groups only at high stocking density ( < 0.05). Analysis of 16S rDNA sequencing showed that dietary CHL increased the alpha diversity of intestinal microbiota, inhibited the colonization of pathogenic bacteria and enhanced the abundance of beneficial bacteria. Transcriptomic results showed that at high stocking densities, differentially expressed genes (DEGs) in the FM vs CHL group were mostly upregulated and primarily enriched in immune and metabolic related pathways including Toll, immune deficiency (Imd) and glycolysis–gluconeogenesis pathways. Pearson correlation analysis revealed significant correlation between the top ten intestinal bacteria at the genus level and markedly enriched DEGs, also more were detected under high density situations. In conclusion, CHL has great potential as a novel protein source in the intensive farming of shrimp.
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来源期刊
Animal Nutrition
Animal Nutrition Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
7.40
自引率
3.20%
发文量
172
审稿时长
12 weeks
期刊介绍: Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to nutrition, and more applied aspects of animal nutrition, such as raw material evaluation, feed additives, nutritive value of novel ingredients and feed safety.
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